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Image inpainting
Vodička, Radek ; Špiřík, Jan (referee) ; Rajmic, Pavel (advisor)
The aim of this paper is to describe the basic principles of inpainting methods. We take a closer look with method of continual correction for damaged area from its margins in poins and by means of level lines. Result of the work is to compare images where inpainting was applied, among images themseves as well as with original image,and evaluation which method is the best in the given situation and for the given type of images.
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Monitoring the success of transfection of cell line 293 HEK
Dvořák, Tomáš ; Španová, Alena (referee) ; Ševčík, Mojmír (advisor)
Diploma thesis is based on monitoring the succes of transfection of cell linie HEK293. In theoretical part are described principles of transfection methods, cell lines, vectors and reporter genes. HEK293 cells EBNA1 were used for practical part. It was studied the difference between GFP and EGFP plasmids. As well as using various transfection reagents under different culture conditions.
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Elementary mathematics of imaging methods for radiology assistants
JELÍNKOVÁ, Barbora
The topic of the bachelor's thesis responded to the finding that there is currently no suitable educational text containing mathematical foundations for the education of radiological assistants and other related fields. The bachelor thesis contains selected areas of mathematics that are necessary to know to understand the physical foundations of imaging methods. The formulation of these areas is appropriate to the needs of future graduates of these fields of study. The goals of the bachelor thesis were the following: G1 Creation of an educational text and examples containing elementary mathematics that are essential to fully understand physical principles of imaging methods. G2 Compilation of a test that will be used to verify the knowledge of mathematics of radiological assistance field students and its graduates, followed by a statistical expression of knowledge level. Based on the goals, the following hypotheses were made: H1 By application of a curricular process a structure of elementary mathematics of imaging methods for radiological assistants can be described. H2a By comparing mathematical structures with components of a radiological assistant´s profile, individual structural levels of elementary mathematics for radiological assistants can be described. H2b According to needs and abilities of radiological assistants, examples of functions, differentiation, integration, and vectors can be chosen. H3 Knowledge of respondents in radiology mathematics will be distributed close to a normal distribution. With regard to the curricular process, a teaching text was compiled together with example illustrations. This step led to the fulfillment of goal G1. Then, a single-choice test of 20 questions was compiled to determine the level of mathematics knowledge across the field of radiological assistance. This step led to the achievement of goal G2. This test was subsequently extended using an online form among radiological assistants and students in this field. Given the above objectives, hypotheses H1, H2a and H2b could be confirmed. The test results were statistically expressed in the practical part of the bachelor thesis. It was confirmed that the empirical distribution of test responses is close to the normal distribution. Hypothesis H3 was also confirmed by this step. The benefits of the bachelor's thesis can be seen in the practical level (construction of an educational text verified by a test survey) and in the theoretical level (verification of the application of the theory of the curricular process).
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Detection and variation of the crayfish plague pathogen in selected crayfish populations
Mojžišová, Michaela ; Petrusek, Adam (advisor) ; Buřič, Miloš (referee)
Crayfish plague is an emerging disease caused by the oomycete Aphanomyces astaci, a pathogen listed among the 100 World's Worst Invasive Alien Species. It was introduced into Europe in the second half of 19th century from North America and caused collapses of European native crayfish populations. Nowadays, A. astaci is widespread in Europe and has spread also to other parts of the world, threatening all susceptible crayfish of non-North American origin. The aims of this MSc thesis were 1) to provide information about crayfish plague outbreaks from recent years, and by using microsatellite and mtDNA markers reveal A. astaci genotypes involved; 2) to test healthy-looking indigenous crayfish for potential occurrence of chronic infections by A. astaci in Czechia. Six new crayfish plague outbreaks were confirmed from 2016 to 2018, involving at least five distinct pathogen strains. My results provide first evidence of the A. astaci genotype group D causing Astacus astacus and Austropotamobius torrentium mass mortalities in Czechia. MtDNA sequencing revealed two haplotypes of the D haplogroup, indicating two independent sources of infection presumably either from ornamental crayfish or spreading from neighbouring countries. The genotype group A was recorded in two A. astacus mortalities and genotype group...
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Leishmaniases and their vectors in western Africa
Hošková, Anna ; Dvořák, Vít (advisor) ; Sádlová, Jovana (referee)
Leishmaniasis is a parasitic disease transmitted by sand flies. In West Africa, leishmaniasis is endemic and we distinguish cutaneous, visceral and mucocutaneous form of the disease. The most common form in this area is cutaneous leishmaniasis, which is caused by Leishmania major, Leishmania tropica and a new, as yet undescribed species Leishmania sp. from Ghana. The causative agents of visceral leishmaniasis are Leishmania donovani and Leishmania infantum. Phlebotomus duboscqi is a confirmed vector of L. major in this region. Vectors of Leishmania sp. from Ghana and vectors of visceral leishmaniasis remain unknown in West Africa. Sand flies of the genus Sergentomyia or Culicoides biting midges are considered as potential alternative vectors in this area. The confirmed reservoir hosts of L. major in some countries are the rodents Tatera gambiana, Arvicanthis niloticus and Mastomys erythroleucus, but in most states they are not confirmed. The reservoir hosts of visceral leishmaniasis are mainly dogs. Some West African states used to be French colonies, and there are many people of African descent in France who come to visit their relatives in West Africa. This poses a risk of import of leishmaniasis to non-endemic areas. The aim of this bachelor thesis is to summarize the current knowledge about the...
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Occurence and diversity of amphibian trypanosomes
Poloprutská, Klára ; Votýpka, Jan (advisor) ; Brzoňová, Jana (referee)
The genus Trypanosoma represents well-known flagellates of order Trypanosomatida and class Kinetoplastea, which includes over 500 described species parasitizing on all classes of vertebrates. However, the best known and economically and medically most important are mammalian trypanosomes, especially human ones, which cause serious diseases in the world: sleeping sickness in Africa and Chagas disease in Latin America. But trypanosomes may also be found in blood of birds, reptiles, fish and amphibians. And exactly on trypanosomes of amphibians, whose diversity is surprisingly vast, is this thesis focused. However, many studies concentrate only on morphological description, which may not be the best in order to determine species, which may lead to distortion of information e.g. about species' life cycle, prevalence or host specificity. This thesis points to some ambiguities, but also to some more reliable experimental or in some cases molecular evidence dealing with individual aspects of life of trypanosome of amphibians. It also summarizes representatives of aquatic and terrestrial invertebrate vectors of trypanosomes, among which leeches are considered to be the dominant vector in aquatic environment. Furthermore, some species of phlebotomes, mosquitoes and genus Corethrella are known to be...
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Detection and variation of the crayfish plague pathogen in selected crayfish populations
Mojžišová, Michaela ; Petrusek, Adam (advisor) ; Buřič, Miloš (referee)
Crayfish plague is an emerging disease caused by the oomycete Aphanomyces astaci, a pathogen listed among the 100 World's Worst Invasive Alien Species. It was introduced into Europe in the second half of 19th century from North America and caused collapses of European native crayfish populations. Nowadays, A. astaci is widespread in Europe and has spread also to other parts of the world, threatening all susceptible crayfish of non-North American origin. The aims of this MSc thesis were 1) to provide information about crayfish plague outbreaks from recent years, and by using microsatellite and mtDNA markers reveal A. astaci genotypes involved; 2) to test healthy-looking indigenous crayfish for potential occurrence of chronic infections by A. astaci in Czechia. Six new crayfish plague outbreaks were confirmed from 2016 to 2018, involving at least five distinct pathogen strains. My results provide first evidence of the A. astaci genotype group D causing Astacus astacus and Austropotamobius torrentium mass mortalities in Czechia. MtDNA sequencing revealed two haplotypes of the D haplogroup, indicating two independent sources of infection presumably either from ornamental crayfish or spreading from neighbouring countries. The genotype group A was recorded in two A. astacus mortalities and genotype group...
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Image inpainting
Vodička, Radek ; Špiřík, Jan (referee) ; Rajmic, Pavel (advisor)
The aim of this paper is to describe the basic principles of inpainting methods. We take a closer look with method of continual correction for damaged area from its margins in poins and by means of level lines. Result of the work is to compare images where inpainting was applied, among images themseves as well as with original image,and evaluation which method is the best in the given situation and for the given type of images.
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Monitoring the success of transfection of cell line 293 HEK
Dvořák, Tomáš ; Španová, Alena (referee) ; Ševčík, Mojmír (advisor)
Diploma thesis is based on monitoring the succes of transfection of cell linie HEK293. In theoretical part are described principles of transfection methods, cell lines, vectors and reporter genes. HEK293 cells EBNA1 were used for practical part. It was studied the difference between GFP and EGFP plasmids. As well as using various transfection reagents under different culture conditions.
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