|
|
Determination of TauroLock stability
Matejčíková, Eva ; Mravcová, Ludmila (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with the study of the anticoagulant and antimicrobial agent TauroLockTM, which is manufactured by the german company TauroPharm GmbH. Based on the background research and taking into account the equipment of analytical laboratories of the Institute of Chemistry and Technology of Environmental Protection, optimal methods of analysis were proposed and a series of experiments were carried out which focused on the determination of the active substance and degradation products of taurolidine in TauroLockTM which was stored for a certain period of time in real conditions. The main task of this work was to determine the presence of taurolidine as the active substance and formaldehyde as the undesirable product of its decomposition.
|
|
|
Computer Simulation of Quadrupole Mass Filter
Kandra, Mário ; Kolářová, Jana (referee) ; Hejč, Jakub (advisor)
At our thesis, we deal with problematics of mass spectrometry. First, we are trying to explain basic physical principles, which are used by quadrupole filter or are appearing in concomitant phenomenons. In following section we will describe theoretical nature of quadrupole mass filter, stabilization and optimalization of device. Main focus of this thesis is dedicated to simulation of quadrupole mass filter and influence of parameters on trajectory of ion movement.
|
|
|
Adaptation of enzymatic assays for high-throughput screening with use of ECHO-MS
Kráľová, Zuzana ; Mertlíková Kaiserová, Helena (advisor) ; Šácha, Pavel (referee)
High throughput screening for potential inhibitors of enzyme purine nucleoside phosphorylase (PNP) was developed during the work on this thesis. Products of PNP-catalysed reaction, hypoxanthine and ribose-1-phosphate, were quantified using mass spectrometry. Advantages and disadvantages of an Echo® MS device were explored and compared to the previously used radioenzymatic method. The emphasis was put on assay robustness and automatization of the protocol. Acoustic droplet ejection technique (ADE) and contactless dispensing of chemicals were employed, using Echo® 550 and Certus Flex® devices. These allowed to reduce reaction volume from original 20 µl to 5 µl and perform the whole reaction in a 384-well plate, decreasing the costs and increasing the throughput. Using a developed Echo® MS method, the time of analysis was shortened from 8 minutes per sample for radiometric analysis to 0,2 minute per sample. Furthermore, enzyme assay parameters, such as PNP concentration, composition of reaction mixture, incubation time and reaction termination were optimized, following the rules of HTS. The method developed in this work was employed for PNP inhibitors screening and their IC50 determination. Moreover, the HTS method enabled screening of the whole library of IOCB in-house synthesized compounds (~ 8000)....
|
|
|
Analysis of Nicotine or Opium Alkaloids in Historical Pharmaceutical Relics from 18th Century
Belianský, Michal ; Nesměrák, Karel (advisor) ; Kubíčková, Anna (referee)
Belianský M.: Analysis of Nicotine or Opium Alkaloids in Historical Pharmaceutical Relics from 18th Century. Bachelor Thesis. Prague, Charles University, Faculty of Science 2020. Abstract: Two samples of historical relics of pharmaceuticals dated to the 18th century were analyzed; the first sample contained tobacco, the second laudanum, i.e., a preparation containing opium. HPLC with UV or mass detection was used for analysis. Separation of the alkaloids was performed on X Bridge® BEH C18 column using binary gradient elution. By comparison with a modern tobacco sample and on the basis of data from the literature, the presence of nicotine degradation products, especially 6-hydroxy-N-methylmyosmine, was found in the historical sample. Because the nicotine content in the historical sample was very low, it was not possible to quantify it. The analysis of opium analytes in the second historical relics revealed the main opium alkaloids (cotarnine, morphine, meconin, papaverine, and noscapine) as well as their degradation products. Main alkaloids were also quantified. The ratio of noscapine to cotarnine was determined, which according to the literature is an indicator of the age of opium pharmaceuticals. Key words: degradation, HPLC, mass spectrometry, nicotine alkaloids, opium alkaloids
|
|
|
Determination of TauroLock stability
Matejčíková, Eva ; Mravcová, Ludmila (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with the study of the anticoagulant and antimicrobial agent TauroLockTM, which is manufactured by the german company TauroPharm GmbH. Based on the background research and taking into account the equipment of analytical laboratories of the Institute of Chemistry and Technology of Environmental Protection, optimal methods of analysis were proposed and a series of experiments were carried out which focused on the determination of the active substance and degradation products of taurolidine in TauroLockTM which was stored for a certain period of time in real conditions. The main task of this work was to determine the presence of taurolidine as the active substance and formaldehyde as the undesirable product of its decomposition.
|
|
|
Computer Simulation of Quadrupole Mass Filter
Kandra, Mário ; Kolářová, Jana (referee) ; Hejč, Jakub (advisor)
At our thesis, we deal with problematics of mass spectrometry. First, we are trying to explain basic physical principles, which are used by quadrupole filter or are appearing in concomitant phenomenons. In following section we will describe theoretical nature of quadrupole mass filter, stabilization and optimalization of device. Main focus of this thesis is dedicated to simulation of quadrupole mass filter and influence of parameters on trajectory of ion movement.
|
guest ::
