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Measurement of Spectral Characteristics of Fluorescent Voltage-Sensitive Dyes
Pavlík, Dušan ; Čmiel, Vratislav (referee) ; Provazník, Ivo (advisor)
This bachelor thesis deals with optical measurement of substances properties in practical service of absorption and fluorescent spectroscopy. The work specifies the principle of fluorescence, which has direct association with spectral characteristics measurement of so-called voltage-sensitive dyes (in this instance di-4-ANEPPS). This dye is used for contactless (optical) imaging of organisms electric activity in practical service of its voltage dependent spectral shift after binding itself on cell membrane. However, the right application of this fluorescent dye is dependent on too many factors – one of them, discussed and measured in this work, is di-4-ANEPPS spectral dependency on its immediate environment.
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Automation of the control of the optical measuring chamber
Šatka, Richard ; Benešl, Tomáš (referee) ; Bradáč, Zdeněk (advisor)
In this bachelor’s thesis, the problem of controlling the position of the rotary table in the optical measuring chamber and the measurement automations of the optical spectral characteristics using a spectrophotometer is described and solved. In frame of this bachelor’s thesis, a hardware and software solution was designed and implemented for controlling, calibrating and setting the position of the measuring chamber table. In addition, modifications of the software program for controlling the spectrophotometer and connecting the program with the service application for controlling the rotary table has been performed. The functionality of the system was verified by rotating the measuring table in the chamber to predefined positions, by measuring the optical emission spectrum of the halogen lamp and by measuring the optical reflection spectra of the sample with the hologram motives at various angles of light incidence on the hologram..
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Possibilities of utilization of nitrogen compounds from liquid suspensions generated in waste management
Vajdíková, Tereza ; Mergl, Václav (referee) ; Kalivoda, Josef (advisor)
This thesis deals with the removal of ammoniacal nitrogen from biogas plant waste to produce a new usable product. In the theoretical part of the thesis, attention is paid to waste management from the perspective of biogas plants, the problem of nitrogen, and its removal. The thesis contains, among other things, theoretical calculations that include distribution and precipitation curves. The practical part deals with the correctness of these curves, as well as the precipitation of ammonium ions to form ammonium magnesium phosphate and stripping. The practical part includes quantitative and qualitative analyses of the individual components involved in precipitation. Nitrogen removal was performed on the model and real samples.
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Monitoring jakosti vody vybraného toku
Zálešák, Petr
The bachelor thesis focuses on the evaluation of water quality in the Velička River. The Velička is located in the Hodonín district in southern Moravia. Its total length is 40.2 km. Its source is on the western slope of Velká Javořina and flows into the Morava River near Strážnice. The water flow was monitored in eight profiles at two-month intervals for one year. The monitoring had two phases. The first phase consisted of taking a water sample and measuring some of the water quality parameters in the field using an instrument kit from Hach Company. These were indicators of pH, conductivity, oxygen content, and water temperature. The second phase was carried out in a laboratory by measuring the quality parameters from the water samples using a spectrophotometer. The concentrations of total phosphorus, sulphates, nitrate nitrogen, and chlorides were monitored. The quality of the water in the river was evaluated according to the ČSN 75 7221 standard and government regulation No. 401/2015 Coll. Total phosphorus was evaluated as the most unfavorable qualitative parameter.
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Optical Characterization of Thin Films Using Evolutionary Techniques
Horáček, Miloslav ; Bidlo, Michal (referee) ; Jaroš, Jiří (advisor)
My master's thesis deals with creating of a suitable evaluation technique that optimizes the optical parameters of the thin films according to the specified requirements of the proposer. This technique will be used at the Faculty of Mechanical Engineering and it should facilitate the evaluation of measurement results of the The Institute of Physical Engineering workers. Research workers use a digital imaging spectrophotometer for some measurements of optical properties of thin films at the institute. My most important task is to process the output data of the spectrophotometer concerning selected thin film. Construed master's thesis describes all the tools and techniques that have been used for the implementation of the program including evolutionary techniques and basics of the thin films optics (within the framework of the electromagnetic optics). As an enclosure, an electronic medium with the source codes of the whole application is provided.
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Measurement of Spectral Characteristics of Fluorescent Voltage-Sensitive Dyes
Pavlík, Dušan ; Čmiel, Vratislav (referee) ; Provazník, Ivo (advisor)
This bachelor thesis deals with optical measurement of substances properties in practical service of absorption and fluorescent spectroscopy. The work specifies the principle of fluorescence, which has direct association with spectral characteristics measurement of so-called voltage-sensitive dyes (in this instance di-4-ANEPPS). This dye is used for contactless (optical) imaging of organisms electric activity in practical service of its voltage dependent spectral shift after binding itself on cell membrane. However, the right application of this fluorescent dye is dependent on too many factors – one of them, discussed and measured in this work, is di-4-ANEPPS spectral dependency on its immediate environment.
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