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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Membrane potential measurement with voltage sensitive dyes
 Votavová, Barbora ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to realize measurements of membrane potential with voltagesensitive dye Di-4-ANEPPS and the data processed and analyzed. The work includes theoretical basis in the form of electrophysiology animal cells, explains fluorescence and describes the fluorescence microscope. The document is largely devoted to the characterization and distribution of voltage-sensitive dyes (VSD). The practical part deals with the various components necessary to perform the experiment as a pulse generator, high-speed camera and camera’s acquisition and describes experiment. The conclusion will be compared with results from theoretical assumptions.
Detailed record
Membrane potential measurement with voltage sensitive dyes
 Votavová, Barbora ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to realize measurements of membrane potential with voltagesensitive dye Di-4-ANEPPS and the data processed and analyzed. The work includes theoretical basis in the form of electrophysiology animal cells, explains fluorescence and describes the fluorescence microscope. The document is largely devoted to the characterization and distribution of voltage-sensitive dyes (VSD). The practical part deals with the various components necessary to perform the experiment as a pulse generator, high-speed camera and camera’s acquisition and describes experiment. The conclusion will be compared with results from theoretical assumptions.
Detailed record

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