National Repository of Grey Literature 6 records found  Search took 0.00 seconds. 
Speciation genomics in nightingales
Mořkovský, Libor ; Reifová, Radka (advisor) ; Macholán, Miloš (referee) ; Piálek, Lubomír (referee)
Speciationisusuallyaslowprocessoccurringoverthousandstomillionsofyears.Thismakes speciation research difficult because no direct observation or manipulation is possible. At best, we can gain some insight by inferring the population history and structure in very fine detail by investigating genetic markers in multiple individuals of the nascent species. Today, speciationresearchisinanunprecedentedpositionthankstotheadventofhigh-throughput sequencingmethods,whichmakeiteasier and cheaper than ever before to evaluate multiple markers in many individuals. Speciation is not a straightforward process that happens in the same way every time, but rather a phenomenon occurring when genetic and ecological circumstancesactinginsymphonyultimatelyleadtoreproductiveisolationoftwosubpopula- tions. This is why it is important to study multiple model systems to understand the general principles behind speciation. We worked with two species of nightingales (Luscinia luscinia andL.megarhynchos)thatdivergedapproximately1.8Mya,likelyduetoglacialfluctuations in Europe. Our main goal was to use these new high-throughput sequencing methods to (1) detect interspecific hybrids between the species, (2) estimate levels of interspecific gene flow,(3)findareasofthenightingalegenomethatunderliereproductiveisolationand,finally, (4)...
Speciation genomics in nightingales
Mořkovský, Libor
Speciationisusuallyaslowprocessoccurringoverthousandstomillionsofyears.Thismakes speciation research difficult because no direct observation or manipulation is possible. At best, we can gain some insight by inferring the population history and structure in very fine detail by investigating genetic markers in multiple individuals of the nascent species. Today, speciationresearchisinanunprecedentedpositionthankstotheadventofhigh-throughput sequencingmethods,whichmakeiteasier and cheaper than ever before to evaluate multiple markers in many individuals. Speciation is not a straightforward process that happens in the same way every time, but rather a phenomenon occurring when genetic and ecological circumstancesactinginsymphonyultimatelyleadtoreproductiveisolationoftwosubpopula- tions. This is why it is important to study multiple model systems to understand the general principles behind speciation. We worked with two species of nightingales (Luscinia luscinia andL.megarhynchos)thatdivergedapproximately1.8Mya,likelyduetoglacialfluctuations in Europe. Our main goal was to use these new high-throughput sequencing methods to (1) detect interspecific hybrids between the species, (2) estimate levels of interspecific gene flow,(3)findareasofthenightingalegenomethatunderliereproductiveisolationand,finally, (4)...
Triploid plants: barriers or mediators of gene flow in mixed-ploidy species?
Voltrová, Alena ; Kolář, Filip (advisor) ; Hojka, Jakub (referee)
The aim of this work is to review our knowledge on the evolutionary significance of triploid plants, modes of their origin and mechanisms preventing triploid formation, and to answer the question of whether triploids act rather as mediators or barriers in gene flow between populations possessing different numbers of chromosome sets (diploid and tetraploid). The production of unreduced gametes is considered to be the major pathway of triploid formation in otherwise diploid populations, following the merger of haploid and diploid gamete. In contrast, in mixed di-tetraploid populations, triploids are usually formed by interploidy hybridization. The frequency of formation and fitness of triploids is reduced by a number of reproductive barriers, both pre- and postzygotic, the most important of which is the so-called triploid block caused by an unbalanced ratio of originally maternal and paternal genes in the nourishing tissue endosperm. Although the formation of triploids is accompanied by many barriers, which also further translate to their reduced viability, followed by additional reduction in fertility due to meiotic problems, triploids play a more important role in populations than might be expected solely on the basis of their rare occurrence in nature. Triploids act as mediators of gene flow...
Speciation genomics in nightingales
Mořkovský, Libor ; Reifová, Radka (advisor) ; Macholán, Miloš (referee) ; Piálek, Lubomír (referee)
Speciationisusuallyaslowprocessoccurringoverthousandstomillionsofyears.Thismakes speciation research difficult because no direct observation or manipulation is possible. At best, we can gain some insight by inferring the population history and structure in very fine detail by investigating genetic markers in multiple individuals of the nascent species. Today, speciationresearchisinanunprecedentedpositionthankstotheadventofhigh-throughput sequencingmethods,whichmakeiteasier and cheaper than ever before to evaluate multiple markers in many individuals. Speciation is not a straightforward process that happens in the same way every time, but rather a phenomenon occurring when genetic and ecological circumstancesactinginsymphonyultimatelyleadtoreproductiveisolationoftwosubpopula- tions. This is why it is important to study multiple model systems to understand the general principles behind speciation. We worked with two species of nightingales (Luscinia luscinia andL.megarhynchos)thatdivergedapproximately1.8Mya,likelyduetoglacialfluctuations in Europe. Our main goal was to use these new high-throughput sequencing methods to (1) detect interspecific hybrids between the species, (2) estimate levels of interspecific gene flow,(3)findareasofthenightingalegenomethatunderliereproductiveisolationand,finally, (4)...
Sperm morphology in the secondary contact zone of Common Nightingale and Thrush Nightingale
Opletalová, Kamila ; Reifová, Radka (advisor) ; Piálek, Jaroslav (referee)
The male gametes (sperms) are under strong sexual selection and are therefore very diverse in their morphology and often differ even amongst closely related species. Sperms are thus assumed to play very important role in reproductive isolation between species, due to their fast evolution in morphology. In my master thesis, I have studied the possible role of sperm morphology divergence in reproductive isolation in two sister species of passerine birds, the common nightingale (Luscinia megarhynchos) and the thrush nightingale (L. luscinia). The areas of these species overlap in secondary contact zone running across central and Eastern Europe, where they occasionally hybridize. I have compared sperm morphology of males of both species originating in allopatric and sympatric localities as well as interspecies hybrids. The results showed significant differences in total sperm length which is approximately 20 % longer in the common nightingale. That is caused by great interspecies divergence in midpiece (containing mitochondria) length. Interspecific hybrids showed sperms with intermediate length but despite expectations completely morphologically normal. This outcome corresponds with observed fertility in F1 hybrid males. What I consider to be an essential finding is a significant divergence in head...
Origin of species by interspecific hybridization in animals
Štiková, Jana ; Reifová, Radka (advisor) ; Choleva, Lukáš (referee)
Speciation mostly occur by splitting an ancestral species into two daughter species. Alternatively, new species may arise by hybridization, a phenomenon known as hybrid speciation. This type of speciation is frequent especially in plants, but recently, a growing number of example has been described also in animals. The aim of this thesis is to summarize the current knowledge about hybrid speciation and describe known examples of hybrid speciation in animals. Hybrid speciation in animals is mostly homoploid, e.g. without the change of number of chromosomes. I found 23 homoploid hybrid species in animals. Most of them belong to insects and fishes, but know examples are also among birds and mammals. Origin of species in animals by hybrid speciation seems to be more commons than was previously thought. Powered by TCPDF (www.tcpdf.org)

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