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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Kaštánek,, Petr (referee) ; Svěrák, Tomáš (advisor)
The bachelor's thesis is focused on cultivation of Chlorella microalgae species. Theoretical part deals with microalgae characteristics, their usage and methods of their cultivation. It is focused on the most important growth factors of chlorella and compares findings from earlier studies. Experimental part contains observations of microalgae growth in different environments and behavioural comparison of autotrophic and heterotrophic cultures. Next, the influence of temperature and light intensity on microalgae growth was observed, using different combinations of these factors. Experimental findings were evaluated from gained biomass concentration and absorbance determined by spectrophotometric method.
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Optimization of cultivation conditions and production of metabolites by microalgae of the genus Desmodesmus and Scenedesmus in photobioreactors.
Kubalová, Michaela ; Mikulíková, Renata (referee) ; Szotkowski, Martin (advisor)
Nowadays, a lot of attention is being paid to the biotechnology industry. Within this industry, are with the help of microorganisms produced various useful substances, such as chlorophyll and carotenoids, which are important antioxidant substances, but also fatty acids, sterols and ubiquinone. This thesis is focused on the cultivation of selected strains of microalgae of the genus Scenedesmus and Desmodesmus. In the first part of the thesis, the algae strains and studied metabolites are described. In the second, experimental part, different types of alcohol were applied to the selected strains as stress conditions, with the aim of increasing the production of intracellular metabolites. Further, fermentor cultivation was carried out, in which a combination of two different alcohols was applied. Subsequently, cultivation using a flat-panel photobioreactor was also carried out, in which light with different wavelengths was used. Several chromatographic techniques were used for the analysis and the results of the analyzes are presented in the experimental part.
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Issues of the algae Chlorella production in flow bioreactors
Jankovičová, Kristína ; Kaštánek,, Petr (referee) ; Svěrák, Tomáš (advisor)
The bachelor's thesis is focused on cultivation of Chlorella microalgae species. Theoretical part deals with microalgae characteristics, their usage and methods of their cultivation. It is focused on the most important growth factors of chlorella and compares findings from earlier studies. Experimental part contains observations of microalgae growth in different environments and behavioural comparison of autotrophic and heterotrophic cultures. Next, the influence of temperature and light intensity on microalgae growth was observed, using different combinations of these factors. Experimental findings were evaluated from gained biomass concentration and absorbance determined by spectrophotometric method.
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