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Inducible expression systems and their use in the study of parasitic organisms.
Horáčková, Vendula ; Doležal, Pavel (advisor) ; Fišer, Radovan (referee)
1 Abstract Inducible expression systems are systems with ability to switch expression of genes of interest on and off. Therefore, they are useful molecular tools for analysis of gene function. Nowadays, there are tens of various inducible expression systems available that differ from each other in level of regulation of gene expression, time of induction, possibilities of use, etc. This work is focused on three of them to illustrate common features of the inducible expression systems which regulate gene expression at the level of transcription. Firstly, systems based on regulation of lactose operon of Escherichia coli are mentioned. Secondly, systems which use regulatory elements of tetracycline resistance-encoding transposon Tn10 of E. coli are described. Third chapter is focused on systems regulated by agonists of ecdysone receptor. In the last chapter cases of use of inducible expression systems in the study of parasitic organisms are summarized.
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Current chemotherapy of protozoal parasitoses.
Valach, Marek ; Hrdý, Ivan (advisor) ; Arbon, Dominik (referee)
This work is devoted to the process of chemotherapy of diseases caused by parasitic protozoa. Even though being often shadowed by bacterial or viral infections, protozoan caused diseases affect hundreds of millions of people every year, especially in tropical and subtropical regions of the developing world. This great burden placed on inhabitants of these countries highlights the need for functional chemotherapeutics against these diseases, because vaccination is not yet an option. The goal of successful chemotherapy is either elimination or suppression of the parasite in the patient by exploiting its weaknesses in its life cycle or differences in physiology between the parasite and the host. The aim of this work is to provide an overview of chemotherapeutics currently approved for the treatment four most prevalent parasite diseases. The diseases are clearly described in the terms of symptoms, their socioeconomical impact and lifecycle of the parasite. An emphasis is placed on the description of mode of action of each chemotherapeutical agent and on mechanism allowing the parasite to resist the drug.
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Inducible expression systems and their use in the study of parasitic organisms.
Horáčková, Vendula ; Doležal, Pavel (advisor) ; Fišer, Radovan (referee)
1 Abstract Inducible expression systems are systems with ability to switch expression of genes of interest on and off. Therefore, they are useful molecular tools for analysis of gene function. Nowadays, there are tens of various inducible expression systems available that differ from each other in level of regulation of gene expression, time of induction, possibilities of use, etc. This work is focused on three of them to illustrate common features of the inducible expression systems which regulate gene expression at the level of transcription. Firstly, systems based on regulation of lactose operon of Escherichia coli are mentioned. Secondly, systems which use regulatory elements of tetracycline resistance-encoding transposon Tn10 of E. coli are described. Third chapter is focused on systems regulated by agonists of ecdysone receptor. In the last chapter cases of use of inducible expression systems in the study of parasitic organisms are summarized.
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