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Nordic walking - gait analysis of older adults.
Novotný, Petr ; Vilímek, Miloslav (advisor) ; Horák, Zdeněk (referee)
Title: Nordic walking - analysis of senior walking Aims: This thesis aims to make a kinematic and kinetic analysis of gait and NW in elderly using Qualisys and Kistler. Afterwards, it also aims to compare the selected parameters with each other and, based on the results, to objectify the effect of NW on the gait of the elderly. Methods: The theoretical part of this thesis was processed using books and electronic sources obtained from online databases (PubMed, Medline, and Google Scholar). The research part was done in the form of an experiment. Fifteen women aged between 65 and 83 years (mean age 74) who regularly practiced Nordic walking under professional guidance participated in the research. These women were measured while walking and NW. A 3D kinematic analysis of walking and NW was performed using the Qualisys Motion Capture camera system. Ground reaction forces were measured using Kistler force plates placed in the captured kinematic analysis path. Data from these instruments was processed in Qualisys Track Manager and Microsoft Office Excel. Selected gait parameters that were analyzed included stride length, double-step length, gait speed, stride cadence, standing phase duration, lateral pelvic deviation, hip flexion and extension, knee joint flexion, dorsal and plantar flexion of the ankle...
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Kinetic analysis of signals from gait laboratory.
Benešovská, Veronika ; Mézl, Martin (referee) ; Svozilová, Veronika (advisor)
Gait analysis has an irreplaceable meaning in many various fields. Thanks to the Vicon system, which is used in gait laboratory, the movement of individual body parts can be captured. The purpose of this work is to process data records from gait laboratory, and kinetic analysis in Matlab program. The main target of the bachelor thesis is to compare detected values, which were evaluated by kinetic analysis of the data obtained from tested subject during performing the exercise and creating GUI.
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Multidisciplinary characterization of interactions between oestrogen hormones and their receptors during sperm capacitation.
Bosáková, Tereza
This doctoral thesis was focused on the multidisciplinary characterizatrion of interactions between hormones and their receptors during sperm capacitation, which is the final step in sperm maturation in mammals and the key event to achieve egg fertilization. Three oestrogenic hormones (17β-estradiol (E2), 17α-estradiol (αE2), 17α-ethynylestradiol (EE2)), which are part of environmental pollution, were selected for the study. These hormones are called endocrine disruptors as they can cause disorders of the hormonal system in animals, thereby adversely affecting physiological processes, including reproduction. A new analytical HPLC-MS/MS method was developed for monitoring of concentration changes of oestrogens (E2, αE2 and EE2) during time-dependent capacitation of mouse and rat sperm in vitro. For individual oestrogens, the concentration dependences of free, unbound hormone on the capacitation time were measured for three concentrations of individual hormones (200, 20 and 2 μg/L). The obtained concentration data were converted into values of relative concentrations, which were subjected to kinetic analysis. Kinetic models were proposed for the interaction of individual hormones with sperm, which were described by a system of differential equations with optimization of rate constants and molar...
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Analysis of biologically active compounds using high performance separation methods
Adamusová, Hana ; Bosáková, Zuzana (advisor) ; Jelínek, Ivan (referee) ; Tůma, Petr (referee)
In the first part of this doctoral thesis, a new analytical HPLC-MS/MS method for monitoring of concentration changes of 17β-estradiol (βE2) during in vitro mouse sperm capacitation was developed. Capacitation was performed for three initial concentrations of βE2 (200, 20 and 2 μg/L). For all the concentrations a similar trend for the total unbound βE2 was observed. In general, the βE2 concentration decreased to reach its minimum and then increased again. The position of the minimum differed for the individual tested βE2 concentrations. Experimentally obtained results were subjected to the kinetic analysis. The curves fitted through the experimentally determined points displayed an autocatalytic character. For the agreement between the curves obtained by fitting through the experimental points and the theoretical calculated curves, it is necessary to assume that the first step is adsorption of βE2 onto the surface of the sperm controlled by Langmuir isotherm. The kinetic study was also used to study the effects of fluorides and aluminium fluoride complexes on the capacitation of mouse sperm. The experimental points were in very good agreement with the shape of the theoretical curves and this fact verifies the mechanism of the mouse sperm capacitation kinetics. In the second part of this work, two...
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Multidisciplinary characterization of interactions between oestrogen hormones and their receptors during sperm capacitation.
Bosáková, Tereza ; Komrsková, Kateřina (advisor) ; Smrček, Stanislav (referee) ; Postlerová, Pavla (referee)
This doctoral thesis was focused on the multidisciplinary characterizatrion of interactions between hormones and their receptors during sperm capacitation, which is the final step in sperm maturation in mammals and the key event to achieve egg fertilization. Three oestrogenic hormones (17β-estradiol (E2), 17α-estradiol (αE2), 17α-ethynylestradiol (EE2)), which are part of environmental pollution, were selected for the study. These hormones are called endocrine disruptors as they can cause disorders of the hormonal system in animals, thereby adversely affecting physiological processes, including reproduction. A new analytical HPLC-MS/MS method was developed for monitoring of concentration changes of oestrogens (E2, αE2 and EE2) during time-dependent capacitation of mouse and rat sperm in vitro. For individual oestrogens, the concentration dependences of free, unbound hormone on the capacitation time were measured for three concentrations of individual hormones (200, 20 and 2 μg/L). The obtained concentration data were converted into values of relative concentrations, which were subjected to kinetic analysis. Kinetic models were proposed for the interaction of individual hormones with sperm, which were described by a system of differential equations with optimization of rate constants and molar...
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Kinetic analysis of signals from gait laboratory.
Benešovská, Veronika ; Mézl, Martin (referee) ; Svozilová, Veronika (advisor)
Gait analysis has an irreplaceable meaning in many various fields. Thanks to the Vicon system, which is used in gait laboratory, the movement of individual body parts can be captured. The purpose of this work is to process data records from gait laboratory, and kinetic analysis in Matlab program. The main target of the bachelor thesis is to compare detected values, which were evaluated by kinetic analysis of the data obtained from tested subject during performing the exercise and creating GUI.
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Analysis of biologically active compounds using high performance separation methods
Adamusová, Hana ; Bosáková, Zuzana (advisor) ; Jelínek, Ivan (referee) ; Tůma, Petr (referee)
In the first part of this doctoral thesis, a new analytical HPLC-MS/MS method for monitoring of concentration changes of 17β-estradiol (βE2) during in vitro mouse sperm capacitation was developed. Capacitation was performed for three initial concentrations of βE2 (200, 20 and 2 μg/L). For all the concentrations a similar trend for the total unbound βE2 was observed. In general, the βE2 concentration decreased to reach its minimum and then increased again. The position of the minimum differed for the individual tested βE2 concentrations. Experimentally obtained results were subjected to the kinetic analysis. The curves fitted through the experimentally determined points displayed an autocatalytic character. For the agreement between the curves obtained by fitting through the experimental points and the theoretical calculated curves, it is necessary to assume that the first step is adsorption of βE2 onto the surface of the sperm controlled by Langmuir isotherm. The kinetic study was also used to study the effects of fluorides and aluminium fluoride complexes on the capacitation of mouse sperm. The experimental points were in very good agreement with the shape of the theoretical curves and this fact verifies the mechanism of the mouse sperm capacitation kinetics. In the second part of this work, two...
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