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Obsah organického uhlíku v orniční a podorniční vrstvě půdy v porostech energetických trav
BLÁHA, David
The study aimed to determine the total content of organic carbon in the topsoil and subsoil layer of energy grasses (Dactylis glomerata, Elymus elongatus subsp. ponticus cv. Szarvasi-1, Festuca arundinacea, Phleum pratense). The plant cultures were established on 16 April 2019 at the experimental field of the Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice. The organic carbon content was evaluated depending on the sampling depth and the fertilization variant. Soil sampling was taken at regular intervals with a pedological sounding rod from three depths (0-10 cm, 10-20 cm and 20-30 cm). The samples were adjusted for subsequent analysis on a Primacs SLC Analyzer in the Department of Agroecosystems laboratory. The highest content of total organic carbon was observed at 0-10 cm depth and the lowest at depth of 20-30 cm. Furthermore, a positive effect of fertilization on the total organic carbon content was found.
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Kvalita a množství organické hmoty v půdách energetických rostlin
KOBLIC, Štěpán
The quality and quantity of organic matter are among the essential factors that significantly affect important soil properties. This work is focused on the research of the quality and quantity of organic matter in soils under energy crops - Szarvasi-I. and Dactylis glomerata. The literature search describes concepts that are important for understanding the topic. The practical part deals with regular soil sampling on experimental plots of JČU, and their subsequent treatment. The samples taken were analyzed in the laboratory according to the methodology of Kopecký et al. (2006), which is focused on determining the rate constant of oxidation of primary soil organic matter. Furthermore, the results were statistically evaluated in terms of the month of sampling, fertilization management and grass species. The results show that in terms of the month of soil sampling was the best primary soil organic matter in October 2019. According to the fertilizer management was the best in the variant "Control" and in terms of monitoring the grass species under Szarvasi-I. In terms of interaction of all monitored factors that the best influence on the quality of primary soil organic matter has unfertilized stands of Szarvasi-I
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Organic carbon content in soils of energy crops
BLÁHA, David
The aim of the study was to determine the total content of organic carbon in the soils planted by selected energy plants (Dactylis glomerata, Elymus elongatus subsp. Ponticus, Festuca arundinacea, Phleum pratense). The plant cultures were established on 16. 4. 2019 at the experimental field of the Faculty of agriculture, University of South Bohemia, České Budějovice. The organic carbon content was monitored regularly depending on the depth of sampling and fertilization of variants. The first sampling was carried out before the plant culture establishment. Further samples were taken every 2 months. All of the samples were taken from the depth of 0-10 cm and 10-20 cm by pedological sounding rod. Subsequently, the samples were adjusted for the analysis and examined with the SKALÁR instrument in the laboratory at the Department of Agroecosystems. During the measurement, a sharp increase of organic carbon in the sample taken in the spring of 2020 was observed. A higher content of organic carbon was observed in the samples taken from the depth of 0-10 cm, though, statistically not significant. It was further found, that fertilization influences the total content of organic carbon. The fertilized variants showed higher values than unfertilized. The highest amount of total organic carbon was recorded at the Phleum pretense variant. However, the differences between the plants were not statistically significant.
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Study of physiological changes in plants under stress by zinc ions
Adam, Rostislav ; Soudek, Petr (advisor) ; Petrová, Šárka (referee)
Heavy metals are part of us life for many centuries. Some of them are for living organism neccessary, but in large amount they have toxic effects. So we should decrease amount of heavy metals in the Environment. We have many way to do it. A relatively new way are the phytoremediation. If we would use the phytoremediation, we should know, what they do in plants. We must use specific plants, which are tolerant to certain heavy metal. If we would select a suitable plant, we have to try, how heavy metals in soil solution are toxic to plants. Zinc is no expection, although it is important part of many proteins. In plants it make rusty leaves and reduct aboveground and root biomass production. In hydroponic experiment I investigated that mallow Malva verticillata was very sensitive to low additon of Zn(NO3)2. The toxic efect appeared in 2 weeks. In sorghum Sorghum bicolor zinc show expressive toxic effect at concentration 1 mmol/l. I studied six cultivars of Sorghum bicolor, DSM 14-535, Expres, Honey Graze BMR, Nutri Honey, Sucrosorgho 506 and Sweet Virginia. According EC50 I as- sessed that the most sensitive was Sucrosorgho 506 and very tolerant were Nutri Honey and Sweet Virginia. Cultivar Nutri Honey was characteristic. It had the highest ratio concentration in shoot to concentration in root. I studied...
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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Samples were colected from 4 sites in Czech republic, in three samplings during the vegetation season. Endophytes were isolated from root, stem and leaf. Fungi asociated with spots were isolated from stem and leaf. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. The most common endophytes were Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata a Acremonium strictum...
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Kvalifikace primární půdní organické hmoty podle rychlostní konstanty oxidace v půdách porostů energetických rostlin
SKOBA, David
This Bachelor thesis is focused on monitoring the yield parameters of selected energy plants (Elymus elonganus, Phalaris arundinacea L., Miscanthus × giganteus). First part of the work was performed at the experimental site of the Faculty of Agriculture of the University of South Bohemia in České Budějovice, where long-term experiments were carried out to look at the yields of these plants. I was observing the yield of crops grown for the purpose of incineration, which are harvested once a year before the beginning of the growing season. Research has shown that on a 4-year average the most profitable species is Miscanthus × giganteus. The second practical part of my work was the sampling and analysis of soil samples from these stands and, for comparison, the sampling of soil under permanent grassland. In the laboratory at the Department of Agroecosystems the samples were modified and examined in terms of the quantity and quality of soil organic matter. The analysis shows that the content of humus in soils with energy plants is lower in comparison to the content of these substances in soil under permanent grassland. There were no significant differences in the observed values of the velocity constants of the oxidation of the primary soil organic matter.
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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Samples were colected from 4 sites in Czech republic, in three samplings during the vegetation season. Endophytes were isolated from root, stem and leaf. Fungi asociated with spots were isolated from stem and leaf. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. The most common endophytes were Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata a Acremonium strictum...
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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Nováková, Alena (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. In total 24 morphotypes of endophytic fungi were cultivated from healthy plants. The most common species were: Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata and Acremonium strictum. From the spots were isolated in total 29 morphotypes, the dominant species were: A. strictum, Aureobasidium pullulans, C....
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Study of accumulation of cadmium ion by energy crops
Berkyová, Petra ; Soudek, Petr (advisor) ; Petrová, Šárka (referee)
Cadmium is heavy metal toxic for plants and animals and environmental contaminant which must be removed from natural environment. In recent years a new method phytoremediation is getting more attention. This method uses plants called hyperaccumulators for extraction of heavy metals from soils. Hyperaccumulators have, however, after accumulation of heavy metals no other use. Therefore new possibilities are discussed in last few years. Energy plants, in this thesis sorghum and malva, could be used for accumulation of heavy metals from soils and after that these plants could be used as energy source. This thesis wants to find out if sorgum and malva are able to grow in cadmium contaminated environment and if these plants will accumulate cadmium. It also compares different cultivars of sorghum in toxicity tests and compares ability of these cultivars to grow in cadmium contaminated environment and to accumulate this heavy metal. Further it focuses on affection of uptake of kadmium ions by sorhum in presence of glutathione or ethylenediaminetetraacetic acid.
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Study of physiological changes in plants under stress by zinc ions
Adam, Rostislav ; Soudek, Petr (advisor) ; Petrová, Šárka (referee)
Heavy metals are part of us life for many centuries. Some of them are for living organism neccessary, but in large amount they have toxic effects. So we should decrease amount of heavy metals in the Environment. We have many way to do it. A relatively new way are the phytoremediation. If we would use the phytoremediation, we should know, what they do in plants. We must use specific plants, which are tolerant to certain heavy metal. If we would select a suitable plant, we have to try, how heavy metals in soil solution are toxic to plants. Zinc is no expection, although it is important part of many proteins. In plants it make rusty leaves and reduct aboveground and root biomass production. In hydroponic experiment I investigated that mallow Malva verticillata was very sensitive to low additon of Zn(NO3)2. The toxic efect appeared in 2 weeks. In sorghum Sorghum bicolor zinc show expressive toxic effect at concentration 1 mmol/l. I studied six cultivars of Sorghum bicolor, DSM 14-535, Expres, Honey Graze BMR, Nutri Honey, Sucrosorgho 506 and Sweet Virginia. According EC50 I as- sessed that the most sensitive was Sucrosorgho 506 and very tolerant were Nutri Honey and Sweet Virginia. Cultivar Nutri Honey was characteristic. It had the highest ratio concentration in shoot to concentration in root. I studied...
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