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Interspecies comparison of plasma protein binding of two radiolabelled complexes
Smolová, Lenka ; Trejtnar, František (referee) ; Lázníček, Milan (advisor)
This paper engages in the newly prepared bifunctional chelate DTPA-oxn which has a potential use in radiolabelling biological macromolecules. The aim of the paper was to prepare a radiolabelled bifunctional chelate DTPA-oxn with a suitable radionuclide, the 90Y has been used. Binding of 90Y-DTPA-oxn to human, bovine, rabbit and rat plasma protein was determined. An equilibrium dialysis at 37řC was employed for the plasma protein binding determination. The received results received were compared with the results of plasma protein binding of a clinically routinely used bifunctional chelate DTPA radiolabelled with 99mTc (99mTc-DTPA). The results showed a low plasma protein binding of these radiolabelled chelates. Interspeices comparison demonstrated that the results obtained for human, bovine and rat plasma are comparable, whereas those obtained for rabbit plasma are higher. This brings a conclusion that the binding of 90Y-DTPA- oxn and 99mTc-DTPA to plasma protein is not a factor that can influence their biological behavior.
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Preparation of radiolabeled albumin
Suchan, Martin ; Trejtnar, František (advisor) ; Lázníček, Milan (referee)
Objectives: This senior thesis was aimed at determining a method of albumin labelling with a particular radionuclide - technetium 99m Tc. Our task was to review the various possible methods needed for working out a procedure for albumin labelling with the mentioned radioisotope and also to test the behavior of labelled albumin in vitro in experiments with newly-isolated rat kidney cells. The next objectives of this work were to analyze the performed experiments, compare the results with other known procedures of albumin labelling with technetium 99m Tc and cast the results in the form of a scientific statement. Methods: A low pH and high concentration of albumin were useful for the albumin labelling experiments. A solution of SnCl2.2H2O was used for the reduction of pertechnetate. Purification of labelled albumin was performed by gel chromatography with a Sephadex G-50. The locations of albumin peaks in the chromatographic fractions were determined by the bicinchonic method. The location of pertechnetate was found by investigating the chromatographic spectrum of pertechnetate. Investigation of the elution spectrum was used to check for proper labelling. The radiochemical purity of labelled albumin was verified by paper chromatography and subsequent detection. Newly- labelled albumin was used in the...
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Preparation of 64Cu and its prospective carriers for nuclear medicine.
Kozempel, Ján ; Lešetický, Ladislav (advisor) ; Kotek, Jan (referee) ; John, Jan (referee) ; Příhoda, Jiří (referee)
5 Summary The preparation of 64Cu including it's radiochemical separation and the preparation of its novel radiolabelled tracers intended for the use in nuclear medicine is described in this work. A novel method for the preparation of no carrier added 64Cu, based on the 64Zn(d,2p)64Cu reaction and consecutive dual ion exchange chromatography was developed. The radiochemical product was of high specific activity and with only 61Cu radionuiclidic impurity, which allows it's use for in vitro laboratory experiments or in vivo preclinical tests of labelled tracers. We have focused on the preparation of several 64Cu labelled tracers. Two different types - water soluble polymer carriers and hypoxia selective biacetyl bis- (N4-alkylthiosemicarbazone) complexes of 64Cu were prepared. The water soluble polymer carriers based on the copolymers of NIPAM, GM and HPMA bearing various chelating groups were prepared. The polymeric chain was modified by copolymerization of a monomer with chelating functional group or modified after polymerization. Two standard polyacidic chelators (DOTA, DTPA) as well as three non-common copper chelators (dipicolylamine, thiosemicarbazide, Ag ionophore II) were used. Their potential use in nuclear medicine for local radio- or combined ...
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