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Studium celogenomové variability lidského cytomegaloviru.
Dvořák, Jan ; Tachezy, Ruth (advisor) ; Roubalová, Kateřina (referee)
This work is part of a project focused on the study of the variability of human cytomegalovirus (HCMV) among clinical isolates with the aim to map the geographical distribution of HCMV genotypes, reveal the relationships between genotypes and the severity of HCMV-associated diseases, and identify regions in the HCMV genome with a potential for use as diagnostic and therapeutic targets. Attention was paid to the development of the methodology for the preparation of the material for next-generation sequencing (NGS) from HCMV clinical isolates and evaluation of the obtained sequencing data. Blood and urine samples collected from hematopoietic stem cell transplantat recipients and congenitally infected children were analyzed. Samples suitable for NGS were sequenced by the Illumina platform and sequences were created by de novo assembly followed by mapping assembly. Urine samples in comparison to blood samples had higher yield of material for NGS. Of the samples positive for HCMV DNA (7 of 50) after amplification in the cell cultures, only one sample had high purity of the viral DNA (98%) while six samples had purity of less than 7%. The sample containing 98% of the viral DNA was fully sequenced and the sequence was compared to the sequences of other clinical isolates from Belgium in 11 polymorphic...
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Intraspecific variability in plant antioxidant systems and its impact on drought tolerance
Jelénková, Iva ; Kočová, Marie (advisor) ; Zelenková, Sylva (referee)
Drought is one of the major factors limiting agricultural production. Reactive oxygen species (eg. superoxide radical, hydroxyl radical, hydrogen peroxide) generated during stress in plant cells activate antioxidant mechanisms, which scavenge those toxic substances. The positive correlation between activity of enzymatic (eg. catalase, superoxide dismutase, glutathione reductase, peroxidases) and nonenzymatic (eg. glutathione, ascorbic acid) antioxidants and stress tolerance was confirmed in various studies. Both interspecific and intraspecific variability was found in activities of antioxidant systems of plants. Higher activity was also observed in F1 generation in relation to positive heterosis, or in plants treated with supportive substances such as abscisic acid, brassinosteroids and nitrogen. The increase in the activity of antioxidants was also proved in relation to the ploidy level or to intensity of drought. It was observed the positive effect of previous drought exposition on tolerance to other abiotic stressors. The complex response of plants to stress factors depends on many other internal and external factors.
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The importance of time, space and genotype for the intensity of plant-soil feedback
Vondráková, Zuzana ; Münzbergová, Zuzana (advisor) ; Janoušková, Martina (referee)
Plant-soil feedback is an important mechanism in plant communities affecting their species composition. Understanding plant-soil feedback is crucial for describing the interactions between plant communities and their soil communities. The effects of plant-soil feedback can be positive, negative or neutral and because of these changes plants are either coexisting or competing. Plant-soil feedback is often studied within plant succession, plant invasion and plant dominance in a plant community. In these cases, the nature and strength of influence of each species is studied. Despite the large number of previous studies on plant-soil feedback, very little is known about the temporal and spatial changes of the intensity of plant-soil feedback and the intraspecific variability in plant-soil feedback. The aim of my work was to observe the temporal changes in the plant-soil feedback of a species growing in soil which was conditioned by the same or other plant species. Another goal was to explore spatial changes in plant-soil feedback. The last objective was to test differences in the intensity of plant-soil feedback among different genotypes of one species. The results of my work demonstrated that the intensity of intraspecific plant-soil feedback of Rorippa austriaca is negative and increases with...
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Identifying a genetic predisposition affecting sports performance, focusing on anaerobic activity of skeletal muscle activity
Balkó, Iva ; Kohlíková, Eva (advisor) ; Šimonek, Jaromír (referee) ; Zvonař, Martin (referee)
Title Identifying of genetic predisposition affecting sports performance focusing on anaerobic activity of skeletal muscle activity Problem Inborn predispositions of every individual are mainly influenced by the inherited genetic information in their DNA with the addition of external environmental factors. Based on the high variability of the inborn predictions in the form of phenotypic differences in the anatomy or physiology of an individual, it can be deduced on the connection between genetics and a specific sport talent. According to current scientific knowledge, anaerobic performance of the skeletal muscle tissue seems to be more genetically influenced. Anatomic and functional properties of the skeletal muscle are mainly influenced by genes, which have effect on the muscle fibre structure, blood storage, metabolism, neurotransmission, and muscle regeneration, etc. Fencing is a complex sport discipline where predispositions to speed and dynamic force play a key role. The sport performance in fencing, regardless of the type of weapon, is directly dependent on the interactions between the reaction of the fencer to the surrounding stimuli (visual, tactile), accuracy, tactical and technical skills, level of anticipation and overall physical a psychological readiness of the fencer. This work is...
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The comparison of oat varieties from the point of view of yield and resistence to diseases
FUKSOVÁ, Eliška
The purpouse of the thesis was compared the comparison in the yields of hulled and naked verifield varieties and new breeding genotypes of oats. The research was conducted in the breeding station Selgen Krukanice and and it was mainly focused on the main elements of yieĺd - number of plants, cuttings, and lat per m2, toatal grain yield, the number of grains per panicle, and the quality of grain - weight of thousand grains, density and share of fixed plugs. Results of the main elements of yield was average at all genotypes of oat, qualitative parametres wasslighty below average, the reason wast dry and warm weather. Also was found good health of oats, cause of weather, that was not favorable for disease development.
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Identifying a genetic predisposition affecting sports performance, focusing on anaerobic activity of skeletal muscle activity
Balkó, Iva ; Kohlíková, Eva (advisor) ; Šimonek, Jaromír (referee) ; Zvonař, Martin (referee)
Title Identifying of genetic predisposition affecting sports performance focusing on anaerobic activity of skeletal muscle activity Problem Inborn predispositions of every individual are mainly influenced by the inherited genetic information in their DNA with the addition of external environmental factors. Based on the high variability of the inborn predictions in the form of phenotypic differences in the anatomy or physiology of an individual, it can be deduced on the connection between genetics and a specific sport talent. According to current scientific knowledge, anaerobic performance of the skeletal muscle tissue seems to be more genetically influenced. Anatomic and functional properties of the skeletal muscle are mainly influenced by genes, which have effect on the muscle fibre structure, blood storage, metabolism, neurotransmission, and muscle regeneration, etc. Fencing is a complex sport discipline where predispositions to speed and dynamic force play a key role. The sport performance in fencing, regardless of the type of weapon, is directly dependent on the interactions between the reaction of the fencer to the surrounding stimuli (visual, tactile), accuracy, tactical and technical skills, level of anticipation and overall physical a psychological readiness of the fencer. This work is...
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Markers of pine physiological state in relation to genetic variability
Šafránková, Anna ; Albrechtová, Jana (advisor) ; Prášil, Ilja (referee)
Breeding of coniferous trees in the Czech Republic is undergoing an important development during last decades, especially thanks to molecular-genetic methods, which refine and simplify mapping of tree genotypes and the selection of superior genotypes. Recently, in the Czech Republic superior genotypes are selected based on forestry parameters (tree height, trunk diameter, and timber quality) what does not always correlate with the ability of a tree to resist abiotic and biotic stresses. Recently, there is an effort to include in the breeding also physiological parameters and select superior genotypes using nonspecific stress indicators, which are able to correspond better to tree fitness than the forestry growth parameters. The present thesis deals with genotypes of Scots pine (Pinus sylvestris L.) growing in seed orchards Doubrava and Silov in the Pilsen region in the Czech Republic. Seed orchards are tree plantations, which serve as a reserve of the genetically valuable reproduction material, they are parts of breeding programs. Pine needles were collected in July 2015 and analyzed for non-specific stress indicators - photosynthetic pigments, phenolics, lignin, cellulose and proline contents and leaf reflectance and fast chlorophyll fluorescence measurements. First objective of the present thesis...
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The importance of time, space and genotype for the intensity of plant-soil feedback
Vondráková, Zuzana ; Münzbergová, Zuzana (advisor) ; Janoušková, Martina (referee)
Plant-soil feedback is an important mechanism in plant communities affecting their species composition. Understanding plant-soil feedback is crucial for describing the interactions between plant communities and their soil communities. The effects of plant-soil feedback can be positive, negative or neutral and because of these changes plants are either coexisting or competing. Plant-soil feedback is often studied within plant succession, plant invasion and plant dominance in a plant community. In these cases, the nature and strength of influence of each species is studied. Despite the large number of previous studies on plant-soil feedback, very little is known about the temporal and spatial changes of the intensity of plant-soil feedback and the intraspecific variability in plant-soil feedback. The aim of my work was to observe the temporal changes in the plant-soil feedback of a species growing in soil which was conditioned by the same or other plant species. Another goal was to explore spatial changes in plant-soil feedback. The last objective was to test differences in the intensity of plant-soil feedback among different genotypes of one species. The results of my work demonstrated that the intensity of intraspecific plant-soil feedback of Rorippa austriaca is negative and increases with...
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Studium celogenomové variability lidského cytomegaloviru.
Dvořák, Jan ; Tachezy, Ruth (advisor) ; Roubalová, Kateřina (referee)
This work is part of a project focused on the study of the variability of human cytomegalovirus (HCMV) among clinical isolates with the aim to map the geographical distribution of HCMV genotypes, reveal the relationships between genotypes and the severity of HCMV-associated diseases, and identify regions in the HCMV genome with a potential for use as diagnostic and therapeutic targets. Attention was paid to the development of the methodology for the preparation of the material for next-generation sequencing (NGS) from HCMV clinical isolates and evaluation of the obtained sequencing data. Blood and urine samples collected from hematopoietic stem cell transplantat recipients and congenitally infected children were analyzed. Samples suitable for NGS were sequenced by the Illumina platform and sequences were created by de novo assembly followed by mapping assembly. Urine samples in comparison to blood samples had higher yield of material for NGS. Of the samples positive for HCMV DNA (7 of 50) after amplification in the cell cultures, only one sample had high purity of the viral DNA (98%) while six samples had purity of less than 7%. The sample containing 98% of the viral DNA was fully sequenced and the sequence was compared to the sequences of other clinical isolates from Belgium in 11 polymorphic...
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Intraspecific variability in plant antioxidant systems and its impact on drought tolerance
Jelénková, Iva ; Kočová, Marie (advisor) ; Zelenková, Sylva (referee)
Drought is one of the major factors limiting agricultural production. Reactive oxygen species (eg. superoxide radical, hydroxyl radical, hydrogen peroxide) generated during stress in plant cells activate antioxidant mechanisms, which scavenge those toxic substances. The positive correlation between activity of enzymatic (eg. catalase, superoxide dismutase, glutathione reductase, peroxidases) and nonenzymatic (eg. glutathione, ascorbic acid) antioxidants and stress tolerance was confirmed in various studies. Both interspecific and intraspecific variability was found in activities of antioxidant systems of plants. Higher activity was also observed in F1 generation in relation to positive heterosis, or in plants treated with supportive substances such as abscisic acid, brassinosteroids and nitrogen. The increase in the activity of antioxidants was also proved in relation to the ploidy level or to intensity of drought. It was observed the positive effect of previous drought exposition on tolerance to other abiotic stressors. The complex response of plants to stress factors depends on many other internal and external factors.
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