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Production of toxins by Bacillus subtilis and their roles in interspecies competitions.
Šureková, Kristína ; Krásný, Libor (advisor) ; Mikušová, Gabriela (referee)
Bacillus subtilis is a gram positive soil bacterium that is surrounded by many other microorganisms its environment. That is why it is necessary for the bacterium to be able to fight with these microorganisms for the nutrients and living space. B. subtilis contains the modules in its genetic make-up that improve its ability to compete. These modules are called the toxin-antitoxin systems. This Diploma Thesis is trying to identify yet undescribed extracellular toxins produced by the wild type BSB1 strain of B. subtilis. The related microorganism Bacillus megaterium was used as a competing bacterium. The contact-dependent or independent manner of killing the competing bacterium was demonstrated using this model. By deletion analysis and comparisons of the genomes of the various strains of B. subtilis, the SPβ prophage was first identified as a region containing an unknown toxin(s). Analysis of the extracellular proteome of B. subtilis subsequently revealed an unknown toxin (or toxin complex, respectively) of the molecular weight exceeding 100 kDa. Even more fascinating was the finding that such a large protein molecule is resistant to the pancreatic protease, trypsin. Subsequent non-enzymatic cyanogen bromide cleavage of the extracellular proteins and their analysis by mass spectrometry revealed...
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Hodnocení toxicity průsakových vod ze skládky odpadů Kuchyňky
Studeníková, Petra
Thesis on Evaluation of the toxicity of the water seepage from landfill Kuchyňky is engaged in legislation, waste management, statistical data in the beginning. Then there is a theoretical description of the process of landfill, division of landfills and characteristics of the basic methods of ecotoxicology. The next part is devoted to informations about landfill Zdounky Kuchyňky, its location, natural conditions and monitoring data on the landfill. Then the chapter is devoted to the description of the test of growth of duckweed (Lemna minor L.) on water seepage from landfill Kuchyňky. In the last part of the thesis there is the evaluation of the results of a toxicity test of water seepage and rainwater from landfill Kuchyňky.
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