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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Information content in vocalization of guinea pigs (Cavia porcellus): individual distinction and recognition of predators
Baklová, Aneta ; Baranyiová, Eva (advisor) ; František, František (referee)
Guinea pigs represent domesticated precocious rodents which became common pets. From the first day after birth they are fully vocal. This thesis was devoted
to the vocalization of pups. The aim of the thesis was to 1) determine the age when the vocal individuality in whistle sound is demonstrable; 2) test possible ultrasonic signals emitted by young guinea pigs; 3) study the antipredator reactions and alarm calls to aerial (bird of prey) and terrestrial (dog) predators and human (control test).
A total of 16 guinea pig pups were tested for vocal individuality, 28 pups
for ultrasonic vocalization and 27 adolescents for predator recognition. When testing vocal ontogeny during the first 9 days after birth, I observed changes in temporal, frequency and parameter of intensity . When I test vocal individuality by cross-validated discriminant function analysis (DFA) based on ten acoustic parameters, calls were classified to correct animals with following success: day 1 = 71.9%, day 3 = 58.8%, day 5 = 53.10%, day 7 = 50.60% and day 9 = 63.10%. The highest frequency in whistle was 30.03 kHz. In predator discrimination reactions as freezing, fleeing and vigilance were observed. In the presence of dog, guinea pigs reacted for the longest time and most frequently by freezing. When confronted with a bird of prey, I observed for a longest time and most often fleeing and then freezing. When exposed to a human, guinea pigs showed mostly vigilance. Almost no vocalization was observed except for two events
of alarm calls - drrr as in the presence of dog and chirrup as reaction to bird of prey.
The following conclusions can be drawn from the presented results: 1) vocal individuality of guinea pigs is demonstrable immediately after birth and the rate
of individually different vocal parameters changes with age; 2) guinea pigs are able
to produce sound up to 30 kHz, i.e. within the ultrasound range, but signals of high frequencies are not crucial for their communication; 3) guinea pigs discriminate between terrestrial and aerial predators, but they emit alarm calls rather rarely.
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Corrosion behavior of plasma coatings CuAl10 and CuAl50 on magnesium alloy AZ 91
Kubatík, Tomáš František ; Stoulil, J. ; Stehlíková, K. ; Slepička, P. ; Janata, Marek
The most common magnesium alloy AZ 91 is widely used as a structural material, but its use is limited at higher temperatures and high humidity. Plasma spraying is a technology that allows to prepare protective metallic and non-metallic coatings on a wide variety of substrates including magnesium and its alloys. In this study, CuAl10 and CuAl50 were plasma sprayed on magnesium alloy AZ 91 with the aim to study corrosion resistance of the plasma sprayed coatings. The corrosion resistance of layers was evaluated by the method of electrochemical potentiodynamic measurement as well as long-term corrosion tests in a condensation chamber with 0.5 mol\nNaCl at the temperature of 35 °C for 1344 hours. Layers with 1, 2, 5 passes and passes of CuAl10 with the thickness ranging from 75 to 716 mm and CuAl50 with the thickness ranging from 64 to 566 mm were prepared. The increased corrosion velocity was observed in the case of thin layers of 2 and 5 passes due to the development of a galvanic corrosion couple. The CuAl10 layer prepared with ten passes has an outstanding corrosion resistance.
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Is genetic diversity congruent with morphological diversity across the distributional range of the Melampyrum subalpinum group (Orobanchaceae)?
CHLUMSKÝ, Jan
Allozymes were used to assess the genetic structure of 27 populations of Melampyrum subalpinum group and an artificial pollination experiment was carried out to examine the possibility of autogamy. Genetic variation was generally congruent with the known morphological variation of the group. The results corresponded with the central-marginal concept. Allelic enrichment due to hybridization with M. nemorosum was observed in some Austrian populations. Czech and Slovak populations do not differ from Austrian populations. The high inbreeding coefficient and the pollination experiment do not contradict the possibility of autogamy.
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Pick´s disease and the specifics of the nursing care of a patient with this disease
KOPKAŠOVÁ, Lenka
Pick's disease is a degenerative-atrophic process that primarily affects the frontal and temporal lobes of the brain. Besides Alzheimer's disease, it is a lesser known form of dementia which affects people of younger age. Although the exact range of Pick's disease occurrence varies in the literature, the average reported age is between 50-60 years. The disease can change eating habits, cause emotional swings, inappropriate behaviour and sloppy appearance of the patients. The behaviour to others is often selfish, the patients are unable to listen to others and ignore their surroundings. Pick's disease is also characterized by inappropriate sexual behaviour. Globally, the number of dementia patients is still growing and according to expert guesses the increase will continue. Nursing care for patients with dementia is therefore becoming a very actual topic as dementia is called a quiet epidemic of our century. Nurses in their departments quite frequently encounter patients suffering from dementia of various origins at different stages of disability and the staff at healthcare facilities often regard these people as a burden. The aim of this thesis, which is called Pick's Disease and Specifics of Nursing Care for Patients with this Disease, is, based on the theoretical knowledge, to map definitions and diagnosis of Pick's disease, to define the needs of such a patient, and to list the most common problems in the nursing care. It focuses on people with dementia within medical facilities. For proper understanding of the problem, the thesis starts with the anatomy and physiology of the brain and the description of cognitive functions which are affected by the dementia syndrome and the definition of dementia. Based on the studied literature, dementia is divided according to various criteria. It has been found out that dividing dementia is not entirely uniform, but the essential facts and overall opinions of the authors do not diverge. Pick's disease belongs to a group of frontotemporal dementia and its position in this group has changed over time, as it is recorded in the literature. The thesis includes recommendations resulting from the studied literature in the approach to patients with dementia and emphasizes personal communication with the patients and empathetic and humane approach. Although medicine cannot prevent dementia, an early diagnosis of a specific type of dementia and correctly targeted treatment can increase the quality of life not only of the patients but also of their families. I have chosen this topic because I find it very current and interesting. During my nursing practice at the Surgery Department and Aftercare Department, I have met patients suffering from dementia and I did not know how to properly and efficiently communicate with them. For the theoretical part of my work, the methods of explanation, synthesis and induction on the basis of Czech and foreign information sources have been used. The most frequently quoted Czech authors in my thesis are doc. MD. Roman Jirák, PhD., the head of the center for diagnosis and therapy of Alzheimer's disease at the psychiatric clinic of the General University Hospital in Prague, the 1st Medical Faculty of Charles University. This thesis can serve as an informational and educational source both for nurses and the general public who may experience the disease within their families. It would be considered a great success if it helped nurses or family members to diagnose Pick's disease or other dementia at an early stage.
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Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.
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Land Abandonment in the Mediterranean Effects on Butterfly Communities with Respect to Life History Traits
ŠLANCAROVÁ, Jana
This thesis deals with the effects of changing land use, following landscape abandonment, on butterfly communities in the Mediterranean Basin. It consists of three case studies. The first focuses on the effects of forest encroachment on butterflies in the Southern Balkans; the second studies butterfly communities in Portuguese 'montados' and the third explores demography and life histories of three co-occurring Papilionidae butterfly species (Archon apollinus, Zerynthia polyxena and Zerynthia cerisy) in Greek Thrace. The results describe shifts in butterfly communities, detectable even at the level of individual species life history traits, with increasing forest encroachment. The preference of range-restricted Mediterranean endemics for either grasslands or open woodland formations contributes to falsifying the forested Mediterranean hypothesis, favouring a hypothesis of finely grained landscape mosaic instead. This mosaic is currently threatened by land use change and biodiversity homogenisation. Maintaining habitat and landscape heterogeneity is crucial for conserving the Mediterranean biodiversity hot-spot.
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Tool to assess the landscape connectivity in different area units and dispersal distances
Chrumko, Adam ; Šímová, Petra (advisor) ; Krčílková, Šárka (referee)
The purpose of this diploma thesis is to introduce a basic summary of landscape connectivity calculations mainly focusing on the Conefor program and the effect of input parameters on output landscape connectivity indices. One of the main goals is to create an ArcMap Toolbox to simplify the calculation of landscape connectivity indices for many areas of interest. Basic squares that cover the whole Czech Republic were chosen as main areas of interest. These units are used for example for mapping the abundance of flora and fauna. The same indices were calculated for NUTS3 and LAU1 areas in order to compare effect of size of input areas. This thesis provides a simple solution in the form of a Python Toolbox for Esri ArcMap to calculate overall indices of landscape connectivity through a Conefor command line application for a large number of input areas. Forest connectivity for different dispersal distances and input areas of interest was calculated with this Toolbox. As an input vector layer of forests, CORINE land cover data from mapping period 1990, 2000, 2006 and 2012 were used. Input dispersal distances were chosen in the range from 50 to 50.000 meters in order to comprehensively describe their effects on indices. Resultant forest and connectivity changes are represented by cartograms; the effect of input data on output indices is shown in graphs.
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Comparison of trucks of selected power range
Effenberk, Jan ; Šařec, Petr (advisor) ; Balada, Ivo (referee)
The main purpose of this bachelor thesis is to compare semitrailer trucks made by major European manufacturers. Maximum engine power of compared vehicles is limited to 340 kW. Secondary aim of this thesis is provision of theoretical knowledge of key mechanisms and issues of modern trucks. The thesis is devided into two main parts. The first one includes history and development of road transport and trucks. Basic technical terms and legislation are described there as well. Following chapters are dedicated to brief characteristic of mechanisms and their types. The second part of this thesis focuses on truck comparison according to selected parameters using chosen method of multi-criteria analysis. The thesis is concluded with results evaluation, ranking of each truck and discussion about certain issues.
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