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Determination of residual substances from the preparation of colloidal systems based on biopolymers
Borůvková, Tereza ; Vávrová, Milada (referee) ; Mravec, Filip (advisor)
Volatile organic compounds could be isolated and concentrated from liquid solution using methods, which are based on analysis of equilibrium gas phase, because of their volatility. The aim of this bachelor´s thesis is determination the amount of residual tert-butanol, belonging to volatile organic compounds, in the samples after lyophilisation. Samples were prepared by mixing hydrofobic sonde and hydrofilic hyaluronan. For mixing these components it is necessary to add organic solvent tert-butanol, which ensures stable environment. Thanks to its presence mixing of probe and hyaluronan occurs. Consecutive lyopfilisation of the sample leads to the disruption of a water cover of hyaluronan and nonpolar hydrogen atoms, which are hidden in it, can interact with hydrofobic probe. Bonding the probe is important in oncology, where this fact could be used in targeted distribution of pharmaceuticals. However presence of tert-butanol is unacceptable in the final product, mainly in medical applications. The object this study is the minimization of residual tert-butanol amount in final lyophilised cake. The method of equilibrium gas phase analysis (static Head-Space) by gas chromatography with flame ionization detection (GC/FID) was used for the determination of its content.
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Determination of residual substances from the preparation of colloidal systems based on biopolymers
Borůvková, Tereza ; Vávrová, Milada (referee) ; Mravec, Filip (advisor)
Volatile organic compounds could be isolated and concentrated from liquid solution using methods, which are based on analysis of equilibrium gas phase, because of their volatility. The aim of this bachelor´s thesis is determination the amount of residual tert-butanol, belonging to volatile organic compounds, in the samples after lyophilisation. Samples were prepared by mixing hydrofobic sonde and hydrofilic hyaluronan. For mixing these components it is necessary to add organic solvent tert-butanol, which ensures stable environment. Thanks to its presence mixing of probe and hyaluronan occurs. Consecutive lyopfilisation of the sample leads to the disruption of a water cover of hyaluronan and nonpolar hydrogen atoms, which are hidden in it, can interact with hydrofobic probe. Bonding the probe is important in oncology, where this fact could be used in targeted distribution of pharmaceuticals. However presence of tert-butanol is unacceptable in the final product, mainly in medical applications. The object this study is the minimization of residual tert-butanol amount in final lyophilised cake. The method of equilibrium gas phase analysis (static Head-Space) by gas chromatography with flame ionization detection (GC/FID) was used for the determination of its content.
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