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Vasodilatory effects of 4-methyl catechol: ex vivo mechanism of action
Kuzdřalová, Kateřina ; Pourová, Jana (advisor) ; Smutná, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Kateřina Kuzdřalová Supervisor: doc. PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: The mechanism of vasodilation effect of 4-methylcatechol ex vivo Keywords: rat, aorta, flavonoids, 4-methylcatechol, vascular smooth muscle, vasodilation Flavonoids belong to the group of polyphenolic bioactive substances found abundantly in plants, fruits and vegetables. Thanks to their pharmacological and biochemical effects, they play a crucial role for human health, especially in the prevention of numerous diseases - metabolic syndrome, osteoporosis, atherosclerosis and also diseases of a cardiovascular system. The aim of the diploma thesis was to determine the mechanism of the vasorelaxant effect of one of the most important metabolites of flavonoids - 4-methylcatechol. For the research we used a standardized ex vivo method on isolated Wistar rat aortic rings. We were able to confirm the vasodilatory effects of 4-methylcatechol. We also confirmed the fact that the flavonoid metabolite directly affects a vascular smooth muscle. Furthermore, we found that the tested compound potentiates the vasodilatory activity of sodium nitroprusside and forskolin, and that vasodilation depends on the activity of...
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Vasodilatory effects of 3-hydroxy phenylacetic acid: ex vivo mechanism of action
Štefancová, Monika ; Pourová, Jana (advisor) ; Vokřál, Ivan (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Monika Štefancová Supervisor: doc. PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: Vasodilatory effects of 3-hydroxy phenylacetic acid: ex vivo mechanism of action It is known from clinical studies that the intake of polyphenols from food acts as a prevention of cardiovascular diseases. However, the parent compounds themselves often have low bioavailability. The emphasis is therefore on their bioactive metabolites. 3-hydroxy phenylacetic acid (3-HPAA) is among such metabolites. The aim of this study was to verify the mechanism of the vasodilatory effect of 3-HPAA using standardized ex vivo method on an isolated pig coronary artery. The administration of 3-HPAA resulted in relaxation of maximally contracted segments of porcine artery by mechanism partially dependent on the integrity of endothelium. By inhibiting endothelial nitric oxide synthase, the relaxation was significantly impaired. The blockage of SKCa and IKCa channels, muscarinic receptors, cyclooxygenase or L-type calcium channels did not affect relaxation. Thus, 3-HPPA causes dose-dependent vasodilatation of coronary arteries ex vivo at least partially mediated by endothelium with the participation of nitric oxide. Key words:...
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Vasodilatory effects of 4-methyl catechol: ex vivo mechanism of action
Kuzdřalová, Kateřina ; Pourová, Jana (advisor) ; Smutná, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Kateřina Kuzdřalová Supervisor: doc. PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: The mechanism of vasodilation effect of 4-methylcatechol ex vivo Keywords: rat, aorta, flavonoids, 4-methylcatechol, vascular smooth muscle, vasodilation Flavonoids belong to the group of polyphenolic bioactive substances found abundantly in plants, fruits and vegetables. Thanks to their pharmacological and biochemical effects, they play a crucial role for human health, especially in the prevention of numerous diseases - metabolic syndrome, osteoporosis, atherosclerosis and also diseases of a cardiovascular system. The aim of the diploma thesis was to determine the mechanism of the vasorelaxant effect of one of the most important metabolites of flavonoids - 4-methylcatechol. For the research we used a standardized ex vivo method on isolated Wistar rat aortic rings. We were able to confirm the vasodilatory effects of 4-methylcatechol. We also confirmed the fact that the flavonoid metabolite directly affects a vascular smooth muscle. Furthermore, we found that the tested compound potentiates the vasodilatory activity of sodium nitroprusside and forskolin, and that vasodilation depends on the activity of...
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Vasodilatory effects of bisphenol AF ex vivo
Kuchařová, Nela ; Pourová, Jana (advisor) ; Vopršalová, Marie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Nela Kuchařová Supervisor: PharmDr. Jana Pourová, Ph.D. Title of diploma thesis: Vasodilatory effects of bisphenol AF ex vivo Bisphenols are organic compounds used in the manufacture of plastics, resins, varnishes and lot of other products. However, their effects on human body are associated with a number of adverse effect that need to be investigated in more detail. The aim of this diploma thesis was to determine whether bisphenol AF has vasodilating effects ex vivo and to verify the mechanism of the relaxing effect. The experiments were performed on an isolated thoracic aorta of a Wistar rat. Bisphenol AF was added cumulatively to the precontracted aorta at increasing concentrations and we monitored whether vasodilation was induced. In testing the mechanism of action, we used inhibitors of the mechanisms tested during the experiment. The obtained results were evaluated using the GraphPad Prism program. The results show that bisphenol AF has a dose-dependent ex vivo vasodilatory effect (EC50 = 57.16 μmol/l). The mechanism of this effect is the blockage of voltagegated calcium channels on the vascular smooth muscle cell. The participation of other tested mechanisms has not been confirmed. Key...
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