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The use of molecular markers for the study of the impact of global change on fungal communities
Faltysová, Julie ; Baldrian, Petr (advisor) ; Koukol, Ondřej (referee)
Fungi are an essential part of life on Earth. Besides their main role in decomposition of organic matter, they belong among key symbiotic organisms and they take part in providing a range of ecosystem processes important for humankind such as primary production and with that related food availability or soil water regulation. For the study of fungal distribution, we often use a method called DNA metabarcoding which combines the use of specific molecular markers, most commonly universal fungal ITS, and high-throughput sequencing. The importance of the method has been rising in the current time of changing environment and it has helped us observe changes in fungal communities due to global change. It seems that primarily various climatic factors such as warming, changes in rainfall patterns and nitrogen deposition have strongly influenced community and fungal guilds composition. Consequently, these changes may potentially have an impact on ecosystem functioning so that their overall balance can be in threat. Key words: molecular markers, DNA metabarcoding, fungal community, warming, climate change, nitrogen deposition
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