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Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
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Reprodukce slona afrického (Loxodonta africana) v Zoo Zlín - Lešná
Šimková, Daria
ŠIMKOVÁ, D. Reproduction of an African elephant (Loxodonta africana) in a Zoo Zlín – Lešná. Mendel University in Brno, 2019. Diploma thesis. The thesis analyzes the issue of reproduction of critically endangered species of African elephant. The work is focused on monitoring of animal weight and blood test results during the reproductive cycle. The first part describes the general characteristics of the reproduction cycle of the African elephant, the issue of pregnancy and childbirth, and the female reproductive system. In the second part, there are given data from three particular elephants from the Zoo in Zlín. The data are processed into graphs and these are then commented on and compared with the conclusions of already conducted studies and scientific papers. At the end, all the knowledge and forecast of the African elephant reproduction in the Zoo - Lešná in the future are evaluated. All the results show that females are fully capable of reproduction and there is no need to make any changes in the breeding of these animals.
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Social preferences and interactions of common eland during parturition period
Staňková, Helena ; Komárková, Martina (advisor) ; Fuchs, Roman (referee)
Common elands (Taurotragus oryx) belong between the world's biggest antelopes. The basic social unit is created by several females with offspring, males are joining them only in the breeding period. There is a dominance hierarchy in a herd, which eliminates strong negative conflicts mainly between adult females. Except agonistic interactions, affiliative ones are taken place as well, which have positive impact on relationships in a herd. The aim of this work is t o summarize previous studies dealing with social structure and behaviour of common eland focusing on affiliative, maternal and agonistic interaction in a herd. Experimental part is focused on preferences of females for a choice of the closest companion, if the choice is influenced with age, kinship or maternal state of a female. Data were collected through direct observations of a herd of common elands on a farm Lány using 15 min scans of chosen females. Distances were measured between females and all the other herd members. Selected social behaviour of any two members of a herd was recorded using an all occurrence sampling method. The statistical analysis showed that the most (98,77 %) of interactions were negative, females preferred contactless agonistic interactions, threat and withdrawal. Allogrooming was more often found between a...
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Sex determination of phenotypically undifferentiated bird species focused on the Long-eared Owl (Asio otus)
Hlasivcová, Kristýna ; Řezníček, Jan (advisor) ; Ehler, Edvard (referee)
The aim of the present thesis is to give a comparision on sex determination of phenotypically undifferentiated birds species focused on the Long-eared Owl (Asio otus). Compared to other species of owls is relatively a few researches on the Long-eared Owl and researches on sex differences have not led to the quantified results yet. Attention is focused on characteristics and mechanisms of birds coloration. This work includes the methods and results of colour perception and interpretation in sexual dimorphism. It focuses primarily on the differences in color of certain parts of the bird, such as the alula, secondary remiges and lower part of the wing. For those pieces is further evaluating color differences in sex and proposes solutions that could help in further researches on sex determination of the Long-eared Owl.
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Characterisation and regulation of muscarinic and adrenergic receptors Subtitle: The effect of stress on muscarinic and adrenergic receptors in the lung and in the heart
Nováková, Martina ; Mysliveček, Jaromír (advisor) ; Hynie, Sixtus (referee) ; Mravec, Boris (referee)
The aim of this thesis was to clarify the influence of the stress on the adrenergic and muscarinic receptors in the heart and in the lungs. Research was perform on rat hearts and lungs and on the hearts and lungs of the CRH KO mice. First, we assessed mRNA levels of all α- and β-adrenergic receptor and muscarinic receptor subtypes. Subsequently, we performed the radioligand-binding studies to determine densities of these receptors. We identified all three α1-adrenergic receptor subtypes in the rat lungs. In the lungs of WT mice, we found that the amount of α1-adrenergic and muscarinic receptors was sex-dependent. Densities of the former were higher in females and those of the latter were higher in males. There was no difference between males and females in β-adrenergic receptor density. As for CRH KO mice, the basal densities of studied receptors were lower than in CRH WT mice (except β1-adrenergic receptors in females). The main purpose of the thesis was to detect the immobilization-induced changes in the studied receptors in the kontrol (WT) and CRH KO mice. Short-term and long-term immobilization caused decrease in all α1-adrenergic receptor subtypes in females, whereas only α1A-adrenergic receptors decreased in males. The amount of β1-adrenergic receptors decreased in males and remained without...
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