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Non Realistic Visualization on Lume Pad
Chalivopulosová, Aneta ; Karas, Matej (referee) ; Milet, Tomáš (advisor)
This bachelor's thesis focuses on methods of non-realistic rendering on Lume Pad. This thesis aims to test the functionality, quality, and user-friendliness of a few selected non-realistic effects applied to 3D scenes on the holographic tablet Lume Pad. These non-realistic post-processing effects are implemented and described in this thesis. Namely, edge detection, ordered dithering, random dithering, thresholding, hatching, color shading, wobbling effect and wobble carving effect, and pencil effect. The text also describes a theoretical introduction to holography, stereoscopy, and the light field.
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Vliv teploty na oplozenost a líhnivost po krátkodobém skladování neoplozených jiker sumce velkého (Silurus glanis)
PŘIBYL, Tadeáš
The experiment validated the impact of storage of artificially spawned unfertilised eggs of European catfish on fertilization, hatching and the beginning of exogenous food intake throughout the transition from the embryonic to larval life period. The eggs from artificially spawned individuals have been used for this experiment using the induction of ovulation by the carp pituitary system. Sperm from each individual was collected by stripping using a hypodermic needle, that were partially filled with immobilising solution for sperm before artificial spawning of female individuals. Artificial stripping of fish was carried out under anaesthesia (by clove oil). Immediately after artificial hatching, samples of eggs (approximately 50 g) were put into six plastic bowls. Covered with wet cloth, bowls with eggs were placed into tempered, isolating thermo boxes with temperatures of 5, 10, 15, 20, 25 and 30 °C. Subsequently, in time intervals of 0,5, 1, 2, 3, 4, 6, 8 and 10 hours (after spawning) a small amount of eggs (approximately 50 pieces) was taken away from each temperature and put into glass beakers (with three repetitions), then the sperm was added and finally activated by adding water. In beakers with incubated non-sticking eggs and during the consequent storage of hatching embryos through temperature between 19,5-20 °C, water was changed two times a day. Approximately 4 hours after incubation, the exact number of used and fertilised eggs, was calculated. Unfertilised eggs (of white colour) and dead embryos were removed. Hatchery was assessed approximately 100 hours after fertilisation, when all living embryos had been hatched. After another 115 hours, throughout the transition from the embryonic to larval life development, live food (nauplia Artemia) was put into each bowl. Three hours after, individuals that began the food intake were calculated. The highest level of fertilisation was found in eggs stored between 0,5 and 2 hours (95,0?2,2 % - 100,0?0,0 %). The decrease in fertilisation is noticeable in all tested groups after 3 hours from stripping. Statistically significant decrease in fertilization was detected in eggs stored for 6 hours, the storage temperature did not affect the fertilization. Similar results have been maintained also in hatchery, where hatchery decreases as storage time increases. The highest level of hatchery was found in eggs stored in 25 °C (for 0,5 h 61,4?5,5 %), or more precisely 1 h 42,8?12,9 %). Hatching significantly decreases in all storage temperatures when storage time is longer than half an hour. The last parameter concerned how many percent of the individuals began the food intake. The highest level was recorded in eggs stored for half an hour (after spawning) in 25 °C (60,1?5,3 %), 30 °C (54,5?17,7 %) and 20 °C (39,0?12,7 %). On the contrary, storage temperatures 5 °C, 10 °C and 15 °C had results between 8,9?2,8 % and 35,0?18,8 %. Total mortality was detected when the storage time was more than 8 hours. It is necessary to fertilize the eggs as soon as possible (max. up to half an hour) after spawning, and to avoid storage of eggs at low temperatures (below 15 °C), to obtain viable individuals.
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Vliv teploty na udržení schopnosti oplození a líhnivosti při přechovávání neoplozených jiker u lína obecného
ANDONIU, Andreas
This theses deals with the storage length of artificially spawn of hard roes of Tench (Tinca tinca) during different temperatures at the time before the semen discharging and activation to fertilization, hatching and consequent survival of fish hatchery throughout changeover from the embryonic to larval life period (beginning of active food intake). Homogeneous assortment of hard roes obtained from hormonally induced artificial hatching of 6 spawners has been used for this experiment. Samples of hard roes were put into plastic bowls and covered, immediately after artificial hatching. Subsequently, they were placed into tempered, thermo-isolating containers with temperatures of 5, 10, 15, 20, 25 and 30 °C. In time intervals of 0.5; 1; 1,5; 2; 3; 4; 6; 8 and 10 hours, a small amount of hard roes were taken away from each temperature (estimated 50 -100 pieces) and put into dry, glass beakers (in 3 repetitions in each temperature combination and length of storage). Subsequently, the semen discharging from 6 milters was carried out and activation by water was performed. Incubation took place in non-sticking environment. During the incubation, or more precisely during the consequent storage of embryos through temperatures between 19-20.5 °C, water was changed daily. Fertilization was evaluated 48 hours after fertilizing. Hatchery was determined 48 hours after beginning of hatching of first specimen. After changeover from embryonic to larval period of ontogenetic development, living food was offered to hatching fish (artemia sp.). Thereafter, the amount of hatched fish with filled intestines was counted. Ascertained values were depicted as a percentage from the total number of seeded hard roes as well as fertilized hard roes with the use of statistic methods (two factors Anovy with the repetition). The highest level (in statistic evaluation on the importance level alfa = 0.05) of hard roe hatchery was accomplished throughout the length of possession and temperature 1 hour/ 25 °C (68.0 +- 3.1 %). The high level of hatchery was maintained by hard roes stored for 2 hours, afterwards a gradual value decrease was registered. Similarly, that was achieved with hatching parameter, where the high level of hatching was achieved with hard roes possessed for the period of 3 hours (except temperature of 30 °C), afterwards the hatchery was decreased. Pursued survival and food intake parameters of hatched fish (from the practical point of view) confirmed above stated dispositions. The high hatchery from placed hard roes was maintained for 1.5 - 3 hours (except 30 °C), thereafter there was its gradual decrease. In the time of 8 hours (temperatures 5 - 20 °C), the survival of 1.2 +- 1.8 %, was found out, with the rest, the survival was nearly zero.
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Effects of hydropeaking on the attached eggs of a rheophilic cyprinid species
BARTOŇ, Daniel
Effects of artificial water fluctuations called hydropeaking on the detachment rates of adhesive eggs were studied using a rheophlic fish (asp Leuciscus aspius) as a model species. I attempted to relate egg density to abiotic conditions of the spawning ground and identify optimal conditions for the eggs. Egg densities were also studied during spawning season when hydropeaking occurred. In the experimental setup, egg detachment rates were tested with different speeds, substrate type and exposition time and critical conditions for the eggs were assessed.
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Vliv teploty na udržení schopnosti oplození a líhnivosti při přechovávání neoplozených jiker u keříčkovce červenolemého
BORŮVKA, Vít
When hormonally induced artificial spawning of african catfish (Clarias gariepinus), was several female injected intraperitoneally in one dose preparation Ovopel at doses of 1.5 pellet × kg-1. Females were kept separately in the tanks at a temperature of 21.5 °C. All females were spawned at the same time latency 19.2 hours. Eggs from three spawned females were mixed and divided into 6 doses. Each batch was placed into thermoboxes at temperature 5 °C, 10 °C, 15 °C, 20 °C, 25 °C and 30 °C. These eggs were stored in thermoboxes and after times of storage 0.5 h, 1 h, 1.5 h, 2 h, 3 h, 4 h, 6 h, 8 h, 10h, part of the eggs (approximately 50 to 100 pieces) were taken out from each thermoboxes in three replications and was placed into individuals cups and fertilized by adding 5 drops of sperm and 20 ml of water. In these samples were subsequently observed fertilization, hatching rate and survival rate. When watching fertilization was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 2 hrs. (61.6 +- 5.81 % - 47.7 +- 1.48 %), at 10 °C in times 0.5 - 1.5 hrs. (70 +- 6.7 % - 62.1 +- 8.9 %), at 15 °C in times 0.5 - 3 hrs. (59.6 +- 9.4 % - 59.6 +- 2.9 %), at 20 °C in times 0.5 - 3 hrs. (61.4 +- 3.6 % - 56.1 +- 2.5 %), at 25 °C in times 0.5 - 4 hrs. (55.5 +- 7.2 % - 49.7 +- 9.3 %) and at 30 °C in times 0,5 - 3 hrs. (61.6 +- 10.3 % - 51.8 +- 17.8 %). When watching hatching rate was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 - 1 hrs. (28.4 +- 2.9 % - 21.1 +- 9.5 %), at 10 °C in times 0.5 - 1 hrs. (36.6 +- 17.3 % - 22.1 +- 7 %), at 15 °C in times 0.5 - 2 hrs. (34.1 +- 5.5 % - 26.9 +- 5.1 %), at 20 °C in times 0.5 - 2 hrs. (33 +- 8.2 % - 28.8 +- 1.6 %), at 25 °C in times 0.5 - 4 hrs. (31.4 +- 6.2 % - 15.3 +- 13.5 %) and at 30 °C in times 0.5 - 2 hrs. (33.1 +- 9.2 % - 21.2 +- 8 %). When watching survival rate was in individual temperature the highest values and also statistically non-significant difference ( = 0.05) achieved: at 5 °C in times of fertilization 0.5 - 1 hrs. (20.1 +- 6 % - 13 +- 3.3 %), at 10 °C in times 0.5 - 3 hrs. (19.8 +- 15.31 % - 3.1 +- 3 %), at 15 °C in times 0.5 - 6 hrs. (23.3 +- 9 % - 5 +- 2.8 %), at 20 °C in times 0.5 - 2 hrs. (22.4 +- 1.9 % - 15.1 +- 5.2 %), at 25 °C in times 0.5 - 4 hrs. (18.7 +- 4.4 % - 4.1 +- 1.9 %) and at 30 °C in times 0.5 - 1.5 hrs. (26.2 +- 5.5 % - 21.4 +- 6.8 %). Suitable temperatures for the storage of unfertilized eggs after spawning are two hours before fertilization at temperatures from 15 to 30 °C. Other suitable temperatures which are useful for storage are temperatures 15 to 25 °C, for preservation after 3 hrs. and longer after fertilization.
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Influence of experimental conditions on hatching of diapausing stages of the cladoceran Daphnia obtusa
Sailerová, Martina ; Petrusek, Adam (advisor) ; Vaníčková, Ivana (referee)
Diapause is often an adaptation for survival during periods of harsh environmental conditions. Some diapausing stages do not terminate the dormancy once the favourable conditions are restored. Such prolonged diapause may be enforced by environment if a diapausing stage cannot be reached by the cues inducing termination of dormancy. However, it may also be an advantageous bet-hedging strategy to allow only a fraction of dormant stages produced in any given season to hatch the next time conditions become favourable. I tested whether such strategy can be observed in hatching patterns of dormant eggs of Daphnia obtusa - a cladoceran occurring in small Central European temporary waters. I investigated the influence of intensity of illumination on hatching success, and effect of isolating the eggs encased in ephippia from the sediment. Fraction of eggs terminating diapause, fraction of embryos successfully leaving the egg membranes, and timing of the response were assessed at 15 ˚C under four intensities of illumination (100% = 35µmol.m2 .s-1 , 75%, 50%, 25%; photoperiod 12h light: 12h dark) and in complete darkness for 21 days. My results support previous suggestions that there is no genetically-fixed bet-hedging strategy in D. obtusa. I observed high proportion of eggs which terminated diapause in all...
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Feeding of early fry of Siberian sturgeon (Acipenser baeri) with the use bio-encapsulated nauplii of Artemia r. (Artemia)
STARÝ, Jakub
The aim of the my work is finding of effectiveness of enrichment of Artemia salina by different substances. We watched efficiency of substances to growth and mortality of larvae of Siberian sturgeon. The larvae were kept in aquariums (18), which were divided into two groups. One of them was fed by artemia one week and five weeks by dry granulated feed and the second was fed by artemia three weeks and three weeks by dry granulated feed. Then all of aquariums were divided into three and three groups (three aquariums for one group), which was fed by artemia enriched by different substances (group without enrichment-control, group enriched with preparation DHA and a third group was enriched with preparation Spresso). For proper breeding we had the temperature of water at 20 °C+.After regular measurement of temperature in the morning and in the evening were measured average values morning x=22,4 °C and average values evening s = 22.9 ° C. The attempt proved double growth in the group which was fed by artemia one week, but at the expense of higher mortality. The group which was fed by Artemia enriched by product Spresso showed in both groups the best growth and weight characteristics. In conclusion we can say that the enrichment by product Spresso is better than feeding by artemia without enrichment.
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Resistance of some species of fish embryos to cryoprotectants at low temperatures
ALDORF, Milan
Different cryoprotectant media for cryopreservation of embryos has been tested on model species, i.e. common carp (Cyprinus carpio) and common tench (Tinca tinca). The aim of the study was to obtain such cryoprotectants, which will be acceptable for freezing embryos up to the temperature {--}196 oC. Cryoprotectants of 10 % and 20 % methanol or 10 % and 20 % glycerin have been tested on the tench for 21 minutes of incubation on embryos of four stages, meaning at 11, 17, 23 and 29-hrs after activation of gametes. The results showed that the tench embryos were most resistant either to low temperature and or to the application of cryoprotectants in the stage of 29-hrs post gametes activation. On the other hand lower resistances were obtained in the stage of 11-hrs post gamete activation. Embryos of carp 2, 6, 22, 24 and 42-hrs after gametes activation at temperature 18 and 22 oC have been used for testing of concentration series of cryoprotectant methanol and two solutions marked VS1 and VS2 after previous disruption of egg envelope in enzyme alcalaze solution. Results showed linear decreasing resistance of embryos depending on increasing concentration of cryoprotectant methanol. Hatching success even at highest concentration of solution VS1 and VS2 has not declined below 70 %. Achieved results with solution VS2 have been subsequently used for freezing of carp embryos by special methods in cryobiology {--} vitrification. First results showed up to 4 % success of survival after freezing of embryos at {$-$}196 oC.
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