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Consequences of experimentaly induced perinatal cerebral ischemic lesion
Bahníková, Eva ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
1 Abstract Title: Consequences of experimentally induced perinatal cerebral ischemic lesion. Aim: This thesis aims to present the issues and analyze current knowledge in the field of perinatal brain damage, particularly perinatal cerebral ischemic lesions as the most common brain infarction in children. Basic characteristics of the disease, syndromology, pathophysiological mechanisms and risk factors are emphasized. Following the theoretical background the thesis analyzes current trends as well as the limitations of the diagnostics and therapy. The aim is to highlight the need for early diagnosis and emphasize the potential of preventive treatment strategies. The practical part follows the theoretical background and expands the topic on the analysis of motor and behavioral consequences of experimentally induced perinatal ischemic stroke. Method: Cerebral focal ischemia was induced experimentally by photothrombotic method to seven days old rats. Rose Bengal intravenously followed by continual illumination of the senzorimotor cortex using a green laser beam for 10 minutes was induced platelet aggregation and subsequent thrombosis. Eight animals at age of two months were evaluated for 7 days via observational cages PhenoTyper. Results: The theoretical part collected and processed theoretical data on the...
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Functional consequences of perinatal hypoxia-ischemia in rat
Nováková, Eva ; Otáhal, Jakub (advisor) ; Riljak, Vladimír (referee)
Title: Functional consequences of perinatal hypoxia-ischemia in rat Objectives: The aim of this diploma thesis is to design a set of behavioral tests which provide an effective assessment of motor and cognitive-behavioural deficits in adults rats after experimental hypoxic-ischemic insult during the perinatal period (P7). Supposed benefit is to establish a model of motor and cognitive-behavioural abilities of individuals after this procedure. Methods: The present thesis has a theoretical-empirical character. The practical part describes how the experiment was performed. 32 long Evans Rats were randomly devided into two groups: experimental group (HIE) and control group (Ctrl). The method to produce hypoxic-ischemic brain damage in the 7 day-old rats consisted of right common carotid ligation followed by systemic hypoxia by the inhalation of 8% oxygen and 92% nitrogen. The adult animals (55-75 days old) were tested by the following list of behavioral tests: Bar holding test, Rotarod test, Ladder rung walking test, Reaching test, Open field test and Morris water maze test. Sigma Plot and Microsoft Excel 2010 were the programs used for statistical analysis. Results: Results of Open field test, Ladder rung walking test and Morris water maze test confirmed that hypoxic-ischemic insult affects the...
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Evaluation of motoric deficit in children after posterior fossa brain tumor surgery
Jirousková, Jitka ; Medunová, Kateřina (advisor) ; Jevič, Filip (referee)
Objective: The main goal of this study was to collect and summarize all available data on both short term effects and late effects of posterior cranial fossa tumor treatment on children patients' motoric abilities and to find out any possible correlation between a decline in motoric abilities and the type of treatment they received. Following previous studies on the topic, this study set a goal to design a testing procedure for areas in which decline of motoric abilities manifests the strongest and to put this procedure to test in the practical part of the study. Methods: Based on a research into specialized literature, four main areas in which a decline of motoric abilities occurs in posterior cranial fossa tumor patients have been identified. Those are ataxia, balance, physical condition and functional activities. For Ataxia the SARA test was used, for physical condition the 6MWT test, for balance the PCTSIB test and for functional activities the BOT-2 test, respectively. Furthermore we observed the overall quality of life of selected patients through a PedsQL questionnaire. The group of patients selected for this experiment consisted of nine individuals who underwent posterior cranial fossa tumor treatment more than two years before. Results: The SARA (p=0,020) a 6MWT (p<0,0001) tests proved a...
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Consequences of experimentaly induced perinatal cerebral ischemic lesion
Bahníková, Eva ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
1 Abstract Title: Consequences of experimentally induced perinatal cerebral ischemic lesion. Aim: This thesis aims to present the issues and analyze current knowledge in the field of perinatal brain damage, particularly perinatal cerebral ischemic lesions as the most common brain infarction in children. Basic characteristics of the disease, syndromology, pathophysiological mechanisms and risk factors are emphasized. Following the theoretical background the thesis analyzes current trends as well as the limitations of the diagnostics and therapy. The aim is to highlight the need for early diagnosis and emphasize the potential of preventive treatment strategies. The practical part follows the theoretical background and expands the topic on the analysis of motor and behavioral consequences of experimentally induced perinatal ischemic stroke. Method: Cerebral focal ischemia was induced experimentally by photothrombotic method to seven days old rats. Rose Bengal intravenously followed by continual illumination of the senzorimotor cortex using a green laser beam for 10 minutes was induced platelet aggregation and subsequent thrombosis. Eight animals at age of two months were evaluated for 7 days via observational cages PhenoTyper. Results: The theoretical part collected and processed theoretical data on the...
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The effect of dynamic neuromusculur stabilization on motor deficit in patients after stroke
Zelenková, Jana ; Kobesová, Alena (advisor) ; Demeková, Júlia (referee)
The aim of the study was to evaluate the effect of specific rehabilitation concept on the motor deficit in patients after stroke. This concept is used at the Department of inpatient rehabilitation FN Motol, the main component is exercise on the principles of dynamic neuromuscular stabilization (DNS) and is based on the principles of developmental kinesiology. The study included 12 patients after stroke. Probands participated in the 3 week therapy, exercise was carried out twice a day. Significant (p <0.05) improvement in motor function of the arm, hand, leg and foot was demonstrated by using Student`s paired t-test. There was a statistically significant improvement in postural control and reducing shoulder pain (all assessed using a specific scale: Chedoke McMaster Stroke Assessment). Changes in the degree of spasticity were evaluated by Modified Ashworth scale. A statistically significant improvement in the degree of spasticity was observed in these muscle groups: the elbow flexors , adductors of lower limb, knee extensors and hamstrings. It has been shown that the concept of dynamic neuromuscular stabilization can be advantageously used in selected patients after stroke. It is appropriate to supplement the DNS therapy with training of ADL.
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