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Viscometric study of L-Lysine- and 6-aminocaproic acid-hyaluronan systems behavior within the range of low aminoacids concentrations
Šimáčková, Marcela ; Běťák, Jiří (referee) ; Chytil, Martin (advisor)
This bachelor thesis investigates the interaction between high-molecular weight hyaluronic acid (1.75 MDa HA) and amino acids, particularly lysine and 6-aminocaproic acid. The interactions should occur between the carboxyl groups of HA carrying a negative charge and a positive charge located on the nitrogen atom of the protonated amino acids. The presence of these interactions would mean the possibility of establishing bioactive compounds, e.g. drugs, on hyaluronic acid, in which it would act as a targetting carrier. The interactions were examined at low concentrations for both aminoacids, i.e. below 10 mmoldm-3, and for 6-aminocaproic acid also at high aminoacid concentrations, i.e. above 20 mmoldm-3. The presence of interaction was investigated using not only rheology, but also by measuring pH and conductivity. Based on this research, the interactions manifested in terms of a decrease in the system viscosity.
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Need for self-actualization in patients during hospitalization
VACHTOVÁ, Ivana
Human personality is a dynamic and organized set of characteristics which are in a special relation. Personatity is thought to be determined largely by either genetics and heredity, or by environment and experience, or both. The personal needs are dependent on sex, age, education and differ in different generations. The personality is developed through interaction of life situation, life conditions and the place. Self-fulfillment means to fulfill one´s potencial. It refers to ability to realize one´s own needs and plans. According to Maslow the need of self-actualization belongs to growth needs. Self-realization evokes feelings of happiness and joy. It surpasses everyday routine, it is a spiritual need. The first objective of my thesis was to examine the need of self-realization in hospitalized patients. The second objective was to compare the needs of patients hospitalized in intensive care units to those of patients admitted to planned medical interventions and to those of patients in long-term care facilities. The third objective was to find out nurses´ attitude to hospitalized patients with needs of self-realization. To find relevant data patients hospitalized in intensive care units, patients in long-term care facilities and patients with planned medical interventions were addressed. Nurses taking care of those patients were also asked to take part in the research . Data were collected by the questionnaire method. The hypothesis I suggests that patients treated in intensive care units prefer the treatment which leads to feelings of security and alleviates pain. This hypothesis was confirmed. The hypothesis II suggests that patients admitted to planned medical intervention have more intensive needs of self-realization than patients treated in intensive care units. This hypothesis was not confirmed {--} patients expecting interventions feel the same need of security and pain management as patients hospitalized in intensive care units. The hypotesis III that patients admitted to planned interventions have more intensive needs of self-realization than patients staying in long-term care facilities was not confirmed. According to the data collected, patients in long-term care facilities need pain management and feelings of security, but they also need to be respected as personalities. They prefer to have the strength to fight the disease to the need of having the bed properly made. The hypothesis IV that nurses do not show enough interest in hospitalized patients´needs of self-realization was not confirmed. Nurses are informed enough about patients´ needs. They usually try hard to find the time to talk to patients. The results should make nurses be more aware of the need of lifelong learning despite their enormous workload and care for their own mental and physical health by following principles of mental hygiene. Key words: needs self- fulfillment patient environment relations communication
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Aggregation processes in amphiphil-polymer system
Šejnohová, Michaela ; Márová, Ivana (referee) ; Pekař, Miloslav (advisor)
This Bachelor´s thesis is focused on the exploration of polyelectrolyte-surfactant system by fluorescence spectroscopy with pyrene as fluorescent probe. Cationic cetyltrimethylammonium bromide (CTAB) was used as a surfactant. Native hyaluronan was used as polyelectrolyte. Hyaluronan was used in one constant molecular weight and one constant concentration. The whole system was prepared in an aqueous environment. Interactions were studied in area between critical aggregation concentration (CAC) and critical micelle concentration (CMC). The influences of the concentration of pyrene and the effect of the way of the samples preparation on aggregation behavior were also studied. It was found that above the critical aggregation concentration exists an area where stable properties of aggregates can be found. The concentration of pyrene has a major effect on the fluorescence properties of the system. Preparation of samples affects the aggregation of the system. At higher surfactant concentrations, above the stable area, was observed phase separation and gel formation.
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Characterization and stabilization of pancreatin
Wurstová, Agáta ; Němcová, Andrea (referee) ; Obruča, Stanislav (advisor)
This work focuses on a study of enzyme mixture pancreatin, its characterization and subsequent encapsulation into liposomes. As a reference proteins bovine serum albumin and trypsin were used. Characterization of pancreatin consisted of two parts. The first part focuses on optimization of methods for the concentration determination by absorption spectrophotometry using basic methods for identifying proteins (Biuret method, Hartree-Lowry method and Bradford method). Moreover, UV spectrums of the protein were measured. As a method for identification of protein´s molecular weight, SDS-PAGE was used. To identify components of pancreatin, LPLC was employed in two modifications, ion-exchange chromatography and size exclusion chromatography. The second part is dedicated to the characterization of pancreatin as enzyme in terms of pH and temperature optimum for the enzyme activities of protease (pH 9, 8 and 50 °C), amylase (pH 7 and 40 °C) and lipase (pH 7 and 50 °C). The last part of this work aimed at an encapsulation of pancreatin into liposomes and DLS analysis of distribution of particles and their zeta potential. Liposomes did not spontaneously release encapsulated enzyme. To confirm that proteins were successfully entrapped into liposomes, their structure was disrupted by application of phospholipase D. In conclusion, liposomes can be utilized as delivery systems for native enzymes.
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Influence of pH on polyectrolyte-surfactant system
Mourycová, Jana ; Omelka, Ladislav (referee) ; Pekař, Miloslav (advisor)
In this bachelor thesis, was studied influence of pH on aggregation polyelectrolyte-surfactant system using fluorescence spectroscopy and pyrene as fluorescent probe was used. As polyelectrolyte, hyaluronan with different molecular weight was used. Two surfactants cationic surfactant cetyltrimethylamonium bromide (CTAB) and nonionic surfactants polyoxyethylene (20) (TWEEN 20) were selected for interaction with hyaluronan. In the pH range from 3 to 10, no signifact changes in the aggregate non-polar core was observed. This mean system is stable against changes in the observed pH range. At system with higher concentrations of cationic surfactant (CTAB) and higher molecular weight hyaluronan occurred phase separation and gel exclusion was observed.
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Hyaluronan ionocomplexes for cosmetic and farmaceutical applications
Cimalová, Jana ; Obruča, Stanislav (referee) ; Pekař, Miloslav (advisor)
This bachelor thesis is focused on the study of a system of cationic surfactant – hyaluronan. Using fluorescent spectroscopy the critical micellar concentration of Carbethopendecinium bromide (Septonex) was determined in three environments – water, 0,15 M NaCl, and buffer PBS, with three fluorescent probes – pyrene, perylene, and nile red. It was found that the physiological environments (buffer and NaCl) reduce the value of CMC about one order. During the experiments with native hyaluronan, precipitate and gel formation were observed. The following part dealt with aggregate behaviour of the new biologically acceptable amphoteric surfactant tetradecylfosfocholine (TPC) in the environment of water and NaCl according to the increasing molar molecular weight of hyaluronan. As a fluorescent probe perylene was used. These measurements showed that the molar molecular weight or a change of ionic force has no effect on the value of CMC and therefore there are no interactions with hyaluronan. The last surfactant to study was 1,2-dihexadecanoyl-3-dimetylammonium-propan (DPTAP), which is water insoluble. DPTAP is soluble in chloroform, but after evaporation of chloroform and after addition of hyaluronan, it precipitated. The results so far, have confirmed that in terms of research of aggregation by fluorescence spectroscopy the new cationic surfactants TPC and DPTAP are not suitable for complexation with hyaluronan.
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Characterization of plasma during organosilicone thin film deposition using haxamethyldisiloxane monomer
Blahová, Lucie ; Mazánková, Věra (referee) ; Krčma, František (advisor)
The aim of this thesis is plasma diagnostic during deposition of thin films based on organosilicones. Hexamethyldisiloxane was used as a precursor for plasma deposition in the presence of oxygen, and the diagnosis was performed by using optical emission spectroscopy. The theoretical part summarizes the basic characteristics of plasma and processes occurred at plasma polymerization and deposition. It also deals with thin films and their use in coating materials. A relatively large part is devoted to the specification of the physical-chemical nature of the used analytical method – optical emission spectroscopy. Finally, the principles of rotational, vibrational and electron temperature calculations are described. The contemporary deposition process was carried out in continuous and pulsed mode of radiofrequently excited capacitively coupled discharge. The effects of monomer flow rate, plasma power and duty cycle on the deposition process were studied. In the individual spectra, atomic hydrogen lines of Balmer’s series as well as an atomic oxygen line were identified. Molecular bands of CO have been identified as Angstrom’s system and 3. positive system. In the case of the identified fragments, the intensity dependencies on the monomer concentration in mixture, plasma power and duty cycle were detected. Electron temperature of plasma was also calculated using the relative intensities of atomic hydrogen lines of Balmer’s series. Vibrational and rotational temperature could not have been determined because no suitable fragments for their determination were found in the spectrum. Based on findings mentioned above, partial composition of plasma and some of its properties were estimated. The subject of the further research will be determination of the exact content and structure of the thin films and investigation of other plasma characteristics. Plasma deposition is influenced by many factors, and the study of their optimal combination for the most efficient deposition process is a goal for the future research.
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Examination of the urogenital system Hospital Department of Radiology Jablonec nad Nisou p.o.
MENŠÍKOVÁ, Věra
The bachelor thesis named Examination of the urogenital system Radiodiagnostics Hospital Jablonec nad Nisou c. o. deals with methods of examination of the genitourinary system and creates a list and description of each method, using at these examinations. A radiology assistant is a non medical healthcare expert who uses their theoretical and practical knowledge gained during their university study in the fields of radiodiagnostics, radiotherapy and nuclear medicine. Radiology assistants are erroneously perceived both by other medical personnel and by lay public as technical workers. However, they are also medics who must be able to manipulate with highly sophisticated equipment but also provide medical care to the patients in full scale. As regards radiodiagnostics, the radiology assistants' task is to carry out the examination itself and obtain graphic documentation thereof. Radiology assistants ensure examination and handling of the equipment and they assist the doctor radiologist during more exacting intervention procedures. Radiology assistants' job is highly erudite and people working with ionising radiation must strictly abide by the laws and regulations stipulating strict rules and requirements which each radiology assistant must meet when they want to perform this activity. Radiology assistants' approach must help reduce the radiation burden of the population. It is logical, that the technological development goes hand in hand with higher examination yield and the ALARA principle is generally adhered to and applied in all examinations. If we strive to decrease the radiation dose, it is necessary to use an optimum amount of contrast agents. At the beginning of the thesis, there is a detailed description of urogenital system anatomy and physiology whose knowledge is a must when one wants to work in this field. Imaging methods described in the thesis range from the simplest ones to the most complex ones, both as regards the examination time and pursuant to specifications associated with individual examination types. The examination should be carried out from the least invasive examination procedures to the most complex ones. The methodics is based on gathering, study and subsequent analysis of the obtained data. Data from years 2009 2013 obtained at the radiodiagnostic ward of Nemocnice Jablonec nad Nisou p.o. hospital was gathered for this purpose. Investigations are divided into a number of men and women undergoing these tests. Investigative methods are compared, and evaluated that prevail, and from which they are abandoned prevail and which are being gradually abandoned. For the purpose of my thesi. The gathered data was processed into tables and synoptic graphs. Information from expert books, articles and verified Internet sources were used in the bachelor thesis. Results of my study confirmed the research question, the number of patients who undergo examinations of the urogenital system Radiodiagnostics Hospital Jablonec nad Nisou after rising. Preference tests are noninvasive and greater diagnostic yield. It is also found, however, that certain examination still have their place and are being replaced by another method. This example of hysterosalpingography. The aim of the thesis was to create an overview and description of individual radiodiagnostic methods in uropoetic system examinations and analysis of data from radio diagnostic department Hospital Jablonec nad Nisou regarding the examination of the urogenital system. The number of examinations in the radiodiagnostic ward is rising and the demands placed on a radiodiagnostic assistant are also continuously increasing from both physical and mental point of view. Radiology assistants are obliged to improve their knowledge and skills actively by means of training sessions and self study.
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Substituated Poly(ethylene Glycols) as Drugs Carriers
Pravda, Martin ; Hrabálek, Alexandr (advisor) ; Dittrich, Milan (referee) ; Kalendová, Andrea (referee)
The aim of this dissertation thesis was preparation of drug delivery systems for polyene antimycotics, especially amphtoricin B(AmB). Series of pH-sensitive conjugates of AmB with substituated poly(ethylene glycols) (PEG) have been synthesized and characterized for this purpose. The intermediate PEGs possess a 1,4-disubstituted benzene ring with aldehyde group at the end of the chain. The benzene ring is connected with PEG at its 4-position (with respect to the aldehyde group) by various functional groups (ether, amide, ester). Reaction of terminal aldehyde group of the substituted PEGs with AmB gave conjugates containinga pH-sensitive imine linkage, which can be presumed to exhibit antimycotic effect at sites with lowered pH value. The stability of prepared conjugates under the different physiological conditions was studied. Phosphate buffers (pH = 7,4 or 5,5) were used as model media. Stability of conjugates in human blood plasma and human blood serum was examinated. The imine linkage is split to give free AmB with half-lives of 2-45 min. The rate of acid catalysed hydrolysis depends upon substitution of the benzene ring; however, it does not depend on molecular weights ofthe PEGs used. The conjugates with ester linkage undergo enzymatic splitting in human blood plasma and/or blood serum at pH 7.4...
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