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Fluorescence probes to study the properties of hydrogel systems
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This bachelors thesis is focused on possibility of selected fluorescent probes for study of properties of hydrogel systems. Chosen probes are Prodan for polarity study, HPTS for measurement of pH of the probe environment and platinum complex [2,6-Bis(2-pyridyl)fenyl-C,N,N]chloroplatinum(II) for its unique fluorescence properties and potential of energy transfer studies in hydrogel systems. Spectral properties of fluorophores were defined in selected set of solvents with different polarity with steady state fluorescence spectroscopy method. Properties of HPTS probe were also tested in buffers with different pH. Subsequently, hydrogels based on hyaluronan-septonex and dextran-SDS with incorporated fluorescence probes were prepared. Steady state fluorescence measurement of fluorophores from hydrogel were made using fluorimeter optical fibres. It was found, that probes Prodan and HPTS are suitable for study of polarity and pH of hydrogels and probably they will be able to be used for study of hydrogel with other techniques, like fluorescence microscopy, however platinum complex is, due to the low fluorescence intensity and possible quenching by polar water environment, probably not suitable for other measurements.
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Fluorescence probes to study the properties of hydrogel systems
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This bachelors thesis is focused on possibility of selected fluorescent probes for study of properties of hydrogel systems. Chosen probes are Prodan for polarity study, HPTS for measurement of pH of the probe environment and platinum complex [2,6-Bis(2-pyridyl)fenyl-C,N,N]chloroplatinum(II) for its unique fluorescence properties and potential of energy transfer studies in hydrogel systems. Spectral properties of fluorophores were defined in selected set of solvents with different polarity with steady state fluorescence spectroscopy method. Properties of HPTS probe were also tested in buffers with different pH. Subsequently, hydrogels based on hyaluronan-septonex and dextran-SDS with incorporated fluorescence probes were prepared. Steady state fluorescence measurement of fluorophores from hydrogel were made using fluorimeter optical fibres. It was found, that probes Prodan and HPTS are suitable for study of polarity and pH of hydrogels and probably they will be able to be used for study of hydrogel with other techniques, like fluorescence microscopy, however platinum complex is, due to the low fluorescence intensity and possible quenching by polar water environment, probably not suitable for other measurements.
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Získávání empirických energií základních stavů pomocí teplotní analýzy spekter z dusíkem chlazené kyvety a superosonické trysky
Mašát, M. ; Votava, Ondřej
In this paper we presented a method for determination the lower state energies, and hence the rotational quantum number, in the overtone spectra of methane (the Icosad polyad, between 7076 to 7291 cm-1). The method explores the temperature dependence of rovibrational transition intensities. In the first part of this report this approach is used with spectra measured at a) 81 K in liquid nitrogen cooled cell [Kassi et al., 2008] and b) 25 K in supersonic jet expansion. In the second part we demonstrate how the temperature-dependent line intensities can be directly extracted from the pulsed supersonic expansion experiment.
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