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Fluorimetric Determination of Scandium
Holubová, Zuzana ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The submitted thesis deals within the sensitive fluorimetric determination of scandium with a new reagent 8-Hydroxyquinoline-5-sulphonic acid. All important factors such as time, pH, reagent concentration, buffers, surfactants and selected interferents have been studied with respect to the selectivity, sensitivity, precision and detection limit on the determination by using classic fluorescence spectra and their first derivation. This reagent was also used for the determination of scandium in real waters. The new reagent was shortly compared to morin and lumogallion when used for scandium.
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Monitoring of the occurence of Cyanobacteria in the Seč water resevoir by analytical methods
Štangelová, Pavla ; Tesařová, Eva (advisor) ; Cajthaml, Tomáš (referee)
Cyanobacteria are one of the oldest living organisms on the planet Earth. They are able to survive in different conditions under various conditions, almost in all habitats. The growth of cyanobacteria is affected by the amount of nutrients present in the environment, especially nitrogen and phosphorus, and by the climatic conditions. In larger quantities cyanobacteria began to spread in past few decades due to human activities. As a result of excessive use of fertilizers and washing nutrients from the soil is human-caused eutrophication and expansion of the so-called "water bloom". The presence of cyanobacteria in water is monitored using various analytical methods. The occurrence of cyanobacteria on the water reservoir Seč was determined by fluorimetric detection of pigment c-phycocyanin in samples of natural waters and compared with chlorophyll a content in these samples. Key words: cyanobacteria, c-phycocyanin, chlorophyll-a, fluorimetry
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Determination of Nadroparine in Fraxiparine injection solution using flow techniques of anlysis
Miklošinová, Aneta ; Hraníček, Jakub (advisor) ; Křížek, Tomáš (referee)
This thesis was focused on a determination of nadroparin using sequential injection analysis and flow injection analysis with fluorimetric and spectrophotometric detection. The principle of determination was based on the interaction of nadroparin with phenothiazine dye. Methylene blue was used as phenothiazine dye. The determination was performed on a laboratory made FIA instrument and on the commercial SIA instrument. Optimal conditions for SIA were concentration of dye 6 ∙ 10-5 mol dm-3 , injected volume 100 µl, flowrate 1,5 ml min-1 , for FIA: concentration of dye 3 ∙10-5 mol dm-3 flowrate 2 ml ∙ min-1 , injected volume= 100 µl, for fluorimetric detection was sensivity of the detector 1000 V, Emission was measured by 2 nm and excitation wavelenght 621 nm. For spectrophotometric detection, absorbance was detected at the absorption maximum wavelength. Repeatability ranged from 2.01 to 2.85%. The limit of detection for FIA was 0.05-0.09 IU ml-1 , for SIA were limits of detection in range 0,25 - 1,63 IU ml-1 , limits of quantification in range 0,83 - 5,44 and linear range was from 0,5 - 20 IU ml-1 . The limits of detection, limits of determination and the linear range could be corrected for the SIA by the injected volume of phenothiazine dye.
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Monitoring of the occurence of Cyanobacteria in the Seč water resevoir by analytical methods
Štangelová, Pavla ; Tesařová, Eva (advisor) ; Cajthaml, Tomáš (referee)
Cyanobacteria are one of the oldest living organisms on the planet Earth. They are able to survive in different conditions under various conditions, almost in all habitats. The growth of cyanobacteria is affected by the amount of nutrients present in the environment, especially nitrogen and phosphorus, and by the climatic conditions. In larger quantities cyanobacteria began to spread in past few decades due to human activities. As a result of excessive use of fertilizers and washing nutrients from the soil is human-caused eutrophication and expansion of the so-called "water bloom". The presence of cyanobacteria in water is monitored using various analytical methods. The occurrence of cyanobacteria on the water reservoir Seč was determined by fluorimetric detection of pigment c-phycocyanin in samples of natural waters and compared with chlorophyll a content in these samples. Key words: cyanobacteria, c-phycocyanin, chlorophyll-a, fluorimetry
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Fluorimetric Determination of Scandium
Holubová, Zuzana ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The submitted thesis deals within the sensitive fluorimetric determination of scandium with a new reagent 8-Hydroxyquinoline-5-sulphonic acid. All important factors such as time, pH, reagent concentration, buffers, surfactants and selected interferents have been studied with respect to the selectivity, sensitivity, precision and detection limit on the determination by using classic fluorescence spectra and their first derivation. This reagent was also used for the determination of scandium in real waters. The new reagent was shortly compared to morin and lumogallion when used for scandium.
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