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Gene engineering methods in Leishmania research
Kriegová, Gabriela ; Bečvář, Tomáš (advisor) ; Arbonová, Lenka (referee)
Gene engineering methods are now increasingly used in the study of leishmania. These modern DNA technologies make it possible to manipulate with the genome of organisms, allowing it to be altered and repaired. The most important methods include CRISPR/Cas9, RNA interference (RNAi), dimerizable Cre recombinase (DiCre), plasmid shuffle and gene tagging. The choice of method depends on whether essential or non-essential genes are being studied as well as the reason of the study. These methods investigate leishmania using a reverse genetics approach that allows the phenotype to be studied. This work summarizes the current knowledge on the use of gene engineering methods in Leishmania, the necessary modifications of the procedures and discusses their limitations and advantages.
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Critical evaluation of potential of harvested product proteins in selected crops from perspective of their use in food and biotechnological applications
BUCKOVÁ, Pavlína
This diploma thesis deals with plant proteins. Literary review discusses the properties of the major proteins in selected agricultural crops (wheat, barley, oats, corn, peas, soybeans, canola, potato, Jerusalem artichoke) and their use in food, diet, biotechnology and other industries. The diploma thesis was also assessing the potential production of proteins in these crops on the basis of literature and available statistical data. Data is organized into tables and graphs
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