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Musk compounds in water ecosystem
Guryčová, Hana ; Čáslavský, Josef (referee) ; Vávrová, Milada (advisor)
Synthetic fragrances or MUSK compounds are artificialy synthetised organic substances, used as fragrant constituents in various homecare products, such as cosmetics, detergents, air fresheners etc. The aim of diploma thesis was the quantification of some selected MUSK compounds in the influent and efluent of the water treatment plants.The method of quantification of MUSK compounds in organic matrix was also optimised during the research. Two modifications of solid phase microextraction were tested and evaluated. The analytes were identified and quantified by gas chromatography - mass spectrometry (GC/MS). The results were used to evaluate the amount of MUSK compounds in sewage waters and the ability of various water treatment plants to eliminate this kind of pollution from the water during the treatment process.
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Bisphenol A in water ecosystem
Nohelová, Gabriela ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with Bisphenol A, especially with its impact on the aquatic ecosystem. Information about its properties, production and current use are summarized here. Its harmful impact on the environment, especially on the aquatic ecosystem and the human body is characterized. Also the methods of its degradation within the aquatic environment have been described. A summary of the options of a determination of Bisphenol A in water samples is incorporated and the method of gas chromatography with mass spectrometry (GC/TOF MS) and comprehensive two-dimensional gas chromatography with mass spectrometry (GCxGC/TOF-MS) is compared in the experimental part. Analytical determination precedes the isolation of the analyte from the water samples by solid phase extraction (SPE) using SupelcleanTM ENVITM - 18 and derivatization using the silylation reagent, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The result of this work is the analysis of a series of real samples from wastewater treatment plants Brno Modřice and Luhačovice by a two-dimensional gas chromatography with mass spectrometry (GCxGC/TOF-MS).
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Analýza sexuálního feromonu ovocných mušek rodu Ceratitis fasciventris, Ceratitis anonae a Ceratitis rosa
Faťarová, Mária ; Kalinová, Blanka (advisor) ; Rezek, Jan (referee)
Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa are polyphagous agricultural pests originating from African continent. Their behaviour is heavily altered by pheromones. Insect chemical communication channels are species-specific, represents taxonomic and reproduction barriers. Taxonomy of this group (so-called Ceratitis FAR complex) is unclear. Therefore new chemical approaches along with genetic tests for identification of entities within the cryptic species FAR complex are being developed. To study multi-component mixtures of male volatiles originating from the three mentioned fruit fly species, comprehensive two-dimensional gas chromatography with time- of-flight mass spectrometer (GC×GC-TOFMS) was used. A number of compounds were identified, out of which 23 were found to be distributed and shared among the studied species, 11 out of these were present in all three species. Analyses of male pheromone volatiles, using gas chromatography combined with electroantennographic detector (GC-EAD) revealed 4 common compounds with antennal activity shared among three studied species: methyl (E)-hex-3-enoate, 6-methylhept-5-en-2- one, linalool, and methyl (2E,6E)-farnesoate. The species-specific EAD active compounds in C. fasciventris were esters of isomers of hexenoic acid, whereas isomers of...
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Bisphenol A in water ecosystem
Nohelová, Gabriela ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
This diploma thesis deals with Bisphenol A, especially with its impact on the aquatic ecosystem. Information about its properties, production and current use are summarized here. Its harmful impact on the environment, especially on the aquatic ecosystem and the human body is characterized. Also the methods of its degradation within the aquatic environment have been described. A summary of the options of a determination of Bisphenol A in water samples is incorporated and the method of gas chromatography with mass spectrometry (GC/TOF MS) and comprehensive two-dimensional gas chromatography with mass spectrometry (GCxGC/TOF-MS) is compared in the experimental part. Analytical determination precedes the isolation of the analyte from the water samples by solid phase extraction (SPE) using SupelcleanTM ENVITM - 18 and derivatization using the silylation reagent, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The result of this work is the analysis of a series of real samples from wastewater treatment plants Brno Modřice and Luhačovice by a two-dimensional gas chromatography with mass spectrometry (GCxGC/TOF-MS).
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Musk compounds in water ecosystem
Guryčová, Hana ; Čáslavský, Josef (referee) ; Vávrová, Milada (advisor)
Synthetic fragrances or MUSK compounds are artificialy synthetised organic substances, used as fragrant constituents in various homecare products, such as cosmetics, detergents, air fresheners etc. The aim of diploma thesis was the quantification of some selected MUSK compounds in the influent and efluent of the water treatment plants.The method of quantification of MUSK compounds in organic matrix was also optimised during the research. Two modifications of solid phase microextraction were tested and evaluated. The analytes were identified and quantified by gas chromatography - mass spectrometry (GC/MS). The results were used to evaluate the amount of MUSK compounds in sewage waters and the ability of various water treatment plants to eliminate this kind of pollution from the water during the treatment process.
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