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Identification of yeasts from interspecific varieties of grapes
Sadel, Peter ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
The main goal of my diploma thesis was to identify and characterize yeasts from must Hibernal and also collection yeasts by using methods called RFLP-PCR (Restriction Fragment Length Polymorphism - Polymerase Chain Reaction). Theory was the first part of my diploma thesis which dealt with wine, yeasts and molecular methods. Theory section was followed by experimental section divided into two parts. The main goal of the first part was to characterize and identify yeasts from must Hibernal by using PCR and RFLP-PCR methods. In the samples there were found yeasts Saccharomyces and Pichia. The second experimental part of my diploma thesis had a goal to extend the database of new yeasts using the same methods mentioned in the first part of experimental section.
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Factors influencing the quality of red wine
Zechmeisterová, Lucie ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.
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Basic features of wine fermented from freerun and pressed must
Becková, Eliška ; Šalplachta, Jan (referee) ; Vespalcová, Milena (advisor)
My work is engaging in winemaking of white and red wines. Theoretic processes and goings during winemaking is describe in the first part of this work. It deals harvest of kapes, fermetation, clearing wine, chemical content of grapes and wine, deseases and abnormality of wines. There are this described choosen variettes, their grapes were processed practically. The third part of this work is dealed in practice winemaking and chemical analyse sof wines. Musts from the start of pressing, from the first dressing and from the end dressing fermented to the end dressing . They were fermented and than analysed by chemical and tasting tests. The discussion about the dependence of the quality of wines on the kind of the must during pressing is presented in the end of this work. The results are motivated by chemical analyses and senzoric tasting of the experts.
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|
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Identification of yeasts from interspecific varieties of grapes
Sadel, Peter ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
The main goal of my diploma thesis was to identify and characterize yeasts from must Hibernal and also collection yeasts by using methods called RFLP-PCR (Restriction Fragment Length Polymorphism - Polymerase Chain Reaction). Theory was the first part of my diploma thesis which dealt with wine, yeasts and molecular methods. Theory section was followed by experimental section divided into two parts. The main goal of the first part was to characterize and identify yeasts from must Hibernal by using PCR and RFLP-PCR methods. In the samples there were found yeasts Saccharomyces and Pichia. The second experimental part of my diploma thesis had a goal to extend the database of new yeasts using the same methods mentioned in the first part of experimental section.
|
|
|
Factors influencing the quality of red wine
Zechmeisterová, Lucie ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.
|
|
|
Basic features of wine fermented from freerun and pressed must
Becková, Eliška ; Šalplachta, Jan (referee) ; Vespalcová, Milena (advisor)
My work is engaging in winemaking of white and red wines. Theoretic processes and goings during winemaking is describe in the first part of this work. It deals harvest of kapes, fermetation, clearing wine, chemical content of grapes and wine, deseases and abnormality of wines. There are this described choosen variettes, their grapes were processed practically. The third part of this work is dealed in practice winemaking and chemical analyse sof wines. Musts from the start of pressing, from the first dressing and from the end dressing fermented to the end dressing . They were fermented and than analysed by chemical and tasting tests. The discussion about the dependence of the quality of wines on the kind of the must during pressing is presented in the end of this work. The results are motivated by chemical analyses and senzoric tasting of the experts.
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