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Production and characterization of novel aryl sulfotransferases and their use in bioanalytics
Petránková, Barbora ; Bojarová, Pavla (advisor) ; Křížek, Tomáš (referee)
Polyphenols are the most common natural substances found in food. The largest group is represented by flavonoids, phenolic acids and catechols. Polyphenols are metabolized in the body during the biotransformation phase II, they can be sulfated, glucuronylated or methylated. To study biotransformation, it is necessary to have defined standards. Sulfated polyphenols can be prepared by enzymatic sulfation, which needs much milder reaction conditions compared to chemical sulfation. Prokaryotic sulfotransferases use 3'-phosphoadenosine-5'-phosphosulfate (PAPS) as a sulfate donor for sulfation, which is very expensive and difficult to regenerate. An alternative is the group of bacterial aryl sulfotransferases (EC 2.8.2.22), which catalyze the transfer of a sulfate group from a phenolic donor to a phenolic acceptor. Two bacterial aryl sulfotransferases from Desulfitobacterium hafniense and Escherichia coli have been described in the literature so far. The present thesis deals with the production, purification and biochemical characterization of two new bacterial aryl-sulfotransferases from Desulfosporosinus sp. HMP52 and Salmonella bongori. The enzymes were heterologously expressed in E. coli and purified by affinity chromatography. Their pH and temperature optimum, stability in organic solvents and...
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Phenolic complex of medicinal plants
Vaníčková, Klára ; Orsák, Matyáš (advisor) ; Hniličková, Helena (referee)
This thesis deals with medicinal plants, that contain phenolic complex. Emphasis on interdisciplinary relations with the chemistry and the use of theory in practical life. At the beginning, I also focused on the concept of health and lifestyle, which is an inherent part of that topic. Content is also the use of medicinal plants, basic plant description and important characteristic of the phenolics contained in plants. At the theoretical part is processed an overview of selected kinds of medicinal plants to draw attention to the content of the substance, especially with the phenolic complex special effects and their possible use.
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