National Repository of Grey Literature 29 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Příprava monoklonálních protilátek specifických proti antigenům viru klíšťové encefalitidy pro další využití v imunodetekci
ŠMÍDOVÁ, Hana
Monoclonal antibodies are immunoglobulins produced by a single clone of B cells and bind very specifically to a particular antigenic epitope. Hybridoma technology is used for their preparation and they are widely used for the treatment and diagnosis of many diseases. The aim of this study was to prepare monoclonal antibodies specific against tick-borne encephalitis virus antigens for further use in immunodetection, their characterization, and optimization of used detection methods.
Exprese proteinu NS5 viru klíšťové encefalitidy v lidských neurálních buňkách
JAKLOVÁ, Kateřina
This study focuses on the detection of tick-borne encephalitis virus (TBEV) NS5 protein in infected and NS5-transfected DAOY HTB-186 human neural cells. TBEV NS5 protein was shown to localize mainly on the membranes of the endoplasmic reticulum. An interesting finding was also nuclear localization, which is supported by the obtained data from both, confocal microscopy and subcellular fractionation.
Vliv klíštěcích slin na endoplazmatický stres v buňkách infikovaných virem klíšťové encefalitidy
SPĚVÁKOVÁ, Markéta
The aim of this study was to evaluate the effect of tick saliva on endoplasmic stress in PMJ2-R cells infected with tick-borne encephalitus virus. The endoplasmic stress was analysed through 3 different branches of unfolded protein response: ATF-6, PERK and IRE-1. Moreover, the thesis deals with the effect of tick salivary protein Iristatin on endoplasmic stress in infected cells.
Interakce viru klíšťové encefalitidy s myšími keratinocyty
KOCAROVÁ, Kristina
This thesis was focused on the infection of mouse keratinocytes with tick borne encephalitis. We examined the amount of virus in keratinocytes in different time points and we determined the influence of tick saliva derived from Ixodes ricinus on the infection.
Diferenciální exprese genů po infekci virem klíšťové encefalitidy u lidských primárních nervových buněk
OŠLEJŠKOVÁ, Petra
This study focuses on the differential expression of genes in the human primary neural cells after tick-borne encephalitis virus infection. Changes in gene expression were determined using qRT-PCR and compared by correlation analysis with results previously obtained by next generation sequencing. The difference in gene expression determined by qRT-PCR correlated with those obtained from the next generation sequencing.
Patogeneze a klinické aspekty infekce virem klíšťové encefalitidy
ELSTEROVÁ, Jana
This thesis contributes to knowledge about the combined impacts of the pathogenesis of the tick-borne encephalitis virus (TBEV) and the immunopathogenesis of the host on the clinical course of acute tick-borne encephalitis (TBE). The thesis further focuses on the process of TBEV neuroinvasion and the utilization of the host's immune products as potential therapeutic interventions.
Synthesis of new nucleosides as potential inhibitors of flaviviral replication
Horkelová, Simona ; Nencka, Radim (advisor) ; Janeba, Zlatko (referee)
Viruses of the Flaviviridae family are the causative agents of many dangerous diseases for which we currently have no known cure, and research into new drugs against them therefore represents one of the major challenges for modern medicinal chemistry. Targeting the proteins encoded by viruses is the most common approach to combat them. For flaviviruses, the non- structural protein NS5 exhibiting methyltransferase (MTase) and RNA-dependent-RNA polymerase (RdRp) enzyme activity appears to be one of the most suitable molecular targets. This bachelor thesis deals with the synthesis of new potential drugs capable of inhibition skill of flaviviral RdRp. C-nucleoside analogues were prepared, containing 2 types of heterocyclical base: 3-fluoropicolamide modified in positions 5 or 6 and pyrido[3,2-d]pyrimidine-4-amine modified in positions 6 or 7 using aryl or heteroaromatic substituent Key words: C-nucleosides, polymerase, flaviviruses, Tick-borne encephalitis virus, Suzuki reaction, Grignard reaction
Antiviral vaccination - tick borne encephalitis virus
Solarová, Pavlína ; Drda Morávková, Alena (advisor) ; Janštová, Vanda (referee)
Tick borne encephalitis is by one of the most severe disease of central nervous system. Agent of this disease is tick-borne encephalitis virus, which is transmitted mainly by tick. Clinical picture of TBE is considerably different and varied in individuals - from quite infection without symptoms up to severe cerebro-spinal meningitis, in such cases attacked persons by this virus leave hospital with whole-life handicap. We can prevent this disease by inoculation. We can see it in the neighboring Austria, where TBE almost became extinct because 90% of population is vaccinated. Vaccines are commercially available from 1979. Nevertheless only 17% population is vaccinated against TBEV. The reason for it could be fact that insurance companies do not provide the vaccines as well as not very effective health education. One of possibilities how we can this tendency reverse would be higher awareness of pupils at schools. In school educational programs there is contained prevention of viral disease, especially in general biology, biology of viruses and education to health.
Interakce viru klíšťové encefalitidy s cytoskeletem hostitelských buněk
PRANČLOVÁ, Veronika
This thesis is focused on the role of host cytoskeleton, primarily microtubules and microfilaments, during tick-borne encephalitis virus infection in human neuroblastoma cell line SK-N-SH and tick cell line IRE/CTVM19. The importance of cytoskeletal integrity and dynamics to the viral replication cycle were examined using specific chemical inhibitors showing the virus utilizes studied structures in both cell lines. Immunofluorescence microscopy revealed structural changes in the actin cytoskeleton during late infection in SK-N-SH cells. Moreover, differences in expression of cytoskeleton-associated genes in both cell lines were compared. Several genes with up-regulated expression in SK-N-SH cells were identified during late infection.
Vliv klíštěcích slin na replikaci viru klíšťové encefalitidy v myších makrofázích. Úloha interferonu-\recke{beta} a oxidu dusnatého.
BERÁNKOVÁ, Zuzana
The aim of this study was to characterize the replication of tick borne encephalitis virus in mouse macrophages and investigate the influence of tick saliva derived from Ixodes ricinus on the viral replication. Moreover, the effect of interferon (the member of type I interferons) and nitric oxide on virus replication was studied.

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