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The role of lactate shuttle in ischemic-reperfusion injury of rat heart adapted to chronic hypoxia
Kolář, David ; Žurmanová, Jitka (advisor) ; Nováková, Olga (referee)
Adaptation to hypoxia is a well-known phenomenon increasing myocardial resistance to ischemia-reperfusion (I/R) injury as an appropriate physical exercise which improves the contractile function of the heart. Lactate is a major energy substrate for the heart muscle during physical activity and hypoxia. The metabolism of lactate was and still is associated with muscle fatigue, but in the last decades it has been considered its significant modulating function of metabolism during exercise at cellular level and whole organism level. It has been shown that its effects might be similar to the effects of hypoxia and its oxidized form, pyruvate, has the cardioprotective effects. The aim of this study was to compare the expression of LDHA and LDHB isoforms between left and right ventricle in the cardioprotective scheme of adaptation to hypoxia. Another objective/goal was to determine the left ventricular response to I/R insult in the perfused heart model adapted to hypoxia compared with the normoxic controls on/at the expression level of both LDH isoforms. Our results showed differences in the LDHA expression in the left and right ventricle and an increased response of the left ventricle to I/R insult in rats adapted to hypoxia which is reflected at the expression level of both isoforms. Key words: heart,...
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Metabolism adaptation to excercise
Nencini, Ricky ; Šulc, Miroslav (advisor) ; Stiborová, Marie (referee)
Skeletal muscles are remodeled in response to chronic exercise training. Training induces adaptations, which can be reflected by changes in contractile protein functi- ons, mitochondrial amount and functions and also in specific enzyme activity. Due to extensive training muscle proteins and enzymes permeate through sarcoplasm and can be detected at blood plasma. Six male rowers, 19,6 ˘ 2,1 years old (trained), six sedentary men, 20,2 ˘ 1,8 years old (untrained) were included within this report. Except mentioned groups, two individual clinical cases: one male user of androsten, 28 years old, and one male user of erythropoetin (EPO), 32 years old, were also included at this theses. To report any species influence, the groups (five females per group) of two domestic animal species (cow, pig) with different treatment (housed, outdoor) were also included. Blood concentration of erythrocytes, blood plasma concentration of proteins, acti- vity of lactatedehydrogenase (LDH), malatedehydrogenase (MDH) and enoyl-CoA- hydratase were measured as well as quantitative pattern of LDH izoenzymes. The only one difference for man was observed: a significantly higher concentration of plasma protein for untrained group. On the other hand, the outdoors cows had a significantly higher concentration of erythrocytes and activity...
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The role of lactate shuttle in ischemic-reperfusion injury of rat heart adapted to chronic hypoxia
Kolář, David ; Žurmanová, Jitka (advisor) ; Nováková, Olga (referee)
Adaptation to hypoxia is a well-known phenomenon increasing myocardial resistance to ischemia-reperfusion (I/R) injury as an appropriate physical exercise which improves the contractile function of the heart. Lactate is a major energy substrate for the heart muscle during physical activity and hypoxia. The metabolism of lactate was and still is associated with muscle fatigue, but in the last decades it has been considered its significant modulating function of metabolism during exercise at cellular level and whole organism level. It has been shown that its effects might be similar to the effects of hypoxia and its oxidized form, pyruvate, has the cardioprotective effects. The aim of this study was to compare the expression of LDHA and LDHB isoforms between left and right ventricle in the cardioprotective scheme of adaptation to hypoxia. Another objective/goal was to determine the left ventricular response to I/R insult in the perfused heart model adapted to hypoxia compared with the normoxic controls on/at the expression level of both LDH isoforms. Our results showed differences in the LDHA expression in the left and right ventricle and an increased response of the left ventricle to I/R insult in rats adapted to hypoxia which is reflected at the expression level of both isoforms. Key words: heart,...
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