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System for measurement of concentration of intracellular calcium in LabVIEW
Kormanovskaya, Elena ; Kolářová, Jana (referee) ; Čmiel, Vratislav (advisor)
The master’s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis included theoretical and practical parts. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. Literary solution is presented. Block diagram of system for measurement of concentration of intracellular calcium at a graphical programming environment LabVIEW is presented in practical part of master’s thesis.
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Segmentation in microscopic images
Vlk, Jaroslav ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
Work studying the properties of microscopic images and then the following applies in the segmentation of the image. Work is trying to use a simpler methods image processing mainly. At the same time, deals with methods for preprocessing image. In the choice of methods and the use of emphasis on speed and simplicity of calculation.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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Segmentation in microscopic images
Vlk, Jaroslav ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
Work studying the properties of microscopic images and then the following applies in the segmentation of the image. Work is trying to use a simpler methods image processing mainly. At the same time, deals with methods for preprocessing image. In the choice of methods and the use of emphasis on speed and simplicity of calculation.
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System for measurement of concentration of intracellular calcium in LabVIEW
Pochlopeň, Timotej ; Ronzhina, Marina (referee) ; Čmiel, Vratislav (advisor)
Master‘s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. The theory dealing with confocal microscopy is presented and two techniques are compared. Literary solution is presented. The practical part describes the program with a graphical user interface used for acquisition and analysis of data measuring dynamics of intracellular calcium concentration. The experimental part describes the preparation and measurement of cells and evaluation of experiments.
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System for measurement of concentration of intracellular calcium in LabVIEW
Kormanovskaya, Elena ; Kolářová, Jana (referee) ; Čmiel, Vratislav (advisor)
The master’s thesis deals with the methods and system for measurement of concentration of intracellular calcium in LabVIEW. The thesis included theoretical and practical parts. The thesis covers the theory of fluorescence principles, fluorescence methods and indicators for measurement of concentration of intracellular calcium. The procedure of measurement by means of fluorescence microscope, camera and indicators is also mentioned. Literary solution is presented. Block diagram of system for measurement of concentration of intracellular calcium at a graphical programming environment LabVIEW is presented in practical part of master’s thesis.
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