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Use of the QuEChERS method for the analysis of fluoroquinolone antibiotics in soil
Brabcová, Kristýna ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
The content of this master's thesis is an application of frequently used extraction method QuEChERS on soil or other samples like litter or manure containing fluoroquinolone antibiotics. In the theoretical part of this thesis are chapters describing drugs, antibiotics and antibiotic resistance. Fluoroquinolones are described in more details with their properties, appearance and behaviour in the environment. The last chapter is focused on analytical methods. In the experimental part of this thesis is chosen in accordance with available publications the most suitable extraction method. This method is tested and optimized on soil samples. The optimized method is applied on the real soil and poultry litter samples to determine the amount of ciprofloxacine and enrofloxacine. This extraction method is used on other fluoroquinolone antibiotics (norfloxacine, ofloxacine, pefloxacine, moxifloxacine) in soil. All of the samples were analyzed by LC-MS.
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Determination of antibiotic residues in soil
Kaprinay Bréda, Boglárka ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
This master‘s thesis is focused on the optimization of sample preparation and analytical method for the determination of fluoroquinolone. The theoretical part describes the principles of analysis in soil samples, environmental aspects of antibiotic resistance, characteristics of UPLC-MS / MS methods, description of quinolone. The experimental part deals with the identification and determination of fluoroquinolone substance (ciprofloxacin, enrofloxacin) in soil in which manure from poultry litter was incorporated using liquid chromatography with mass spectrometry (MS / MS, triple quadrupole). Internal standard and standard addition methods were used to quantify ciprofloxacin and enrofloxacin. The amount of CIP in the samples ranged from 0.2030 to 12.7088 µg·g-1 and for ENR from 0.2537 to 10.0224 µg·g-1.
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Use of the QuEChERS method for the analysis of fluoroquinolone antibiotics in soil
Brabcová, Kristýna ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
The content of this master's thesis is an application of frequently used extraction method QuEChERS on soil or other samples like litter or manure containing fluoroquinolone antibiotics. In the theoretical part of this thesis are chapters describing drugs, antibiotics and antibiotic resistance. Fluoroquinolones are described in more details with their properties, appearance and behaviour in the environment. The last chapter is focused on analytical methods. In the experimental part of this thesis is chosen in accordance with available publications the most suitable extraction method. This method is tested and optimized on soil samples. The optimized method is applied on the real soil and poultry litter samples to determine the amount of ciprofloxacine and enrofloxacine. This extraction method is used on other fluoroquinolone antibiotics (norfloxacine, ofloxacine, pefloxacine, moxifloxacine) in soil. All of the samples were analyzed by LC-MS.
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Determination of antibiotic residues in soil
Kaprinay Bréda, Boglárka ; Zlámalová Gargošová, Helena (referee) ; Mravcová, Ludmila (advisor)
This master‘s thesis is focused on the optimization of sample preparation and analytical method for the determination of fluoroquinolone. The theoretical part describes the principles of analysis in soil samples, environmental aspects of antibiotic resistance, characteristics of UPLC-MS / MS methods, description of quinolone. The experimental part deals with the identification and determination of fluoroquinolone substance (ciprofloxacin, enrofloxacin) in soil in which manure from poultry litter was incorporated using liquid chromatography with mass spectrometry (MS / MS, triple quadrupole). Internal standard and standard addition methods were used to quantify ciprofloxacin and enrofloxacin. The amount of CIP in the samples ranged from 0.2030 to 12.7088 µg·g-1 and for ENR from 0.2537 to 10.0224 µg·g-1.
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