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Kvantitativní zastoupení bakterií rodu Lactobacillus v lidském zažívacím traktu v závislosti na změně stravovacích návyků
Kolísková, Kateřina
This diploma thesis deals with the issue of quantitative representation of the bacterial family in the human digestion system depending on changes in eating habits. The start of literary digest addresses general characteristics of digestion microbiome and its bacterial represen-tation. The most comprehensive part belongs to individual factors, who influenced overall number of bacterial species inhabiting the digestion microbiome. The last chapters of the theoretical part belong to cultivative and molecular biological methods, which are used in examination of the human digestion tract. Experimental part focuses on differences in the number of individual bacteria in the samples of human excrements, whose presence was determined by cultivative methods. Isolation of bacterial DNA was performed, which was subsequently confirmed by polymerase chain reaction. In resulting values, we observed small changes (p < 0,05) in bacterial species L. acidophilus a L. rhamnosus at the end of the second period. All bacterial families, except Bacteroides, had downward tendency (p > 0,05). Based on acquired data from food records, we observed changes in consumption of basic macronutrients (protein, fat, carbohydrates) and fiber. Only in carbohydrates and sim-ple sugars, we observed statistically provable differences (p > 0,05). Final analysis proved that changes in eating habits did not influence the total number of bacterial species inhabiting digestion microbiome.
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Staphylococcus aureus - jeho výskyt a možnosti detekce v mléce a mléčných výrobcích
Fenclová, Denisa
The theoretical part of diploma thesis is focused on summarizing current knowledge about S. aureus, its pathogenesis, occurrence, and possibilities of its detection in milk and dairy products. The experimental part of diploma thesis is aimed at determining S. aureus using various cultivation methods. S. aureus was determined using B-P agar with the addition of RPF, ClearMilku test, MicroFast®, and PetrifilmTM. The determination was evaluated as follows: B-P agar with the addition of RPF > PetrifilmTM > MicroFast® > ClearMilk test. Another part of the experimental diploma thesis deals with the possibility of detecting S. aureus using a newly developed biosensor detection system.
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Characterization of fungal community in spruce (Picea abies) litter using cultivation and T-RFLP
Kolářová, Zuzana ; Koukol, Ondřej (advisor) ; Brabcová, Vendula (referee)
Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....
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Characterization of fungal community in spruce (Picea abies) litter using cultivation and T-RFLP
Kolářová, Zuzana ; Koukol, Ondřej (advisor) ; Brabcová, Vendula (referee)
Fungi have a key role in the decomposition of coniferous litter and affect nutrient cycling in forest ecosystems. Therefore, great emphasis is placed on exploring the diversity of these organisms. The aim of this thesis was to describe fungal diversity in spruce litter and revealed temporal development of this community in a forest regenerating after bark beetle outbreak. Another objective was to compare sites with different length of bark beetle damage. The study area was located in the Bohemian Forest mountain range. Litter bags with spruce needles were placed on the forest soil and several samplings were performed in the course of three years. Diversity of fungi and changes in the fungal community were assessed by two methods: cultivation of needles on 2ř MEA and fingerprinting method T-RFLP. In total 71 fungal species were obtained from needles during a three-year succession by cultivation approach. Using T-RFLP 122 different fragments were generally recorded. The dominant species were Scleroconidioma sphagnicola, Thysanophora penicillioides, Hormonema dematiodes, Ceuthospora pinastri, species of genus Chalara, Trichoderma polysporum, Mycena galopus and unknown species Helotiales sp. 1. Primary saprotrophs occured in the community mainly in first 8 months and then were replaced by basidiomycetes....
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Determination and diagnostics of beta-hemolytics streptococcus in clinical microbiology
VOŘÍŠKOVÁ, Martina
The genus of Streptococcus includes gram-positive catalase of negative coccus, which rank into pairs and chains and their colonies are also sometimes very small in enriched soils. Most of species are selectively anaerobic. This genus includes a large number of species with different significance for people ´s disorders, and also the species significant for veterinary medicine. Beta-hemolytic or pyogenic Streptococci important in human medicine include first of all streptococci of A and B group. Streptococcus pyogenes (A group) is the cause of either localised purulent infections, or invasive and toxic diseases, and also late consequences of these infections. The carrying of beta-hemolytic streptococci of B group (Streptococcus agalactiae) is significant especially in pregnant women because it can threaten the mother and the newborn as well. They are the most important causers of newborn meningitis and sepsis. The aim of the thesis is to find out information of determination and detection methods concerning beta-hemolytic streptococci. It is necessary to devote our attention to each streptococcus and determine its type by cultivation, eventually by further supplementary methods. The main role in diagnostics is played by classical cultivation in common (blood agar) or special enriched soils. Each microbiological laboratory chooses its own laboratory procedures. The use of cultivation for capture of beta-hemolytic streptococci and determination of their types by PYR test (for the proof of A group streptococci) and latex agglutination (for confirmation of other groups) seem to be the most convenient. CAMP-test is still the most frequently used method for the proof of group B streptococci. For exclusion of later consequences the assessment of anti-streptolysin O (ASLO) or antideoxyribonuclasis B (ADNB) in serum are often used. All beta-hemolytic streptococci must be regarded as important pathogens namely in whatever locality, that is why the knowledge of basic and special laboratory methods which lead to identification of particular types of these streptococci is very important for clinical microbiology and the treatment of patients.
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