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System with AC generator for cell electroporation
Lukoianov, Petr ; Folprecht, Martin (referee) ; Červinka, Dalibor (advisor)
This bachelor thesis is observing a system with AC generator for cell electroporation. The first paragraph keeps an eye on description of methodology of biophysics mechanisms. In the 2nd part several kinds of devices were described. In the last paragraph you can see a non-assembled construction and operating principle of the device. Apart from that the control circuit logic explored and device manual is included.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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Adjustable source feeded and controlled by USB bus
Staněk, Miroslav ; Frýza, Tomáš (referee) ; Kolouch, Jaromír (advisor)
The aim of this bachelor’s thesis is concept and realization of voltage source which is controlled and feeded by Universal Serial Bus. There will be presented whole design, execution, development progress and finalization of this project in the following text. This source must boost and buck standard voltage from USB. So it will be necessary using two source lines. Microcontroller of AVR family will be used in the direct part of this adjustable source. Into this microcontroller will be embedded USB interface by the software solution. The microcontroller isn‘t used only for communication with PC by the computer programme, but it is also used for switching the source lines by the PWM modulation. According to the minimum output voltage scale (0 – 15 V) this source is useful for a wide spectrum of low- power devices. This equipment could be helpful together with laptop to charging devices (cell phone, camera, pocket osciloscope etc.), which are carried with notebook for doing outdoor activities and measurements.
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Inverter for arc-welding
Vaculík, Josef ; Hejkrlík, Jan (referee) ; Červinka, Dalibor (advisor)
This bachelor project deals with the explanation of working principle of pulse source used as the source of welding current. It explains the activity of used single acting transmit converter and mention the others examples of converters types. Next there are mentioned powerful constructed parts of source, so input filter, pulse transformer, output inductor and powerful semiconductors components together with necessary parts converter. There are mentioned output current sensor and boards of powerful and directive parts of converter.
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System with AC generator for cell electroporation
Lukoianov, Petr ; Folprecht, Martin (referee) ; Červinka, Dalibor (advisor)
This bachelor thesis is observing a system with AC generator for cell electroporation. The first paragraph keeps an eye on description of methodology of biophysics mechanisms. In the 2nd part several kinds of devices were described. In the last paragraph you can see a non-assembled construction and operating principle of the device. Apart from that the control circuit logic explored and device manual is included.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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Adjustable source feeded and controlled by USB bus
Staněk, Miroslav ; Frýza, Tomáš (referee) ; Kolouch, Jaromír (advisor)
The aim of this bachelor’s thesis is concept and realization of voltage source which is controlled and feeded by Universal Serial Bus. There will be presented whole design, execution, development progress and finalization of this project in the following text. This source must boost and buck standard voltage from USB. So it will be necessary using two source lines. Microcontroller of AVR family will be used in the direct part of this adjustable source. Into this microcontroller will be embedded USB interface by the software solution. The microcontroller isn‘t used only for communication with PC by the computer programme, but it is also used for switching the source lines by the PWM modulation. According to the minimum output voltage scale (0 – 15 V) this source is useful for a wide spectrum of low- power devices. This equipment could be helpful together with laptop to charging devices (cell phone, camera, pocket osciloscope etc.), which are carried with notebook for doing outdoor activities and measurements.
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Inverter for arc-welding
Vaculík, Josef ; Hejkrlík, Jan (referee) ; Červinka, Dalibor (advisor)
This bachelor project deals with the explanation of working principle of pulse source used as the source of welding current. It explains the activity of used single acting transmit converter and mention the others examples of converters types. Next there are mentioned powerful constructed parts of source, so input filter, pulse transformer, output inductor and powerful semiconductors components together with necessary parts converter. There are mentioned output current sensor and boards of powerful and directive parts of converter.
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