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Design of Excimer Laser for Ophthalmology
Monsportová, Kateřina ; John, David (referee) ; Rubínová, Dana (advisor)
The theme of this master thesis is a design of excimer laser for ophthalmology. The main goal is to depict a medical device with innovative appearance with a special emphasis on the ergonomics of the surgeon's and optometrist's work environment with a regard to the elimination of negative impressions on the patient as a result of the surgery and the creation of a comfortable and suitably shaped bed. The thesis is based on an analysis of the current market and a questionnaire survey. The final product finds its application in specialized eye clinics and in hospital ophthalmology departments.
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Design of Excimer Laser for Ophthalmology
Monsportová, Kateřina ; John, David (referee) ; Rubínová, Dana (advisor)
The theme of this master thesis is a design of excimer laser for ophthalmology. The main goal is to depict a medical device with innovative appearance with a special emphasis on the ergonomics of the surgeon's and optometrist's work environment with a regard to the elimination of negative impressions on the patient as a result of the surgery and the creation of a comfortable and suitably shaped bed. The thesis is based on an analysis of the current market and a questionnaire survey. The final product finds its application in specialized eye clinics and in hospital ophthalmology departments.
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Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
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Utilization of protein radical foootprinting for stuctural biology
Polák, Marek ; Novák, Petr (advisor) ; Junková, Petra (referee)
(In English) The reaction of highly reactive oxygen radicals with protein solvent-accessible residues can be utilized to map protein landscape. Fast photochemical oxidation of proteins (FPOP) is an MS- based technique, which utilizes highly reactive radical species to oxidize proteins and map protein surface or its interactions with their interaction partners. In this work, FPOP was employed to study protein-DNA interactions. First, a full-length of FOXO4-DBD was successfully expressed and purified. The ability of the protein to bind its DNA-response element was verified by electrophoretic and MS-based techniques, respectively. Optimal experimental conditions were achieved to oxidize the protein itself and in the presence of DNA, respectively. Oxidized samples were analyzed by bottom-up and top-down approach. In the bottom-up experiment, modification of individual residues was precisely located and quantified. Different extend of modification was observed for protein alone and in complex with DNA. To avoid experimental artifacts analyzing multiply oxidized protein, standard bottom up approach was replaced by a progressive top-down technology. Only a singly oxidized protein ion was isolated, and further fragmented by collision-induced dissociation (CID) and electron-capture dissociation (ECD),...
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