|
|
Role of Renal functional reserve (RFR) in developing and recovering from cisplatin-induced renal injury
Hromadová, Pavlína ; Pávek, Petr (advisor) ; Trejtnar, František (referee)
University in Salamanca Faculty of Pharmacy Toxicology Area, Department of physiology and pharmacology Candidate: Pavlína Hromadová Supervisor: Prof. Rosa Laura Vicente, Ph.D. Prof. PharmDr. Petr Pávek, Ph.D. Title of diploma thesis: Role of RFR in developing and recovering from cisplatin-induced renal injury The purpose of this study was to find out the role of renal functional reserve (RFR) during development and recovery from cisplatin renal damage. This work focuses on evaluating whether RFR can detect (stages) of kidney damage that are not diagnosed with the clinical reference biomarker: plasma creatinine. To evaluate the RFR, Wistar rats produced in the animal house of the University of Salamanca were used. A protocol was established in which an intravenous perfusion of the amino acid glycine was used as a stressor and the glomerular filtration rate was evaluated through creatinine clearance. The animals received a toxic dose of cisplatin and the RFR was determined in one group two days before the maximum point of toxicity (day 2 of the experiment, evolution of kidney damage), in another one on the day of maximum toxicity (day 4) and in the last group on day 11 (recovery from kidney damage) after drug administration on day 0. The values are compared with those of a control group not treated...
|
|
|
Laboratory diagnostics of kidney injury in patients with diabates mellitus I and II.
Múčková, Ľubica ; Dršata, Jaroslav (advisor) ; Tichý, Aleš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Ľubica Múčková Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: Ing. Jana Netriová, PhD. Title of diploma thesis: Laboratory diagnostics of kidney injury in patients with diabetes mellitus I and II Diabetes mellitus is a chronic metabolic disease. This disease leads to develompement of both microvascular and macrovascular complications. In this work we examined a microvascular complication - diabetic nephropathy - and its laboratory diagnostics. We focussed on parameters cystatin C and creatinine. We estimated a glomerular filtration with Grubb, Stevens and MDRD equations. The Grubb and Stevens equations use cystatin C to determine the glomerular fitrlation rate (GFR), MDRD equations use creatinine, albumin and urea. For the asessment of kidney function we evaluated albumin and total protein waste in urine samples collected for 24 hours. The parameters were measured in file of 183 patients. Number of pacients with diabetes I.type was 49 and with diabetes II.type was 134. The main intention of this work was to evaluate equations used for determination of GFR, to compare their pros and cons and put them in contrast with albumin and total proteins waste. In case of the patients...
|
|
|
Laboratory diagnostics of kidney injury in patients with diabates mellitus I and II.
Múčková, Ľubica ; Dršata, Jaroslav (advisor) ; Tichý, Aleš (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Ľubica Múčková Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: Ing. Jana Netriová, PhD. Title of diploma thesis: Laboratory diagnostics of kidney injury in patients with diabetes mellitus I and II Diabetes mellitus is a chronic metabolic disease. This disease leads to develompement of both microvascular and macrovascular complications. In this work we examined a microvascular complication - diabetic nephropathy - and its laboratory diagnostics. We focussed on parameters cystatin C and creatinine. We estimated a glomerular filtration with Grubb, Stevens and MDRD equations. The Grubb and Stevens equations use cystatin C to determine the glomerular fitrlation rate (GFR), MDRD equations use creatinine, albumin and urea. For the asessment of kidney function we evaluated albumin and total protein waste in urine samples collected for 24 hours. The parameters were measured in file of 183 patients. Number of pacients with diabetes I.type was 49 and with diabetes II.type was 134. The main intention of this work was to evaluate equations used for determination of GFR, to compare their pros and cons and put them in contrast with albumin and total proteins waste. In case of the patients...
|
|
|
Analysis of urine after dynamic load with mean attention on tubular markers
Hrstková, Vladěna ; Procházka, Michal (advisor) ; Bitnar, Petr (referee)
The main focus of our research was to compare changes in ion excretion in urine, osmolality, indicators of exercise-induced proteinuria and tubular markers before and after dynamic exercise on a bicycle ergometer. The test sample consisted of 7 healthy, physically active women aged 27.4 years ( σ = 3.82 ). For dynamic exercise we uniformly set parameters 65 % VO2max and duration of load in 50 minutes. We compared the laboratory results of urine samples taken just before the exercise with urinary samples that we have collected immediately after exercise. The results of our measurements showed a significant changes in exercise-induced urinary sodium ions, creatinine and phosphorus. Statistically insignificant changes excretion was found between markers: chlorine, urine, osmolality, and N-acetyl-beta- d-glucosaminidase. These markers, however, show a trend the significance. Statistically insignificant changes after exercise have shown excretion of urinary markers: potassium, gelatinase-associated lipocalin, neutrophil microalbuminuria and alpha-1-microglobulin. Tubular markers of kidney damage after exercise is not at the reference values, that would indicate the tubular kidney damage.
|
guest ::
