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Perinatal hypoxia: mechanisms and etiology
Laštůvka, Zdeněk ; Riljak, Vladimír (advisor) ; Cendelín, Jan (referee) ; Vízek, Martin (referee)
Perinatal asphyxia is a condition resulting from hypoxic-ischemic injury in utero or during birth and causing perinatal morbidity and mortality. Up until now, numerous models of neonatal hypoxia-ischemia were created and presented simulating brain damage patterns and motor deficits. Rice-Vannucci model is a leading representative of animal rodent models of hypoxic injury. It uses unilateral ligation of the common carotid artery in combination with exposure to hypoxic environment. Seven-day-old mouse pups underwent unilateral ligation of the common carotid artery in combination with exposure to 8 % oxygen hypoxic air (Rice-Vannucci model modification). Experimental groups with isolated carotid artery ligation or only exposure to hypoxia were created, too. To assess the spontaneous behavior changes of mice we used an automated system for the continuous tracking of small rodent behavior (LABORAS ™). On postnatal day 60, the spontaneous behavior of mice was analyzed in various behavioral domains. The observed areas for histological analysis were the cerebral cortex, CA1 and CA3 regions of the hippocampus, hilus, and the dorsal and ventral blades of the dentate gyrus. Induced hypoxia and ischemia together generated substantial behavioral and morphological changes which led to significant differences in...
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Motor and cognitive consequences of hypoxic-ischemic encephalopathy
Pernicová, Alice ; Otáhal, Jakub (advisor) ; Riljak, Vladimír (referee)
Author: Bc. Alice Pernicová Title: Motor and cognitive consequences of hypoxic-ischemic damage Objectives: The aim of this diploma work was asses the impact of Enriched Environment on development of rats after hypoxic-ischemic damage. Methods: Experiment was performed in 30 Long Evans rats from breeding in Institute of Physiology, Academy of Sciences of the Czech Republic. All rats were operated for dissection of carotid. Than randomly were half of rats chosen for hypoxic-ischemic damage. Group with hypoxic-ischemic damage were divided into control and experimental group, same procedure was with rats without HIE. For one week were rats from experimental group in special Enriched Environment cage, than they were split into smaller cages with Enriched Environment. Rats from control groups were in normal cages without special equipment. After defined time were all rats tested by chosen test: Reaching test, Ladder rung walking test, Bar holding test, Rotarod test, Morris water maze test, Open filed test. For data analysis were used Microsoft Excel 365, Sigma plot. Results: Results of test showed, that Enriched Environment can positively enhance motoric and cognitive deficit in rat development with hypoxic-ischemic damage. But it is obvious, that Enriched Environment can positively enhance development...
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Functional consequences of perinatal hypoxia-ischemia in rat
Nováková, Eva ; Otáhal, Jakub (advisor) ; Riljak, Vladimír (referee)
Title: Functional consequences of perinatal hypoxia-ischemia in rat Objectives: The aim of this diploma thesis is to design a set of behavioral tests which provide an effective assessment of motor and cognitive-behavioural deficits in adults rats after experimental hypoxic-ischemic insult during the perinatal period (P7). Supposed benefit is to establish a model of motor and cognitive-behavioural abilities of individuals after this procedure. Methods: The present thesis has a theoretical-empirical character. The practical part describes how the experiment was performed. 32 long Evans Rats were randomly devided into two groups: experimental group (HIE) and control group (Ctrl). The method to produce hypoxic-ischemic brain damage in the 7 day-old rats consisted of right common carotid ligation followed by systemic hypoxia by the inhalation of 8% oxygen and 92% nitrogen. The adult animals (55-75 days old) were tested by the following list of behavioral tests: Bar holding test, Rotarod test, Ladder rung walking test, Reaching test, Open field test and Morris water maze test. Sigma Plot and Microsoft Excel 2010 were the programs used for statistical analysis. Results: Results of Open field test, Ladder rung walking test and Morris water maze test confirmed that hypoxic-ischemic insult affects the...
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