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Utilization of contacts phytotoxicity tests for evaluation of energetic byproducts
Jozífková, Zuzana ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The energetic by-products are a part of energetic wastes which are residues of fuels combustion or also the residues of flue-gas cleaning. Their following use could impact the terrestrial and subsequently also aquatic ecosystems. For these reasons it is necessary to evaluate with their possible ecotoxicity. In this diploma work the samples of energy products were subjected to ecotoxicological testing via contact phytotoxicity tests on Embryophyta. Water leaches of these samples were tested in order of comparison of the two testing methods. To test the water leaches of energy by-products the root growth inhibition test of five selected plant species were used; white mustard (Sinapis alba), kohlrabi oilseed rape (Brassica napus), turnip kohlrabi rape (Brassica rapa), garden lettuce (Lactuca sativa) and oats (Avena sativa). For the evaluation of ecotoxicity in contact arrangement the following phytotoxicity tests were selected: germination and growth of higher plants, brassica oilseed rape (Brassica napus) and oats (Avena sativa). Furthermore, the limit concentrations of waste at the plant Brassica napus were tested and screening tests poppy germination of lettuce (Lactuca sativa) were performed. On the basis of obtained results ecotoxicity of energetic by-product was assessed and needs of solid matrices testing in contact arrangement discussed.
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Ecotoxicological tests and their aplication for ecotoxicity evaluation of energetic byproducts
Ballnérová, Petra ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The energetic byproducts are a part of energetic wastes which are residues of fuels combustion or also the residues of flue-gas cleaning. Their following application can impact the terrestrial and subsequently also aquatic ecosystems. With respect to these reasons it is necessary to deal with their possible ecotoxicity. Within the framework of this diploma thesis water leaches from the byproducts’ samples were prepared and subsequently ecotoxicaly tested. Three alternative toxicity tests on aquatic organisms were used: Thamnotoxkit FTM and a Rapidtoxkit FTM on sense organisms Thamnocephalus platyurus and Daphtoxkit FTM on sense organisms Daphnia magna. Standard tests of fytotoxicity were also used; Sinapis alba root growth inhibition test and Lemna minor growth inhibition test. The values of EC50, LC50 and IC50 were determined and ecotoxicity of energetic byproducts was evaluated.
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UTILIZATION OF ALTERNATIVE TESTS OF BIOTOXICITY FOR DETERMINATION INADVISABILITY OF EXISTING AND/OR POTENTIONAL POLUTANTS
Vašulková, Lucie ; Zlámalová Gargošová, Helena (referee) ; Bednařík, Karel (advisor)
This thesis is focused on attestation of ecotoxicity of octachlorstyrene (OCS), persistent organic pollutant (POP), by biological tests of toxicity. The toxicity of OCS was determined by using two kinds of alternative tests applied on fresh water organisms. First of them, Thamnotoxkit, is based on observation of mobility, immobilization or even killing of crustacean Thamnocephalus platyurus. The second one, Daphnotoxkit, applies crustacean Daphnia magna. For purposes of comparison, a standard test of toxicity – so called test of root growth inhibition of Sinapis alba and two fytotests – test of growth inhibition of Lemna minor and test of root growth inhibition of Allium cepa were performed as well.
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Utilization of contacts phytotoxicity tests for evaluation of energetic byproducts
Jozífková, Zuzana ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The energetic by-products are a part of energetic wastes which are residues of fuels combustion or also the residues of flue-gas cleaning. Their following use could impact the terrestrial and subsequently also aquatic ecosystems. For these reasons it is necessary to evaluate with their possible ecotoxicity. In this diploma work the samples of energy products were subjected to ecotoxicological testing via contact phytotoxicity tests on Embryophyta. Water leaches of these samples were tested in order of comparison of the two testing methods. To test the water leaches of energy by-products the root growth inhibition test of five selected plant species were used; white mustard (Sinapis alba), kohlrabi oilseed rape (Brassica napus), turnip kohlrabi rape (Brassica rapa), garden lettuce (Lactuca sativa) and oats (Avena sativa). For the evaluation of ecotoxicity in contact arrangement the following phytotoxicity tests were selected: germination and growth of higher plants, brassica oilseed rape (Brassica napus) and oats (Avena sativa). Furthermore, the limit concentrations of waste at the plant Brassica napus were tested and screening tests poppy germination of lettuce (Lactuca sativa) were performed. On the basis of obtained results ecotoxicity of energetic by-product was assessed and needs of solid matrices testing in contact arrangement discussed.
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Ecotoxicological tests and their aplication for ecotoxicity evaluation of energetic byproducts
Ballnérová, Petra ; Vávrová, Milada (referee) ; Zlámalová Gargošová, Helena (advisor)
The energetic byproducts are a part of energetic wastes which are residues of fuels combustion or also the residues of flue-gas cleaning. Their following application can impact the terrestrial and subsequently also aquatic ecosystems. With respect to these reasons it is necessary to deal with their possible ecotoxicity. Within the framework of this diploma thesis water leaches from the byproducts’ samples were prepared and subsequently ecotoxicaly tested. Three alternative toxicity tests on aquatic organisms were used: Thamnotoxkit FTM and a Rapidtoxkit FTM on sense organisms Thamnocephalus platyurus and Daphtoxkit FTM on sense organisms Daphnia magna. Standard tests of fytotoxicity were also used; Sinapis alba root growth inhibition test and Lemna minor growth inhibition test. The values of EC50, LC50 and IC50 were determined and ecotoxicity of energetic byproducts was evaluated.
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UTILIZATION OF ALTERNATIVE TESTS OF BIOTOXICITY FOR DETERMINATION INADVISABILITY OF EXISTING AND/OR POTENTIONAL POLUTANTS
Vašulková, Lucie ; Zlámalová Gargošová, Helena (referee) ; Bednařík, Karel (advisor)
This thesis is focused on attestation of ecotoxicity of octachlorstyrene (OCS), persistent organic pollutant (POP), by biological tests of toxicity. The toxicity of OCS was determined by using two kinds of alternative tests applied on fresh water organisms. First of them, Thamnotoxkit, is based on observation of mobility, immobilization or even killing of crustacean Thamnocephalus platyurus. The second one, Daphnotoxkit, applies crustacean Daphnia magna. For purposes of comparison, a standard test of toxicity – so called test of root growth inhibition of Sinapis alba and two fytotests – test of growth inhibition of Lemna minor and test of root growth inhibition of Allium cepa were performed as well.
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