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Non-stacionary thermal field in the nozzle at stream mixing
Vosynková, Jana ; Vincour, Dušan (referee) ; Nekvasil, Richard (advisor)
Nuclear energy is already well known area of energy generation. However, it is necessary to eliminate the potential risks of failure. That places special emphasis on safety in production and operation of nuclear power, especially its primary circuit. This work is focused on thermal damage to the injection nozzle, which is part of the pressurizer in the primary circuit Dukovany plant.The aim was a comparative analysis of two geometries nozzle which were loaded by varying temperature loading trought time and by static preassure loading. Main attention was placed on the thermal shocks in the material.
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Influence of thermal shocks on fatigue of pressure equipment components
Umlauf, Martin ; Vincour, Dušan (referee) ; Létal, Tomáš (advisor)
This thesis examines influence of thermal shock on fatigue of pressure equipment components. The paper relates to project of IMI CCI Brno, which is a leading manufacturer of valves for the energy and process industries. Analysis concerns the impact of thermal shock concentrating on high pressure part of the steam valve. And proposal for a new valve geometry that reduces machining operations and reduce financial costs of production. First part of the thesis presents a basic review of fatigue damage components concerning mechanical loading and thermal – mechanical loading. Second part of the study creates FEM model of the valve. FEM model simulate temperature field and stress during thermal shock. Influence of thermal shock to fatigue damage was computed on basis of stress tensor obtained from FEM simulation under Chapter 18 of the standard EN 13445-3. Existing valve geometry was verified and the new geometry was proposed that simplified and reduce financial costs of production.
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The effect of Hsp70 protein on the infection caused by Potato virus Y
Doričová, Vlasta ; Hýsková, Veronika (advisor) ; Hoffmeisterová, Hana (referee)
Whithin their natural environment, plants are subjected to a combination of stress conditions. Since potential interactions between signal pathways, plants respond to multiple stresses differently from how they do to individual stresses, activating a specific programme. Heat shock proteins (HSP70) overexpressed after heat shock influence the viral infection. On one side HSP70 can participate on refolding of aggregated or partially denaturated proteins, on the other side HSP70 can interact with viral proteins and facilitate propagation of viral replication complexes. In this work the effect of heat shock (42řC, 2. hours) applied before or after the inoculation of plants Nicotiana tabacum L. cv. Petit Havana SR1 with Potato virus Y on viral infection was detected. This effect was studied in two biological experiments. The amount of coat protein of PVYNTN and protein HSP70 were detected simultaneously with the activity assays of Hatch-Slack cycle enzymes, glycosidases and peroxidase. Both experimental approaches (heat shock applied before or after the inoculation by PVYNTN ) enhanced amount of the virus and in the 2nd experiment it accelerated infection development. Immediately after application of heat shock the amount of HSP70 was increased. The enhancement of HSP70 by viral infection occurred...
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The effect of Hsp70 protein on the infection caused by Potato virus Y
Doričová, Vlasta ; Hýsková, Veronika (advisor) ; Hoffmeisterová, Hana (referee)
Whithin their natural environment, plants are subjected to a combination of stress conditions. Since potential interactions between signal pathways, plants respond to multiple stresses differently from how they do to individual stresses, activating a specific programme. Heat shock proteins (HSP70) overexpressed after heat shock influence the viral infection. On one side HSP70 can participate on refolding of aggregated or partially denaturated proteins, on the other side HSP70 can interact with viral proteins and facilitate propagation of viral replication complexes. In this work the effect of heat shock (42řC, 2. hours) applied before or after the inoculation of plants Nicotiana tabacum L. cv. Petit Havana SR1 with Potato virus Y on viral infection was detected. This effect was studied in two biological experiments. The amount of coat protein of PVYNTN and protein HSP70 were detected simultaneously with the activity assays of Hatch-Slack cycle enzymes, glycosidases and peroxidase. Both experimental approaches (heat shock applied before or after the inoculation by PVYNTN ) enhanced amount of the virus and in the 2nd experiment it accelerated infection development. Immediately after application of heat shock the amount of HSP70 was increased. The enhancement of HSP70 by viral infection occurred...
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The effect of heat shock stress on enzyme activities in plants.
Doričová, Vlasta ; Hýsková, Veronika (advisor) ; Liberda, Jiří (referee)
Heat stress is one of abiotic stress factors, which fundamentally influences the growth and development of plants. Plants response to heat stress by series of cell and metabolic changes, the specificity of heat stress is synthesis of molecular chaperons, called heat- shock proteins (HSP). The influence of heat shock (in the form of 1 hour application of 40řC from 20řC) on the activity of NADP-dependent enzymes, enzymes of Hatch-Slack cycle, glycosidases and the activity of peroxidase in tobacco plants (Nicotiana tabacum L. cv. Petit Havana SR1) was studied in this work. Since HSP are involved in proper folding of immature, misfolded or partly denaturated proteins, in degradation of denaturated proteins and in induction of thermotolerance of plants, the aim of this work was to find out, if the activity of studied enzymes will be maintained or modulated within the plant defense response. The highest amount of HSP70 detected immunochemically together with higher activities of NADP-malic enzyme, phosphoenolpyruvate carboxylase, β-hexosaminidase and α- mannosidase compared to control plants was found 1 hour after application of heat shock. Peroxidase activity was most increased 1 day after HS compared to controls. Using native electrophoresis the differences in isoform content between control and...
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Influence of thermal shocks on fatigue of pressure equipment components
Umlauf, Martin ; Vincour, Dušan (referee) ; Létal, Tomáš (advisor)
This thesis examines influence of thermal shock on fatigue of pressure equipment components. The paper relates to project of IMI CCI Brno, which is a leading manufacturer of valves for the energy and process industries. Analysis concerns the impact of thermal shock concentrating on high pressure part of the steam valve. And proposal for a new valve geometry that reduces machining operations and reduce financial costs of production. First part of the thesis presents a basic review of fatigue damage components concerning mechanical loading and thermal – mechanical loading. Second part of the study creates FEM model of the valve. FEM model simulate temperature field and stress during thermal shock. Influence of thermal shock to fatigue damage was computed on basis of stress tensor obtained from FEM simulation under Chapter 18 of the standard EN 13445-3. Existing valve geometry was verified and the new geometry was proposed that simplified and reduce financial costs of production.
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Non-stacionary thermal field in the nozzle at stream mixing
Vosynková, Jana ; Vincour, Dušan (referee) ; Nekvasil, Richard (advisor)
Nuclear energy is already well known area of energy generation. However, it is necessary to eliminate the potential risks of failure. That places special emphasis on safety in production and operation of nuclear power, especially its primary circuit. This work is focused on thermal damage to the injection nozzle, which is part of the pressurizer in the primary circuit Dukovany plant.The aim was a comparative analysis of two geometries nozzle which were loaded by varying temperature loading trought time and by static preassure loading. Main attention was placed on the thermal shocks in the material.
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