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Diagnostics of genom conditioned diseases with the use of micro- and nanoparticles
Mondeková, Věra ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
The bachelor´s thesis discusses possibilities of viral genome´s detection through use of biosensors, more specifically through use of magnetic particles. The introductory part consists of brief characteristic of viruses, mentioned as originator of genom conditioned diseases, followed by chapters related to selected methods of nucleic acid´s extraction and analysis. The main part is dedicated to magnetic particles. The practical part of thesis deals with possibility of use of biosensors in specific viral pathogen´s detection, selection of biocompatible molecules suitable for magnetic particle modification and description of specific DNA sequence isolation procedure through use of magnetic particles.
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Examination of polymorphisms in the IFITM3 gene using sequences and their clinical significance for the course of viral diseases.
TROJÁKOVÁ, Simona
Interferon Induced Transmembrane Protein 3 is an anti-inflammatory cytokine that belongs to the group of interferon-stimulated genes. The topology of the IFITM3 gene was clarified by the analysis of electron paramagnetic and nuclear magnetic resonance. The protein encoded by this gene induces immunity against influenza A virus and other viral diseases. It also disturbs the homeostasis of intracelular cholesterol, inhibits the entry of viruses into the cytoplasm of the host cells and inactivates new enveloped viruses originating from an infected cell. IFITM proteins reduce virus replication by regulating the expression of viral protein and by reducing the infectivity of developing viruses. The analysis of the single-nucleotid polymorphisms in the IFITM3 gene is very significant to clarify the mechanism of the effect of the IFITM3 protein and its influence on the severity of the course of the viral diseases. The theoretical part deals with the description of Interferon Induced Transmembrane Proteins, particularly the description and function of the IFITM3 gene, including its polymorphisms. This gene is located on chromosome 11 and its size is approximately 18 Kb. The gene variability of the IFITM3 can fundamentally affect the course of influenza and other viral diseases. The practical part of the thesis focuses on the detection of polymorphisms rs12252, rs34481144 and rs1136853 in the IFITM3 gene using the PCR method and Sanger sequencing method. It was necessary to master some basic laboratory methods such as DNA isolation from primary samples, PCR method, preparation of the PCR product for sequencing, sequencing data analysis, processing of results and determination of specific genotypes in tested individuals.
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Awareness of contagious disease in mind of pupils at basic and secondary schools.
Peštová, Ilona ; Rajsiglová, Ina (advisor) ; Fraiberk, Martin (referee)
Infectious diseases are a very hot topic nowadays in society. In recent years, there were several epidemics, infectious diseases (hepatitis A, Avian influenza, pandemic influenza A) and nobody knows when will the next "new" infection. At the outbreak of epidemics, there is great interest in the company to obtain information about the disease, but often also to unnecessary panic, because the media often publish incorrect information. It would be preferable, in order to improve public awareness and prevent the unnecessary spread of disease. Great emphasis should be given to prevent the disease - primarily on immunization, hygiene rules and principles of safe sex. Quality information should be mainly from teachers in teaching their pupils, as is clear from research books, infectious diseases are only mentioned in textbooks and the number of substantive information in them is missing. The fact that pupils of primary schools and grammar schools with basic information on infectious diseases do not meet in the classroom, evidenced by the results of a survey carried out in the 6th classes and first year at selected elementary schools and grammar schools in Prague. To raise awareness of the pupils in school was to create a methodical manual for teachers, which summarizes information about bacterial and...
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Parents' attitude to prevention of viral diseases in children
Šašková, Michaela ; Eislerová, Iva (advisor) ; Hrdličková, Renáta (referee)
Key words: parents, children, viral diseases, prevention, attitude Besides introduction and discussion and conclusion, the thesis consists of 3 chapters of theoretic part and the research part. The first chapter of theoretical part forms the general terms viruses and viral diseases. I have mentioned the biology of viruses and its characteristics. The second part of this chapter deals with the most frequent viral diseases in child age. I have described the ways of infection, diseases manifestation, possible complications and shortly the therapy too. The second chapter focuses on the immunity system of children. I have explained this issue generally at first, then I have described the function of immunity and later I have mentioned the development of immunity in children. At the end of the second part there is written about the most important factor of the future quality of immunity in children: the breast milk and breast-feeding. The third chapter deals with the most important topic of my thesis: prevention of viral diseases in children. Firstly I have described a term prevention in general. Then I have focused on the individual ways of prevention. At first I have mentioned the vaccination and then the other ways of prevention. In the research part of the thesis I have focused on my own research. I...
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Awareness of contagious disease in mind of pupils at basic and secondary schools.
Peštová, Ilona ; Rajsiglová, Ina (advisor) ; Fraiberk, Martin (referee)
Infectious diseases are a very hot topic nowadays in society. In recent years, there were several epidemics, infectious diseases (hepatitis A, Avian influenza, pandemic influenza A) and nobody knows when will the next "new" infection. At the outbreak of epidemics, there is great interest in the company to obtain information about the disease, but often also to unnecessary panic, because the media often publish incorrect information. It would be preferable, in order to improve public awareness and prevent the unnecessary spread of disease. Great emphasis should be given to prevent the disease - primarily on immunization, hygiene rules and principles of safe sex. Quality information should be mainly from teachers in teaching their pupils, as is clear from research books, infectious diseases are only mentioned in textbooks and the number of substantive information in them is missing. The fact that pupils of primary schools and grammar schools with basic information on infectious diseases do not meet in the classroom, evidenced by the results of a survey carried out in the 6th classes and first year at selected elementary schools and grammar schools in Prague. To raise awareness of the pupils in school was to create a methodical manual for teachers, which summarizes information about bacterial and...
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Possibilities in vaccination of dogs and cats
Čejková, Štěpánka ; Berka, Pavel (advisor) ; Daniel, Daniel (referee)
This bachelor thesis deals with the vaccination problems of domestic dog (Canis lupus familiaris) and domestic cat (Felis silvestris catus). The first part describes the basic information about the immune system to better understand formation of protection against infectious diseases in the form of memory cells, the principle of vaccination. Attention is also paid to the conditions to achieve the desired result of vaccination, including knowledge of the causative agent, the right technology of making vaccine, appropriate vaccination schedule, a suitable method of vaccine application and immunocompetent healthy individual, which will be able to react correctly to application of the vaccine. In veterinary medicine there are most commonly used vaccines with weakened or killed pathogens. Application is carried out mainly in the subcutaneous tissue or muscle, but the latest studies have shown significant benefit of application to the nasal cavity in connection with diseases affecting the respiratory tract. The primary vaccination of dogs and cats begins at their age of 8 to 12 weeks. It is necessary to carry out at least one revaccination at the age of 3 to 4 weeks after the primary vaccination. This revaccination serves as a stimulation of memory cells. In case of negative epidemiological situation two or even three applications can be made. Notwithstanding the fact that the memory cells in the body of an individual persist for a long time, yearly booster vaccination is still recommended for the majority of diseases. In the case of rabies, for some vaccines this revaccination is implemented once every three years. In the Czech Republic only the vaccination of dogs against rabies is required by law. Other diseases can be vaccinated optionally, depending on the disease situation, the use of dogs (hunting dogs, service etc.), and the possibility of travelling with a pet abroad. In connection with the vaccination, the basic information about infectious diseases of vaccinated dogs and cats are listed in this bachelor thesis.
As a part of this thesis a survey to find out awareness of dogs and cats´owners was carried out about the possibiliy of vaccination (and its use). These results are presented in graphs and provided in an appendix at the end of this bachelor thesis.
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Diagnostics of genom conditioned diseases with the use of micro- and nanoparticles
Mondeková, Věra ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
The bachelor´s thesis discusses possibilities of viral genome´s detection through use of biosensors, more specifically through use of magnetic particles. The introductory part consists of brief characteristic of viruses, mentioned as originator of genom conditioned diseases, followed by chapters related to selected methods of nucleic acid´s extraction and analysis. The main part is dedicated to magnetic particles. The practical part of thesis deals with possibility of use of biosensors in specific viral pathogen´s detection, selection of biocompatible molecules suitable for magnetic particle modification and description of specific DNA sequence isolation procedure through use of magnetic particles.
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