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Detekce nukleových bazí pomocí kapilární elektroforézy
Hendrychová, Kateřina
Capillary electrophoresis (CE) is one of the most important techniques used for high-throughput separations in modern analytical chemistry. Its main advantages include the possibility of using different detection techniques, speed and efficiency of separation as well as reproducibility. These properties are used in the separation of a wide variety of analytes ranging from inorganic ions to nanoparticles and were also verified in this work, in which the basic building blocks of DNA and RNA, nucleic bases, which serve as biomarkers for many disorders, diseases and food safety, were measured using capillary electrophoresis with a UV detector. The bachelor thesis is focused on the development and optimization of a method to detect and separate 5 nucleobases and purine. The separation was achieved by using 40 mM borate buffer pH 9,8 with the addition of 60 mM SDS as a detergent which forms micelles.
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Dichotomy in regioselectivity of Pd-catalyzed direct C-H arylation of protected uracils
Čerňová, Miroslava ; Hocek, Michal
Uracil bases and nucleosides bearing aryl groups in positions 5 or 6 are an important class of compounds and display wide range of biological activities1. In addition, arylation in position 5 is often used for labeling of nucleotides, oligonucleotides and DNA for applications in bioanalysis or chemical biology2. The 5- or 6-aryluracils can be prepared by heterocyclization or by cross-coupling reactions of halouracils with arylboronic acids or stannanes or metallated uracils with aryl halides. Direct C–H arylation of uracil is an alternative to classical cross-couplings where the preparation of reactive organometallic reagent is avoided. Recently, we have developed regioselective Pd-catalyzed and/or Cu-mediated direct C–H arylations of 1,3-dimethyluracil as a model compound for pyrimidine bases to position C-5 or C-6 3.
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The optimized microwave-assisted decomposition of formamides and its synthetic utility in the amination of purines and pyrimidines
Čechová, Lucie ; Jansa, Petr ; Šála, Michal ; Dračínský, Martin ; Holý, Antonín ; Janeba, Zlatko
The microwave-assisted decomposition of DMF was thoroughly studied and the reaction conditions (temperature, solvent effect, and effect of additives such as acids, bases, and salts) were optimized for its use in the amination reactions of various purines and pyrimidines.
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