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Immobilization of pectinase on selected carriers
Reichstädter, Marek ; Zichová, Miroslava (referee) ; Omelková, Jiřina (advisor)
The theoretical part of this bachelor thesis deals with the definition of pectin substances as substrates for pectolytic enzymes. It also describes the changes in properties of enzymes due to enzyme immobilization, various methods of immobilization and application of immobilized enzymes. The experimental part examines the preparation by immobilization of commercially used pectolytic complex Rohament P by sorption on the polymeric carrier made of polyethylene tereftalate (PETE) bottles, what is a new patent of BUT. The main observed property was the enzyme activity of all preparations, it was determinated by Somogyi method and spectrometry – for both free and immobilized enzymes in dependence on the quantity of immobilized enzyme adsorbed onto specific weight of carries. The difference in effects change between the free and immobilized enzyme by measuring the viscosity decrease of the substrate depending on the degradation of glycosidic bonds was also studied.
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Screening of extremozyme production of selected extremophilic PHA producers
Dyagilev, Dmitry ; Obruča, Stanislav (referee) ; Pernicová, Iva (advisor)
This bachelor thesis deals with the screening of the production of extracellular hydrolytic enzymes in thermophilic microorganisms of the genera Aneurinibacillus, Brevibacillus, Chelatococcus, Pseudomonas, Schlegelella, Tepidimonas and Caldimonas. The ability of selected enzymes, namely proteases, lipases, amylases, xylanases, cellulases and pectinases, was tested in the investigated microorganisms. Such testing made it possible to assess in which microorganisms the production of specific enzymes can be observed. Based on the results of the screening, it was found that Schlegelella aquatica LMG 23380, Tepidimonas fonticaldi LMG 26746 and the investigated microorganisms of the genus Chelatococcus did not show the ability to produce any of the tested enzymes extracellularly. In natural isolates of Brevibacillus borstelensis LK 99 and Aneurinibacillus thermoaerophilus LK 102, only the ability to produce lipolytic enzymes was detected. The isolate Brevibacillus borstelensis Bz acts as a universal producer of all selected extremozymes. Enzyme activity was determined for selected producers. The bacterium Brevibacillus borstelensis Bz proved the ability to produce all six selected hydrolytic enzymes and has the highest activity of lipases, xylanases, cellulases and pectinases from the tested microorganisms. The highest proteolytic activity was measured in Thermomonas hydrothermalis DSM 14834 when cultured on skimmed milk powder.
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Screening of extremozyme production of selected extremophilic PHA producers
Dyagilev, Dmitry ; Obruča, Stanislav (referee) ; Pernicová, Iva (advisor)
This bachelor thesis deals with the screening of the production of extracellular hydrolytic enzymes in thermophilic microorganisms of the genera Aneurinibacillus, Brevibacillus, Chelatococcus, Pseudomonas, Schlegelella, Tepidimonas and Caldimonas. The ability of selected enzymes, namely proteases, lipases, amylases, xylanases, cellulases and pectinases, was tested in the investigated microorganisms. Such testing made it possible to assess in which microorganisms the production of specific enzymes can be observed. Based on the results of the screening, it was found that Schlegelella aquatica LMG 23380, Tepidimonas fonticaldi LMG 26746 and the investigated microorganisms of the genus Chelatococcus did not show the ability to produce any of the tested enzymes extracellularly. In natural isolates of Brevibacillus borstelensis LK 99 and Aneurinibacillus thermoaerophilus LK 102, only the ability to produce lipolytic enzymes was detected. The isolate Brevibacillus borstelensis Bz acts as a universal producer of all selected extremozymes. Enzyme activity was determined for selected producers. The bacterium Brevibacillus borstelensis Bz proved the ability to produce all six selected hydrolytic enzymes and has the highest activity of lipases, xylanases, cellulases and pectinases from the tested microorganisms. The highest proteolytic activity was measured in Thermomonas hydrothermalis DSM 14834 when cultured on skimmed milk powder.
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Immobilization of pectinase on selected carriers
Reichstädter, Marek ; Zichová, Miroslava (referee) ; Omelková, Jiřina (advisor)
The theoretical part of this bachelor thesis deals with the definition of pectin substances as substrates for pectolytic enzymes. It also describes the changes in properties of enzymes due to enzyme immobilization, various methods of immobilization and application of immobilized enzymes. The experimental part examines the preparation by immobilization of commercially used pectolytic complex Rohament P by sorption on the polymeric carrier made of polyethylene tereftalate (PETE) bottles, what is a new patent of BUT. The main observed property was the enzyme activity of all preparations, it was determinated by Somogyi method and spectrometry – for both free and immobilized enzymes in dependence on the quantity of immobilized enzyme adsorbed onto specific weight of carries. The difference in effects change between the free and immobilized enzyme by measuring the viscosity decrease of the substrate depending on the degradation of glycosidic bonds was also studied.
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