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Interaction of modified polymers with liposomes
Dimidova, Aleksandra ; Kalina, Michal (referee) ; Mravec, Filip (advisor)
The aim of this work was to investigate the interactions of modified polymers with vesicular systems, focusing on hydrophobically modified biopolymers. The work included a literature search, design of experiments to study these interactions, execution of the experiments and evaluation of the results in terms of potential medical applications. Different methods of preparing liposomes were studied in order to optimize their physicochemical properties for efficient drug delivery. Although the preparation of liposomes from DPPC faced problems due to the quality of chemicals, the ethanol addition method resulted in the formation of stable liposomes. These liposomes showed good size and stability, and their interaction with sodium palmitoyl hyaluronate indicated the potential for increasing the size of liposomes and affecting their stability.
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Nanocarriers based on hydrophobized biopolymer
Velcer, Tomáš ; Hnyluchová, Zuzana (referee) ; Venerová, Tereza (advisor)
This work studies the properties of hydrophobically modified biopolymers for potential use in targeted drug delivery. Two samples of hydrophobically modified hyaluronan were selected. The critical micelle concentration (CMC) of these samples was measured using fluorescence spectroscopy, wherein it was found that micelle formation occurs over a wide concentration region. Environment of 0,15 M NaCl causes the decrease of CMC only slightly or not at all. Using the method of dynamic light scattering, particle size was measured. One of the samples exhibited a double size distribution. DLS results are consistent with the results of fluorescence spectroscopy at issue in aggregates forming near the CMC point. Zeta potential measurements provide information about stability of the samples. The results of this study confirm that one of the studied samples seems to be a suitable candidate for the post of drug carrier for targeted delivery.
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Colloidal particles marked with biopolymer
Pihíková, Dominika ; Víteček,, Jan (referee) ; Mravec, Filip (advisor)
The effect of hydrophobically modified hyaluronan on surfactants aggregation has been studied in this master’s thesis. The value of critical micelle concentration of anionic surfactant SDBS (sodium dodecylbenzensulfonate), cationic surfactant CTAB (cetyltrimethylamonnium bromide) and nonionic surfactant Triton X-100 (octylphenol ethoxylate) was determined by fluorescence spectroscopy using pyrene probe. Aggregation behavior of surfactants was performed with addition of hydrophobically modified hyaluronan of two molecular weights (17 kDa, 206 kDa) in aqueous solution. The greatest influence of hydrophobized hyaluronan on aggregation behavior was observed in system with cationic surfactant CTAB. Stability of system containing cationic surfactant and hydrophobically modified hyaluronan was established through zeta potential. Last part of thesis deals with size determination using dynamic light scattering.
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Colloidal particles marked with biopolymer
Pihíková, Dominika ; Víteček,, Jan (referee) ; Mravec, Filip (advisor)
The effect of hydrophobically modified hyaluronan on surfactants aggregation has been studied in this master’s thesis. The value of critical micelle concentration of anionic surfactant SDBS (sodium dodecylbenzensulfonate), cationic surfactant CTAB (cetyltrimethylamonnium bromide) and nonionic surfactant Triton X-100 (octylphenol ethoxylate) was determined by fluorescence spectroscopy using pyrene probe. Aggregation behavior of surfactants was performed with addition of hydrophobically modified hyaluronan of two molecular weights (17 kDa, 206 kDa) in aqueous solution. The greatest influence of hydrophobized hyaluronan on aggregation behavior was observed in system with cationic surfactant CTAB. Stability of system containing cationic surfactant and hydrophobically modified hyaluronan was established through zeta potential. Last part of thesis deals with size determination using dynamic light scattering.
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Nanocarriers based on hydrophobized biopolymer
Velcer, Tomáš ; Hnyluchová, Zuzana (referee) ; Venerová, Tereza (advisor)
This work studies the properties of hydrophobically modified biopolymers for potential use in targeted drug delivery. Two samples of hydrophobically modified hyaluronan were selected. The critical micelle concentration (CMC) of these samples was measured using fluorescence spectroscopy, wherein it was found that micelle formation occurs over a wide concentration region. Environment of 0,15 M NaCl causes the decrease of CMC only slightly or not at all. Using the method of dynamic light scattering, particle size was measured. One of the samples exhibited a double size distribution. DLS results are consistent with the results of fluorescence spectroscopy at issue in aggregates forming near the CMC point. Zeta potential measurements provide information about stability of the samples. The results of this study confirm that one of the studied samples seems to be a suitable candidate for the post of drug carrier for targeted delivery.
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