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The use of negatve staining method for viral and bacterial diagnostics using the transmission electron microscope.
ŠOBEROVÁ, Tereza
This bachelor thesis is focused on the negative staining method used in making diagnosis of viruses and bacteria in the transmission electrom microscope (TEM). Theoretical part provides general description of TEM, negative staining method and basic characteristics of viruses used in this project. Detailed description of procedure designed for the preparation of specimens is the key part of this project. After their display in TEM, the testis analyses the quality of virus images and comparison of effects of the most common staining agents, pH, trehalose and different times of staining on displaying the ultrastructure of biological specimens and on the size of individual viral particles. The conclusion of project contains staining agents providing the best imaging of objects that have been selected based on the evaluated results and recommendations for potential enhancement of staining properties in other agents.
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Ecological and epidemiological aspects of tick-borne encephalitis
GREGOROVÁ, Eva
Tick-borne encephalitis (TBE) virus is endemic in many countries in central, eastern and northern Europe. Nowadays its importance rises because of its increasing incidence. This study focuses on characterization of tick-borne encephalitis virus, its host and human disease caused by this pathogen and efficacy of available vaccines. Furthermore, an evaluation of climatic changes and their influence on TBE incidence is discussed.
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Cultivation of tick-borne encephalitis virus in the presence of inhibitor of glycosylation: its effect on nucleotide sequence of genes encoding viral proteins.
FARKOVÁ, Karolína
In this work I compared nucleotide sequences of virus genes and the resulting aminoacid sequences of viral proteins of TBE virus variant Hypr3TM with the parental virus Hypr50/CB. This comparison serves for identification of nucleotide/aminoacid substitutions resulting for virus replication in an enviroment with inhibited protein glycosylation. I identified four nucleotide substitutions; three of them caused also changes in amino acid sequence. When compared to a low-passaged variant Hypr5, three of the nucleotide substituions were identical with this variant. One amino acid substitution is unique for variant Hypr3TM. This substitution caused changes in electrostatic potencial of receptor domain of protein E and thus can affect the protein-protein interactions within homodimers and homotrimers at the surface of the protein and the interaction of the E protein with host-cell receptors for the TBE virus.
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