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Influence of clinical nutrition in elderly patients
Jančarová, Kateřina ; Matejovičová,, Milena (referee) ; Němcová, Andrea (advisor)
This master’s thesis deal with the influence of supplementation with omega-3 fatty acids on cognitive functions and on the blood marker of clients in the retirement home. The main target of this thesis is demonstrate the improvement of cognitive functions and lipid and glycemic profile after supplementation with the dietary supplement. Suplementation was serve to the clients for the period of 3 months 3 times a day in the amount of 5 ml per dose. In the teoretical part of the thesis was explained the process of aging and the problematics of omega-3 fatty acids. The practical part evaluates the three-month supplementation with a dietary supplement and its mainly focus to cognitive tests MMSE and blood test results. The thesis is deal with effect of the product on the blood marker, mainly the lipid profile and glycemic profile were determined. Laboratory analysis was used to determine lipid numbers. Fatty acids analysis was performed by gas chromatography and vitamins analysis was performed by liquid chromatography. Based on recent findings, it was assumed that supplementation of omega-3 fatty acids can improve the cognitive functions in older adults as well as their lipid and glycemic profile. The improvements have been confirmed in cognitive functions but not in lipid profile. This facts may have been influenced by several factors, such as inappropriate composition of the menu, the consumption of foods with a high content of saturated fatty acids outside the menu, the administration of medication or overall short supplementation time period.
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The effect of a low carbohydrate diet on the compensation of type 2 diabetes mellitus
Sedlářová, Lenka ; Sucharda, Petr (advisor) ; Sadílková, Aneta (referee)
Type 2 diabetes mellitus (T2D) is a chronic metabolic disease characterized by insulin resistance and several secondary complications. The prevalence of patients is constantly increasing, and thus the effort to improve the quality of therapy, new findings, and procedures capable of being applied in clinical practice are sought. Patients live with the disease for a long time, and the purpose of treatment is to improve the quality of life of diabetics and delay the onset of associated health complications. A combination of regimen measures is essential for successful treatment. Dietary measures in the form of a low-carbohydrate diet are still a popular choice of dietary options among diabetics. The aim of the practical part of the work was to prove the positive effect of a low- carbohydrate diet on T2D compensation. The primary objective was to demonstrate a significant reduction in glycated hemoglobin (HbA1c) in patients who eat or have eaten according to the principles of a low-carbohydrate diet for at least 12 months. Secondary objectives were set as follows: reduction of relative weight (BMI), reduction of pharmacotherapy and/or reduction of daily doses of antidiabetic drugs, reduction of fasting blood glucose and improvement of the lipid spectrum. The research group included patients from the...
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Qualitative analysis of selected glycosphingolipids subclasses isolated from human blood plasma using HILIC-ESI-MS/MS
ŠIKLOVÁ, Michaela
This bachelor thesis deals with the qualitative analysis of selected subclasses of glycosphingolipids isolated from human blood plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). Glycosphingolipids are ubiquitous and structurally very diverse components of cell membranes involved in many cellular functions. It has been shown that changes in the structures of glycosphingolipids and their distinct expression can pose a key factor in the development of numerous diseases and different types of cancer.The theoretical part of this thesis is dedicated to a summary of fundamental knowledge about glycosphingolipids, their biosynthesis, classification, biological functions and their association with various types of diseases and cancers. Furthermore, methods of isolation, purification and analytical possibilities for the determination of glycosphingolipids in biological samples are described here.In the experimental part, blood samples were taken and processed together with the preparation of the necessary solutions, plasma samples, and standards. Moreover, a test analysis, optimization of mobile phase gradient and comparison of the most frequently used extraction methods according to Folch, Bligh and Dyer, Matyash, and Alshehry alongside fractionation of lipid extract is described in this part. An integral part of the experimental section was the identification and structural characterization of glycosphingolipids within selected subclasses, including the generation of GSL profiles in human blood plasma. In conclusion, the results of the work and answers to the stated research questions are summarized, including the evaluation of possible improvements in this field.
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Influence of clinical nutrition in elderly patients
Jančarová, Kateřina ; Matejovičová,, Milena (referee) ; Němcová, Andrea (advisor)
This master’s thesis deal with the influence of supplementation with omega-3 fatty acids on cognitive functions and on the blood marker of clients in the retirement home. The main target of this thesis is demonstrate the improvement of cognitive functions and lipid and glycemic profile after supplementation with the dietary supplement. Suplementation was serve to the clients for the period of 3 months 3 times a day in the amount of 5 ml per dose. In the teoretical part of the thesis was explained the process of aging and the problematics of omega-3 fatty acids. The practical part evaluates the three-month supplementation with a dietary supplement and its mainly focus to cognitive tests MMSE and blood test results. The thesis is deal with effect of the product on the blood marker, mainly the lipid profile and glycemic profile were determined. Laboratory analysis was used to determine lipid numbers. Fatty acids analysis was performed by gas chromatography and vitamins analysis was performed by liquid chromatography. Based on recent findings, it was assumed that supplementation of omega-3 fatty acids can improve the cognitive functions in older adults as well as their lipid and glycemic profile. The improvements have been confirmed in cognitive functions but not in lipid profile. This facts may have been influenced by several factors, such as inappropriate composition of the menu, the consumption of foods with a high content of saturated fatty acids outside the menu, the administration of medication or overall short supplementation time period.
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