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The impact of small phenolic metabolites of flavonoids (propionic acids) on the iron-catalysed Fenton reaction
Spiller, Sára ; Lomozová, Zuzana (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosis and Pharmaceutical Botany Candidate: Sára Spiller Supervisor: PharmDr. Zuzana Lomozová, Ph.D. Title of diploma thesis: The impact of small phenolic metabolites of flavonoids (propionic acids) on the iron-catalysed Fenton reaction Keywords: iron, flavonoid, Fenton reaction, antioxidant, pro-oxidant Iron is an essential element, necessary for the proper functioning of the human body. It is involved in many cellular processes where it acts as a cofactor. It is physiologically very important, especially for its ability to readily accept and deliver electrons. Its amount in the body is strictly regulated because in excess of the free metal, harmful radical reactions and the development of oxidative stress could result. Oxidative stress is responsible for many pathological processes. Flavonoids are natural polyphenolic substances that are found in many plants. Their consumption is associated with beneficial effects on human health, including antioxidant, anti-inflammatory, and cardioprotective properties. Despite their beneficial effects, they may, under certain circumstances, possess pro-oxidant effects related to their redox properties. During the metabolism of flavonoids, the gut microbiome degrades them to simple...
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The impact of benzoic acids, metabolites of flavonoids, on the iron-catalysed Fenton reaction
Klenovičová, Alexandra ; Mladěnka, Přemysl (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Alexandra Klenovičová Supervisor: Prof. Přemysl Mladěnka, PharmDr., Ph.D. Consultant: Zuzana Lomozová, Ph.D. Title of diploma thesis: The impact of benzoic acids, metabolites of flavonoids, on the iron-catalysed Fenton reaction Oxidative stress is a condition in which there is an increased production of free radicals that exceeds endogenous antioxidant activity and leads to damage of biomolecules. A number of human diseases are associated with oxidative stress. However the use of antioxidants has failed in clinical trials, but it is possible that the fact that antioxidants may preferentially exhibit prooxidant activity under elevated antioxidant concentrations in various pathophysiological conditions. Various in vitro and epidemiological studies have confirmed the presence of antioxidant and prooxidant properties in flavonoids. However, on the basis of their pharmacokinetics, it is evident that after their oral intake, significant metabolism in the colon takes place, where small phenolic substances are formed in interaction with the intestinal microflora. There is a possibility that the antioxidant and harmful prooxidant properties that have been attributed to flavonoids are in fact a...
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The influence of benzoic acids (metabolites of flavonoids) on the copper-triggered Fenton reaction and hemolysis
Harčárová, Patrícia ; Lomozová, Zuzana (advisor) ; Karlíčková, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacognosis and Pharmaceutical Botany Student: Patrícia Harčárová Supervisor: Zuzana Lomozová, MSc., Ph.D. Title of diploma thesis: Effect of benzoic acids (flavonoid metabolites) on copper catalyzed Fenton reaction and hemolysis Keywords: flavonoids, benzoic acids, copper, Fenton reaction, hemolysis Copper is a biogenic trace element that is present in several enzymes and proteins in the human body. The ability of copper to change its oxidation state is essential for its biological functions and enables enzymatic redox reactions in the organism. Homeostasis of copper is strictly regulated in the human body as its disruption can lead to the toxic effects of copper. Flavonoids are secondary plant metabolites that are a common part of our diet and their consumption has positive effects on our bodies. However, under certain conditions, they can also have a pro-oxidative effect, which is related to their ability to reduce transition metal ions. Flavonoids are massively metabolized in the human body before reaching systemic circulation into small phenolic substances including benzoic acids. Seven benzoic acid derivatives were tested as part of this thesis. Their influence on the production of hydroxyl radicals arising during the...
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