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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
PCR based assay for detection garden pea lec gene
 Vráblík, Aleš ; Hodek, Jan ; Ovesná, Jaroslava
The method was developed and verified by staff of National reference laboratory for GMO identification and DNA fingerprinting suited for governmental control laboratories and private laboratories that analyze food and feed derived from various plant matrices. Method can be applied for quantification of GM pea in a sample, when reference gene is used as a standard. The method describe internal garden pea specific gene, lektin in this case, detection using PCR and real-time PCR. The general principle of the assay relies on lektin specific sequence presence that represents a species specific gene of garden pea. In many matrices DNA is damaged so amplification of short stretches of DNA is used. After amplification of target exploiting newly developer species specific gene primers PCR products are separated by electrophoresis in agarose gel. Resulting band is visualized by UV light and its position is compare with size standard. Alternatively, real-time PCR and ABI platform in combination with SYBR® Green can be used. Reaction parameters are described and specificity of reaction was verified. LOD as well as LOQ was defined.
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Charlotta Garrigue Masaryková during the First World War
 Hájková, Dagmara
The article concentrates on the life of Charlotte Masaryk (1850-1923), wife of professor T.G. Masaryk, during the First World War, when her husband and daughter were in exile, her son Herbert died, her daughter Alice was imprisoned and her son Jan was in the Austrian army. The author describes the circumstances of T.G. Masaryk's departure for exile and the ways the family kept in touch. The relationship between Charlotta and Alice and health of Charlotte Masaryk are described. The article is based on the personal papers of Charlotte Masaryk and the family correspondence.
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