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Synthesis, characterization and study of the properties of functional antibody conjugates
Stránská, Andrea ; Hrubý, Martin (advisor) ; John, Jan (referee)
Radioimmunotherapy combines biological treatment with the use of highly effective radioactive radiation, which allows for more effective and gentle cancer therapy. This bachelor thesis focuses on the use of the therapeutic radioisotope 161Tb, which is characterized by the emission of low-energy electrons and gamma rays that allow monitoring of therapy and targeting of cancer cells, metastases and whole tumors. This theranostic approach offers increased treatment efficacy, especially in the early stages of the disease. This thesis focuses on a model study of rituximab monoclonal antibody conjugates that have been fluorescently labelled using a fluorescein tag. Covalent binding between the immunotherapeutic conjugate and the fluorescent label was accomplished using free amino groups of lysines and bioorthogonal chemistry. A biologically active antibody conjugate with a fluorescent label was developed that allowed direct monitoring of the conjugate binding to CD20-expressing cells using confocal microscopy. Subsequently, a radioactive conjugate of rituximab, modified with the p-SCN-Bn-CHX-A"-DTPA chelator and radiolabeled with the 161 Tb radioisotope, was tested. The biological activity of the radioimmunoconjugate was determined by in vitro experiments. Biodistribution of the radioimmunoconjugate was...
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Radiolabelled antibodies - the perspective for diagnosis and therapy
Mejtská, Jana ; Bárta, Pavel (advisor) ; Ramos Mandíková, Jana (referee)
1 CHARLES UNIVERSITY FAKULTY OF PHARMACY IN HRADEC KRÁLOVÉ DEPARTMENT OF BIOPHYSICS AND PHYSICAL CHEMISTRY DISSERTATION THESIS RADIOLABELED ANTIBODIES - THE PERSPECTIVE FOR DIAGNOSIS AND THERAPY Supervizor: Mgr. PAVEL BÁRTA, Ph.D. HRADEC KRÁLOVÉ, 2017 Bc. JANA MEJTSKÁ 2 ABSTRACT EN Different types of tissues have a characteristic cell morphology. Each cell has typical molecules on its surface, which may be either of physiological or pathological type. The presence of these surface structures can be interesting for possible modulation of specific cell populations from neighboring cells. Utilization of this property is then essential particularly in a case of tumor cells. Targeting on tumor specific cell structures involves the use of receptor specific peptides or monoclonal antibodies. The discovery of the preparation of monoclonal antibodies has opened a new chapter in the treatment and diagnosis not only tumor diseases. The advantage of monoclonal antibodies is their specificity and also high affinity to the type of the target cell structures. This study is focused on the summary of monoclonal antibodies which are currently being applied on the treatment or diagnosis of a particular cancer. Furthermore, this work also includes antibodies which are under development for intended medical applications with...
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