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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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Srovnání citlivosti 17 ekotoxikologických bioestů pro detekci cyanotoxinů
Maršálek, Blahoslav ; Bláha, L.
Detection potential of some bioassays used for cyanotoxin detection seems to be promising, but the real comparison of results and the sensitivity to cyanotoxins is imposible, because different authors use different cyanobacterial sample with uncomparable toxin composition, different methods for sample preparation, different design of bioassay, media, etc. A critical comparison of alternative bioassays for cyanotoxins detection, which is important for water management is up to date still missing. The aim of the present study was to compare the sensitivity of 17 bioassays for cyanotoxins detection (with respect to the content of hepatotoxic microcystins )using the same cyanobacterial biomass, and the same sample preparation . Additional aims of this study was as follow: i) can the fractionation of cyanobacterial biomass improve the sensitivity and decrease the variability of bioassays? , ii) which bioassay can more precisely dicrimine the presence of microcystins - is there any dose-response relationships? Cyanobacterial biomass was fractionated by SPE-C18 columns. Crude aqueous extracts, "non-toxic" pigment fraction without microcystins and "toxic" fractions (concentrated microcystins) were compared. This fractionation allowed to determine if the toxicity is due to microcystins, or some other not monitored metabolites present in the complex crude extract.
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Alternative methods of leading childbirth
PAPAI, Daria Kristina
The topic of my bachelor thesis is "Alternative methods of child delivery". The theoretical part describes the physiological delivery its particular phases and their progression. In addition, I aim to explain what the term alternative birth means, and what it includes. The various alternative positions are described, which can be used to facilitate childbirth, as well as their appropriate use during the particular delivery phases. The theoretical part is concluded by descriptions of pain relief methods, including the description of both pharmacological and non-pharmacological methods. The second part of the thesis is a practical part, summarizes the results of an evaluation research. The aim was to explore the most used alternative methods and to identify the views and experiences of women with alternative methods and aids used during delivery in selected hospitals. Furthermore, the aim was to describe the regions of the Czech Republic in terms of the use of alternative aids and procedures. The hypotheses were formulated based on previous experiences and personal opinions of the author of the thesis. The research sample consisted of women after a spontaneous child delivery in the České Budějovice Hospital and in the Znojmo Hospital. The research was conducted in the form of an anonymous questionnaire survey, using a non-standardized questionnaires. The results of the survey were compiled in tables and the chi-square test was applied as a method for statistical processing and verifying the hypotheses.
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Coping strategies in children´s fear and pain in relation to nursing care
PROCHÁZKOVÁ, Zuzana
For the nurse, taking care of hospitalized children is important not only pediatric nursing knowledge, personal and social preconditions, but also the theoretical and practical competency of methods and techniques, which are used for identification and effective influencing of pain and fear. The aim of this thesis was to identify the main sources of fear of the hospitalized preschool and young school age children and to find out the effective methods and principles of the cooperation with children, alternatively with their accompanying parents in order to moderate the fear of the small patient. Then the thesis tried to chart the nursing diagnostic and evaluation procedure of the pain and get the most used nursing methods of non-pharmacological pain relief. To obtain the necessary data, the qualitative research was used with a semi-structured interview as a research instrument. The first research group consisted of 12 nurses, the second group consisted of 12 children ´s patients of preschool and young school age. The majority of the asked nurses thinks, that children´s patients are afraid especially of non-familiar environment, then of the awaited pain and of the solitude without parents. However, the most patients said, that they were afraid especially of pain, then of non-familiar environment, restriction of their needs, hostile attitude of the medical staff and of the other neighbouring patients. They were also frightened of the death and of the darkness. Most reported nurses mean, that the most effective solution of the children ´s fear is the friendly attitude of the medics. They also mentioned the fact of taking the focus off the fear and the presence of the parents is also beneficial. The nurses ´ answers were in conformity with patients opinion in two fields - friendly attitude of the medics and taking the focus off the fear. Then the children mentioned abiding of their specifics in connection to falling asleep and the demand of beying treated by the same sister or by few same sisters. All respondents also talked about the benefit of parents presence. Regarding the principles of the cooperation, nurses answered the importance of united medical procedures, especially the proper way of information transfer. Then the respondents nurses mentioned the psychical and emotional support for children and their accompaniments, the atmosphere of confidence and the value of well done education. Also the need of calm, silence and privacy was stated. Unlike nurses, children and their parents regarding to principles of the cooperation prefer the psychical and emotional support, then the importance of enough information was mentioned. The fourth research question bears on the methods used for children pain monitoring. The nurses said, that the intentional interview with the patients or with the parents was used as a main technique, that was stated by patients as well. Research question number five said: What are the most used nursing methods of non-pharmacological pain relief at children? Most responded nurses mentioned the distraction of children attention from the painful incentive, then the importance of physical methods, relief position and purposeful preparation for the unpleasant intervention. Nurses also named the helpful contribution of the parents, relaxing exercises and the method of imagination. Regarding the pain treatment, it was noted, that all patients prefered the analgesic therapy. Then they quoted the distraction of their attention from the painful incentive, the friendly attitude of the medics, using the ice compress and the relief position. In the pain acute phase, children required mostly calm, comfort and privacy. All children also considered the presence of parents as very helpful. The theoretical and empirical part of the thesis are suitable for being studied by nurses taking care of children and it can be helpful for nursing quality improvement at children ´s wards in hospitals.
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Charity Projects of Organizations Providing Care for Children with Disabilities in South Bohemia
LANDOVÁ, Lenka
The bachelor thesis "Charity Projects of Organisations Providing Care for Children with Health Disability in the Region of South Bohemia" deals with those projects that were founded by non-profit organisations and that provide care for children with health disability in the Region of South Bohemia. The aim of this thesis is to chart the charity projects of above mentioned organisations and to find out what is a proper and effective way to create them and what methods and procedures are used by non-state, non-profit organisations. The first research inquiry refers to a successful implementation of these projects. The result will be not only a realisation what kind of projetcs were created by the above mentioned organisations but also what is important for an organisation itself. Within the bounds of the second research inquiry I find out wheather there is an optimal procedure for the implementation of the charity projects of non-state, non-profit organisations. A qualitative method was used for a research part of this thesis. I used a method of a half-structured interview, a technique of a personal interview. A research group was selected for bringing the research into practice, which includes nine organisations seleceted from non-state, non-profit organisations providing a care for health disabled children in the Region of South Bohemia. It is more difficult for non-state, non-profit organisations it this field to obtain enough finances for providing services and for obtaining necessary aids than for state non-profit organisations because of different ways of fundraising . A non-state, non-profit organisation has several possibilities how to obtain necessary material and non-material sources. There are charity projects that offer means of selfless help; provided they are successful, they help the organisation to obtain necessary sources. It emerged from the research that it was very important for non-state, non-profit organisations to get a multi-source fundraising and supporters of their projects, mainly donors and sponsors, to obtain available care for children with heath disability. On the basis of the research, it is possible to state that non-state, non-profit organisations providing care for children with health disability, implemented many successful projects. The results of this thesis can be used in the way of an informative material for setting a complete view of a solution of the problems of the above mentioned organisations projects.
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Political aspects of local government – (non)stability of voting behavior of population of the town Volary
Dvořák, Stanislav ; Kopřiva, Radek (advisor) ; Lenka, Lenka (referee)
The thesis analyses the elections and electoral behaviour of the citizens of the town Volary in the period from 1996 to 2014. The behaviour of the voters was studied not only from the election results of the municipal elections but parliamentary elections as well. Several theoretical propositions, including the consistency of the voters' social status were verified. Moreover the work describes the differences of electoral behaviour based on the division into various socioeconomic groups in connection with the left and the right wing of the political spectre. Furthermore the work reviews the theory whether the local voters tend to the left or right values and deals with the stability or instability of their electoral behaviour.
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The role of volunteering in NGOs working in the social sector
Tlach, Vladimír ; Varvažovská, Pavla (advisor) ; Pavel, Pavel (referee)
The thesis is thematically focused on the area of volunteering as a modern phenomenon occurring not only in the Czech Republic but in the whole world. The thesis is divided into theoretical and practical part.
The first, theoretical part, explains the basic theoretical background of the non-governmental sector with an emphasis on volunteer activity in existing non-governmental organisations. The theoretical part also presents the results of the researches of foreign and domestic experts on volunteering on which the research part of the thesis is based.
In the practical part of the thesis are analyzed two non-profit organisations - the Charity Ostrava and Slezská diakonie, which provide social services in the Moravian -Silesian region, and the company Arcelor Mittal, which is actively involved in the field of corporate volunteering. In the research part there are presented outcomes of the field research, which took place in the aforementioned non-profit organisations and the company, but also among public, where it was investigated what people know about non-governmental organisations in their close area, their opinion on volunteering and how they perceive volunteers. The outcomes from a field survey are then compared with foreign and domestic surveys.
The aim of the thesis is to propose possible options for changes in working with volunteers in the non-governmental organisations based on the findings, which could lead to streamlining of their work, an increase of the prestige of the volunteer work and a number of volunteers working in the social sector. Based on the investigation it was found that volunteering is beneficial to everyone involved. It allows an individual to actively participate in society, help others and spend free time in a meaningful way. The obtained findings help to improve volunteer work, identify potential reserves in the individual´s work and prevent possible conflicts.
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Creation of methodical process of inventory of property and commitments in a selected non-profit organization
Lorencová, Dana ; Štáfek, Pavel (advisor) ; Pavlína, Pavlína (referee)
The diploma thesis follows up a process of inventary in a chosen accountant unit, its found insufficiencies and its consecutive solution. The process of inventary belongs to activities that lead to completion of a closing financial statement of an organization and that is why all the actions and processes must be done with carefulness and accuracy according to law number 563/1991 of law collection about accountancy, which states, according to law of the European union, extent and way of managing the accountancy and all requirements for its conclusivness and conditions for handing it over to a state.
The theoretical part of the thesis is related to defining basic terms of inventory process. The valid law regulations, which are dealing with those issues, are stated there. Individual phases of the whole process, important intern regulations and documents, which are obligatory for every single accountant unit, are also stated in the theoretical part of the thesis. A continuity of the inventory of property and commitments in financial statements and statements is being described there.
The practical part of the thesis involves the process of inventory in a chosen non-profitable accountant unit of the district court in Jičín. In the individual phases of the inventory, when the physical and documentary stock-takings are being done, are found insufficiencies and proposals of their solutions. A complete process of inventory belongs to activities of the chosen accountant unit, on which state´s controlling institutions are focusing in recent years, because the activities involve economy of the state´s essets. A methodic instruction for the processing of inventory of property and commitments for an accountant unit is worked out in the last chapter of the practical part.
By the compilization of the diploma thesis a complete view on an inventory as a whole was given, which is a starting point for completion of a financial statement. While working out the thesis, rocess or system insufficiencies were analysed. These insufficiencies might cause that the accountant unit will not fullfill the § 6 paragraph 3 of law of accountancy, from which the compulsatory duty of inventory of property and commitments according to provision § 29 and § 30 of law of accountancy flows. The audit office can, on the basis of those insufficiences, proclaim that the inventory of property and commitments was not done correctly and can apply a sanction on the accountant unit. After the inventory process the accountant unit is compulsory to make an inventory report that is a substantial document together with financial statements, balance-sheet, profit and loss sheet and attachments to the authorization of the financial statements.
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Potential logging in stands outside the forest within Czech Republic
Ondřich, Libor ; Štícha, Václav (advisor) ; Šálek, Lubomír (referee)
This thesis deals with the potential of timber from forest stands outside the territory of the Czech Republic and the assessment itself non-forest areas. The work is divided into two parts. The first part includes research on non-forest woody vegetation, especially in the Czech Republic, the methodology for mapping the authors of these non-forest areas, and the results to which occurred at the end of this work. The second part contains a separate case study with its own mapping in selected areas of the Central Region. In conclusion then mentioned the part dealing with logging from areas outside forests, including data from selected companies and at the end of thesis of the mining potential of these areas.
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