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Isolation and detection of DNA from plant species important for food prodution
Orel, Matúš ; Rittich, Bohuslav (referee) ; Kovařík, Aleš (advisor)
In the food industry, it is very important to take care of the quality, safety and organoleptic properties of the products supplied. For this reason, food must be checked. However, not all information can be found using conventional techniques such as immunoassays, chromatographic techniques, etc. DNA-based techniques can be used for these cases where traditional procedures are insufficient. Among them, the best known technique is PCR. The aim of the thesis was to isolate DNA from vegetable samples (broccoli, beetroot, carrot and pepper). DNA was isolated using the magnetic particle method and the traditional CTAB method. Both methods were able to isolate the DNA from the vegetable samples in quality and at a concentration suitable for PCR, where the 35S rDNA gene region was amplified (more precisely about 700 bp of the 18S-ITS1-5,8S region). After amplification, the PCR products were subjected to restriction reactions and the results compared to bioinformatic analysis. These steps have succeeded in finding suitable enzymes for diferentiation of PCR products from the tested vegetable species.
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Leucocytozoon and methods of its detection in raptors
Sekerášová, Iveta ; Brzoňová, Jana (advisor) ; Majerová, Karolina (referee)
The genus Leucocytozoon is a bird parasite. Its hosts constitute usually of representatives of the Falconiformes group and hawks (Accipitridae). The vector of this parasite are blackflies (family Simuliidae). This thesis brings an overview of species of the genus Leucocytozoon so far detected in birds of prey. The thesis focuses primarily on methods of detecting Leucocytozoon in hosts. Traditionally, this genus has been described on the basis of its morphological characters observed via microscope. With the discovery of polymerase chain reaction method (PCR), the detection possibilities expanded and allow us to study this parasite on molecular levels. The thesis contains a basic overview of detection methods, characteristics of their principles and outlines their strengths and weaknesses regarding the practical applicability as well. Based on that, the microscopic approach alongside with the detection based on molecular methods is recommended.
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Isolation and detection of DNA from plant species important for food prodution
Orel, Matúš ; Rittich, Bohuslav (referee) ; Kovařík, Aleš (advisor)
In the food industry, it is very important to take care of the quality, safety and organoleptic properties of the products supplied. For this reason, food must be checked. However, not all information can be found using conventional techniques such as immunoassays, chromatographic techniques, etc. DNA-based techniques can be used for these cases where traditional procedures are insufficient. Among them, the best known technique is PCR. The aim of the thesis was to isolate DNA from vegetable samples (broccoli, beetroot, carrot and pepper). DNA was isolated using the magnetic particle method and the traditional CTAB method. Both methods were able to isolate the DNA from the vegetable samples in quality and at a concentration suitable for PCR, where the 35S rDNA gene region was amplified (more precisely about 700 bp of the 18S-ITS1-5,8S region). After amplification, the PCR products were subjected to restriction reactions and the results compared to bioinformatic analysis. These steps have succeeded in finding suitable enzymes for diferentiation of PCR products from the tested vegetable species.
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Leucocytozoon and methods of its detection in raptors
Sekerášová, Iveta ; Brzoňová, Jana (advisor) ; Majerová, Karolina (referee)
The genus Leucocytozoon is a bird parasite. Its hosts constitute usually of representatives of the Falconiformes group and hawks (Accipitridae). The vector of this parasite are blackflies (family Simuliidae). This thesis brings an overview of species of the genus Leucocytozoon so far detected in birds of prey. The thesis focuses primarily on methods of detecting Leucocytozoon in hosts. Traditionally, this genus has been described on the basis of its morphological characters observed via microscope. With the discovery of polymerase chain reaction method (PCR), the detection possibilities expanded and allow us to study this parasite on molecular levels. The thesis contains a basic overview of detection methods, characteristics of their principles and outlines their strengths and weaknesses regarding the practical applicability as well. Based on that, the microscopic approach alongside with the detection based on molecular methods is recommended.
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