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The issue of blood donation from the perspective of doctors and the general public
Cicák, Filip ; Kordulová, Pavla (advisor) ; Kulhavá, Miluše (referee)
Introduction: The properties of human blood are so specific that it has not yet been possible to replace human blood with any other fluid than obtaining it as a blood gift from donors. In recent years, there has not been a sufficient number of new blood donors in the Czech Republic to compensate for the decrease in the number of donors due to the increasing incidence of a number of civilization diseases and age (Czech Red Cross, 2020). Blood donation and the need for blood products are constantly increasing with the development of the methods of treatment and medicine as a science. Ensuring a sufficient number of blood donors and sufficient deposition of blood obtained from donors is a permanent task of healthcare (Society for Transfusion Medicine of the Czech Medical Association JEP, 2019). Aim of the work: The main goal of the research was to map the issue of blood donation, to identify the factors by which blood donors are motivated to donate. Furthermore, what can cause stagnation in the growth of blood donors and what means doctors motivate potential blood donors. Methodology: A qualitative research method was chosen for the research survey. Data collection took place in the form of semi-structured interviews and was completed by achieving theoretical data saturation. In order to preserve...
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The Frequency of HLA Antigens in the Group of Plasma Donors of the Transfusion Department, University Hospital, Hradec Králové
Nováková, Petra ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Petra Novakova Title: The frequency of HLA antigens in the group of plasma donor of the Transfusion Departmen, University Hospital, Hradec Králové Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The major objective of this study is the statistical data processing in order to determine the frequency of HLA antigens in the group of plasma donors. Methods: The serological typing of HLA antigens using the lymphocytotoxic test was performed among plasma donors of the Transfusion Department of the University Hospital Hradec Kralove. On a sample group of 200 plasma donors was determined the frequency of HLA antigens by blood group and gender on the basis of a serological examination. The collected data of the observed sample of aforementioned group were processed using Microsoft Excel in relation to the gender and blood group of the donor. Results: It was discovered that within the observed sample there is the highest frequency of HLA-A antigens 2, 1 and 3; HLA-B antigens 7, 44, 35 and HLA-C antigens 7, 6, 4. The most frequented combination of HLA antigens within the observed sample of 200 plasma donors regardless blood group and gender are combinations of HLA-A 1,2 (15x) HLA-A 2,3 (15x), HLA-B 8,44 (8x), HLA-B 7,...
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Zpracování odpadů pocházejících z transfuzní stanice z hlediska mikrobiálních rizik
Novobilská, Markéta
The transfusion department at the hospital in Nový Jíčín is producing daily a large number of municipal and hazardous waste. Handling with such waste can cause injury or contamination of medical and other staff, donors and patients, but it can also couse unintetionall pollution or environmental damage. In the case of any personal injury it is necessary to follow the guidelines the prepared by the hospital and health ministry. The suspected injury are there tested on the viral infections (hepatitis B, C, or HIV) and/or the liver tests are provided. The aim of this diploma thesis is to describe the possible microbial hazards that are associated with hazardous medical waste, its production, but also with the subsequent handling. On a model experiment by using microbial tests was estimated the most likely focal point of possible infection. Evaluating of microbial risk assessment was determi-ned from the results. These results formed the basis for possible recommendations, which are summarized in conclusion.
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Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases
Lipenská, Zuzana ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Zuzana Lipenská Title: Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The aim of the study of HLA B27 antigen in patients investigated for suspected rheumatological disease in 2015. Methods: Patient samples examined for the presence of antigen HLA-B27 in the HLA laboratory of the Transfusion Department, University Hospital Hradec Kralove are processed using the serological method. First is used the isolation of peripheral blood lymphocytes using a density gradient, thereafter lymphotoxical test with peripheral lymphocytes. Results: In 2015 were examined 1,329 patients. 207 persons had the HLA-B27, 1122 persons did not. Of the total number of examined patients were examined 754 women and 575 men. Positive was found 108 women and 99 men. The highest incidence of HLA-B27 was observed in the group of 20-29 years old, another slight increase was in the group of 10-19 years old and 40-49 years old. The examined persons had mainly a group diagnosis of disease of the musculoskeletal system with the most frequently diagnosed dorsalgia (M54). Conclusions: The association between HLA-B27 antigen and the deseases has...
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