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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Isolation of proteins from sow oviductal fluid by affinity chromatography on DNA cellulose
 Černá, Tereza ; Liberda, Jiří (advisor) ; Ryšlavá, Helena (referee)
Oviductal fluid was isolated from sow oviduct and then frozen and lyophilized. The obtained material was separated by affinity chromatography on DNA-cellulose and used as well for determination of antimicrobial and glycosidases activity. Two fractions - non-binding and bound - were obtained by affinity chromatography on DNA-cellulose. Four protein zones were detected on the SDS-electrophoreogram in the binding fraction of chromatographic separation - three zones with relative molecular weight 15 000 - 21 000. These zones correspond by their relative molecular weights to relative molecular weight of histones. The last zone was corresponding to ~ 34 000. All of these zones were sent for identification using MALDI-TOF analysis. The antimicrobial activity was detected in the non-binding fraction and in oviductal fluid. Contrary to this, the antimicrobial activity was not present in the bound fraction, due to the fact that in this case only an insignificant amount of proteins was found in the bound fraction. The presence of the glycosidases activity was shown in oviductal fluid. (In Czech)
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Isolation and characterisation of oviductal proteins involved in formation of sperm oviductal reservoir in cow
 Kračmerová, Jana ; Liberda, Jiří (advisor) ; Postlerová, Pavla (referee)
The oviduct plays an important role in the complex process of fertilization and in the early phase of embryo development. Isolation and characterization of some components of bovine oviduct participating in the formation of oviductal sperm reservoir was a subject of the present study. Two affinity sorbents containing immobilized components of bull seminal plasma (non-separated proteins of bull seminal plasma and their phosphorylcholine binding fraction) were prepared and used for the study of interaction of immobilized ligands with components of oviductal epithelium. Two types of preparation of oviductal membrane proteins of the isthmus region were isolated: complete membrane proteins and the apical fraction of membrane proteins. These preparations were separated using prepared affinity matrices. in both cases only an insignificant amount of proteins was found in the adsorbed fractions. Further attention has been paid to the identification of four protein zones detected after electrophoretic separation in the presence of SDS (rel. mol. wt. 15 500 - 18 500) in the fraction of apical membrane proteins. These proteins were identified after the electrophoretic separation and tryptic digestion using MS analysis as bovine histones H4, H2A typ 2-C, H2B typ 1-K a H3.3. Proteins identified as bovine...
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